Compositions for enhancing the antibacterial activity of myeloperoxidase and methods of use thereof
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-038/44
A61K-045/06
A61L-002/00
출원번호
US-0118586
(2008-05-09)
등록번호
US-8945540
(2015-02-03)
발명자
/ 주소
Becquerelle, Sophie
Haag, William
Abril-Horpel, Obsidiana
Valvani, Shri
출원인 / 주소
Exoxemis, Inc.
대리인 / 주소
Betts, Patterson & Mines, P.S.
인용정보
피인용 횟수 :
2인용 특허 :
18
초록
Methods and compositions are provided for inhibiting the growth of susceptible microorganisms by contacting the microorganisms, in the presence of a peroxide and chloride or bromide, with myeloperoxidase and at least two activity enhancing agent amino acids.
대표청구항▼
1. A composition for inhibiting the growth of susceptible microorganisms comprising myeloperoxidase, glucose oxidase, sodium chloride, polysorbate 80, phosphate buffer, and two amino acids, wherein one of the two amino acids is glycine, and the other of the two amino acids is selected from the group
1. A composition for inhibiting the growth of susceptible microorganisms comprising myeloperoxidase, glucose oxidase, sodium chloride, polysorbate 80, phosphate buffer, and two amino acids, wherein one of the two amino acids is glycine, and the other of the two amino acids is selected from the group consisting of D-isoleucine; L-isoleucine; L-tyrosine; and L-valine; and wherein the two amino acids work in combination to enhance the microbicidal activity of the myeloperoxidase. 2. The composition of claim 1, wherein glucose oxidase is present in an amount effective to generate from 100 pmol to 50 μmol peroxide per ml per minute when in the presence of a substrate for the oxidase. 3. The composition of claim 1 which comprises from 1 to 50,000 μg/ml of myeloperoxidase. 4. The composition of claim 1 which comprises from 0.1 to about 500 mM of each of the two amino acids. 5. A composition for killing or inhibiting the growth of susceptible microorganisms, wherein the composition consists of myeloperoxidase, glycine, L-alanine, L-proline, glucose oxidase, sodium chloride, polysorbate-80, sodium phosphate buffer, and water, wherein the glycine, L-alanine, and L-proline in the composition work in combination to enhance the microbicidal activity of the myeloperoxidase. 6. A method of treating a microbial infection in a human or animal subject, comprising administering to a site of infection in the subject a composition of claim 1. 7. The method of claim 6 wherein the composition comprises glucose oxidase in an amount effective to generate from 100 pmol to 50 μmol peroxide per ml per minute when in the presence of a substrate for the oxidase. 8. The method of claim 6 wherein the composition comprises from 1 to 50,000 μg/ml of myeloperoxidase. 9. The method of claim 6 wherein the composition comprises from 0.1 to about 500 mM of each of the two amino acids. 10. The method of claim 6 wherein the human or animal subject is suffering from a microbial infection of the gums, eyes, ears, skin, soft tissue, wounds, vaginal areas, groin areas, bed sores or burn areas. 11. The method of claim 10 wherein the infection is a polymicrobial infection. 12. A method for killing or inhibiting the growth of susceptible microorganisms comprising contacting the microorganisms with the composition of claim 1. 13. A binary combination, comprising: (a) a first composition comprising myeloperoxidase, glucose oxidase, sodium chloride, polysorbate-80, phosphate buffer, water and two amino acids, wherein one of the two amino acids is glycine, and the other of the two amino acids is selected from the group consisting of D-isoleucine; L-isoleucine; L-tyrosine; and L-valine; and wherein the two amino acids work in combination to enhance the microbicidal activity of the myeloperoxidase; and(b) a second composition comprising glucose and water. 14. A binary combination for killing or inhibiting the growth of susceptible microorganisms, consisting of: (a) a first composition consisting of myeloperoxidase, glycine, L-alanine, L-proline, glucose oxidase, sodium chloride, polysorbate-80, sodium phosphate buffer, and water, wherein the glycine, L-alanine, and L-proline in the composition work in combination to enhance the microbicidal activity of the myeloperoxidase; and(b) a second composition comprising glucose and water. 15. A method of treating a microbial infection in a human or animal subject, comprising administering to a site of infection in the subject a composition of claim 5. 16. A method for killing or inhibiting the growth of susceptible microorganisms comprising contacting the microorganisms with the composition of claim 5. 17. The method of claim 15 wherein the human or animal subject is suffering from a microbial infection of the gums, eyes, ears, skin, soft tissue, wounds, vaginal areas, groin areas, bed sores or burn areas. 18. The method of claim 17 wherein the infection is a polymicrobial infection. 19. The composition of claim 5, wherein the myeloperoxidase is present in a concentration front bout 10 μg/ml to about 5,000 μg/ml. 20. The composition of claim 5, wherein each of the glycine, L-alanine, and L-proline is present in a concentration from about 0.3 mM to about 50 mM. 21. The composition of claim 5, wherein the glucose oxidase is present in a concentration from about 1 U/ml to about 500 U/ml.
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