최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0024261 (2013-09-11) |
등록번호 | US-8883455 (2014-11-11) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 1 인용 특허 : 342 |
An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette o
An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.
1. A method for detecting the presence of a nucleic acid in a sample, the method comprising performing within a housing of a self-contained, stand-alone analyzer the automated steps of: a) contacting the sample with a solid support such that a complex comprising the nucleic acid and the solid suppor
1. A method for detecting the presence of a nucleic acid in a sample, the method comprising performing within a housing of a self-contained, stand-alone analyzer the automated steps of: a) contacting the sample with a solid support such that a complex comprising the nucleic acid and the solid support is formed, wherein the solid support comprises a magnetically-responsive particle, and wherein the complex is suspended in a fluid component of the sample;b) after step a), subjecting the sample containing the complex to a magnetic field;c) while the sample containing the complex is subjected to the magnetic field, aspirating at least a portion of the fluid component of the sample from the complex, thereby purifying the nucleic acid;d) forming a reaction mixture with a pipette of the analyzer, wherein the reaction mixture comprises the nucleic acid purified in step c) and all reagents required to perform a nucleic acid amplification;e) synthesizing amplification products in the reaction mixture, each of the amplification products comprising a nucleotide sequence contained in the nucleic acid or its complement;f) exposing an amplification product that is one of the amplification products synthesized in step e) to a probe, such that a hybrid comprising the probe and the amplification product is formed in a mixture containing the probe and the amplification products; andg) detecting the formation of the hybrid, wherein the formation of the hybrid in the mixture is an indication of the presence of the nucleic acid in the sample. 2. The method of claim 1, wherein the probe comprises a detectable label, and wherein step g) comprises detecting the label in the mixture after formation of the hybrid. 3. The method of claim 2, further comprising, prior to step a), a manual step of providing the sample and the reagents to the analyzer, wherein the sample and the reagents are separately provided to the analyzer. 4. The method of claim 2, wherein the complex formed in step a) further comprises a capture probe hybridized to the nucleic acid. 5. The method of claim 2, further comprising performing within the housing an automated step of washing the solid support one or more times after step c) and prior to step d). 6. The method of claim 2, wherein the fluid component is aspirated through a tiplet in frictional engagement with an aspirator tube of a fluid aspirator of the analyzer. 7. The method of claim 2, further comprising, prior to step c), an automated step of sensing the level of the fluid component. 8. The method of claim 2, wherein the portion of the fluid component aspirated in step c) is drawn through an aspirator tube to a fluid waste container. 9. The method of claim 8, wherein the fluid waste container is situated in a lower chassis of the analyzer beneath a processing deck contained within the housing. 10. The method of claim 2, wherein the steps of the method are performed in a single receptacle. 11. The method of claim 10, wherein the receptacle is a cylindrical tube. 12. The method of claim 10, wherein the receptacle is one of a plurality of integrally formed receptacles. 13. The method of claim 2, wherein the amplification products formed in step e) comprise amplification products having the nucleotide sequence contained in the nucleic acid and amplification products having the nucleotide sequence contained in the complement of the nucleic acid. 14. The method of claim 2, wherein step e) is performed in a chamber defined by an enclosure comprising a receptacle access opening, and wherein the receptacle access opening is closed by a door of the enclosure during step e). 15. The method of claim 14, wherein the chamber is maintained at a substantially uniform temperature during step e). 16. The method of claim 2, wherein the probe hybridizes with specificity to the amplification product in step f), and wherein the nucleic acid is from a disease-associated pathogen. 17. The method of claim 2, wherein the hybrid is formed in solution in the mixture of step f). 18. The method of claim 2, wherein the label is a fluorescent or chemiluminescent label. 19. The method of claim 2, wherein steps b) and c) are performed at a first station of the analyzer and step e) is performed at a second station of the analyzer. 20. The method of claim 19, wherein step e) is performed in a chamber defined by an enclosure comprising a receptacle access opening, and wherein the receptacle access opening is closed by a door of the enclosure during step e). 21. The method of claim 19, wherein the first and second stations are situated on a processing deck contained within the housing. 22. The method of claim 2, wherein steps e) and f) are performed at separate stations situated within the housing. 23. The method of claim 22, wherein step e) is performed in a first incubator and step f) is performed in a second incubator, and wherein the first and second incubators are in a laterally spaced-apart relationship. 24. The method of claim 22, wherein the stations are situated on a processing deck contained within the housing. 25. The method of claim 2, wherein the analyzer is controlled by a computer controller that is integrally housed within the analyzer. 26. The method of claim 2, wherein the steps of the method are performed at multiple stations situated on a processing deck contained within the housing. 27. The method of claim 2, wherein the housing remains closed during the method.
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