The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor ce
The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.
대표청구항▼
1. A method of detecting a nucleic acid sequence in a sample comprising the steps of: a) combining the sample with at least one antigen-conjugated oligonucleotide under conditions suitable for hybridization of the antigen-conjugated oligonucleotide to the nucleic acid sequence;b) adding an emittor c
1. A method of detecting a nucleic acid sequence in a sample comprising the steps of: a) combining the sample with at least one antigen-conjugated oligonucleotide under conditions suitable for hybridization of the antigen-conjugated oligonucleotide to the nucleic acid sequence;b) adding an emittor cell comprising one or more receptors and an emittor molecule, wherein the one or more receptors bind to the antigen of the antigen-conjugated oligonucleotides and results in an increase in intracellular calcium, and said emittor molecule emits a photon in response to the increase in intracellular calcium; andc) measuring photon emission from the emittor cell, thereby detecting a nucleic acid sequence in a sample. 2. The method of claim 1, wherein the emittor molecule is a calcium-sensitive luminescent molecule or a calcium-sensitive fluorescent molecule. 3. The method of claim 2, wherein the emittor molecule is selected from the group consisting of aequorin, obelin, thalassicolin, mitrocomin, clytin, mnemiopsin, berovin, Indo-1, Fura-2, Quin-2, Fluo-3, Rhod-2, calcium green, BAPTA, a cameleon and combinations thereof. 4. The method of claim 1, wherein the emittor cell is a cell selected from the group consisting of a prokaryotic cell, a eukaryotic cell and a non-living cell. 5. The method of claim 4, wherein the emittor cell is selected from the group consisting of a B-cell, a T-cell, a macrophage cell, a mast cell and a fibroblast. 6. The method of claim 1, wherein the sample is combined with a plurality of antigen-conjugated oligonucleotides. 7. A method of detecting a nucleic acid sequence in a sample comprising the steps of: a) combining: i) the sample;ii) at least one antigen-conjugated oligonucleotide that is complementary to the nucleic acid sequence; andiii) a solid substrate comprising at least one oligonucleotide complementary to the nucleic acid sequence bound to the solid substrate;under conditions suitable for hybridization of the antigen-conjugated oligonucleotide and the oligonucleotide bound to the solid substrate for hybridizing to the nucleic acid sequence, thereby producing a solid substrate comprising the nucleic acid sequence having at least one hybridized antigen-conjugated oligonucleotide;b) adding to the solid substrate comprising the nucleic acid sequence having at least one hybridized antigen-conjugated oligonucleotide an emittor cell comprising one or more receptors and an emitter molecule, wherein the one or more receptors bind to the antigen of the antigen-conjugated oligonucleotides and results in an increase in intracellular calcium, and said emittor molecule emits a photon in response to the increase in intracellular calcium; andc) measuring photon emission from the emittor cell, thereby detecting a nucleic acid sequence in a sample. 8. The method of claim 7, wherein the emittor molecule is a calcium-sensitive luminescent molecule or a calcium-sensitive fluorescent molecule. 9. The method of claim 8, wherein the emittor molecule is selected from the group consisting of aequorin, obelin, thalassicolin, mitrocomin, clytin, mnemiopsin, berovin, Indo-1, Fura-2, Quin-2, Fluo-3, Rhod-2, calcium green, BAPTA, a cameleon and combinations thereof. 10. The method of claim 8, wherein the emittor cell is a cell selected from the group consisting of a prokaryotic cell, a eukaryotic cell and a non-living cell. 11. The method of claim 10, wherein the emittor cell is selected from the group consisting of a B-cell, a T-cell, a macrophage cell, a mast cell and a fibroblast. 12. A method of detecting a nucleic acid sequence in a sample comprising the steps of: a) combining the sample with at least one antigen-conjugated oligonucleotide under conditions suitable for hybridization of the antigen-conjugated oligonucleotide to the nucleic acid sequence thereby producing an antigen-conjugated hybridization complex;b) adding one or more antibodies specific for the antigen of the antigen-conjugated hybridization complex;c) adding an emittor cell comprising an Fc receptor and an emittor molecule, wherein binding of the Fc receptor to the one or more antibodies results in an increase in intracellular calcium, and wherein said emittor molecule emits a photon in response to the increase in intracellular calcium; andd) measuring photon emission from the emittor cell, thereby detecting a target particle in a sample. 13. The method of claim 12, wherein the emittor molecule is a calcium-sensitive luminescent molecule or a calcium-sensitive fluorescent molecule. 14. The method of claim 13, wherein the emittor molecule is selected from the group consisting of aequorin, obelin, thalassicolin, mitrocomin, clytin, mnemiopsin, berovin, Indo-1, Fura-2, Quin-2, Fluo-3, Rhod-2, calcium green, BAPTA, a cameleon and combinations thereof. 15. The method of claim 12, wherein the emittor cell is a cell selected from the group consisting of a prokaryotic cell, a eukaryotic cell and a non-living cell. 16. The method of claim 15, wherein the emittor cell is selected from the group consisting of a B-cell, a T-cell, a macrophage cell, a mast cell and a fibroblast.
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