A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syri
A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.
대표청구항▼
1. A method for isolating nucleic acid, comprising: (a) loading a multicapillary element retained in or attached to a pipette, micropipette, pipette tip, or syringe device, with a loading solution comprising nucleic acid under conditions that permit adsorption of the nucleic acid to the inner wall o
1. A method for isolating nucleic acid, comprising: (a) loading a multicapillary element retained in or attached to a pipette, micropipette, pipette tip, or syringe device, with a loading solution comprising nucleic acid under conditions that permit adsorption of the nucleic acid to the inner wall of the capillaries, which multicapillary element comprises a plurality of parallel capillaries comprising a substantially imperforate inner wall;(b) discarding the loading solution from the device after (a); and(c) eluting the nucleic acid from the multicapillary element after (b), whereby the nucleic acid is isolated from the loading solution. 2. The method of claim 1, wherein the device comprises a plastic, glass, fused silica, ceramic, or stainless steel. 3. The method of claim 2, wherein the plastic is formed from polyetheretherketone, polystyrene, polypropylene or polyethylene. 4. The method of claim 3, wherein the device is formed from polypropylene. 5. The method of claim 1, wherein the device is a pipette tip. 6. The method of claim 5, wherein the pipette tip is formed from polystyrene, polypropylene or polyethylene. 7. The method of claim 6, wherein the pipette tip is formed from polypropylene. 8. The method of claim 1, wherein the capillaries are substantially cylindrical. 9. The method of claim 1, wherein the cross section of the capillaries is substantially polygonal. 10. The method of claim 1, wherein the cross section of the capillaries is substantially hexagonal. 11. The method of claim 1, wherein the multicapillary element comprises fused silica, glass, ceramic, stainless steel, polystyrene, polypropylene, polyethylene, or polyetheretherketone. 12. The method of claim 11, wherein the multicapillary element consists of fused silica. 13. The method of claim 11, wherein the multicapillary element consists of glass. 14. The method of claim 1, wherein an inner diameter of each capillary is about 0.1 micrometers to about 200 micrometers. 15. The method of claim 1, wherein an outer diameter of the multicapillary element is about 1 mm to about 20 mm. 16. The method of claim 1, wherein the length of the multicapillary element is about 1 mm to about 250 mm. 17. The method of claim 1, wherein the volume of the device is about 1 microliter to about 100 microliters. 18. The method of claim 1, wherein the inner wall of the capillaries includes particles of inert material. 19. The method of claim 1, wherein the inner wall of the capillaries includes a nodular surface. 20. The method of claim 1, wherein the inner wall of the capillaries is etched. 21. The method of claim 1, wherein the nucleic acid comprises deoxyribonucleic acid (DNA). 22. The method of claim 1, wherein the nucleic acid comprises ribonucleic acid (RNA). 23. The method of claim 1, wherein the nucleic acid isolated is sheared less than nucleic acid isolated from a device that includes porous silica. 24. The method of claim 1, further comprising washing the multicapillary element after the loading solution is discarded and before the nucleic acid is eluted from the multicapillary element. 25. The method of claim 1, wherein the inner surface of each capillary comprises an insoluble stationary phase coated directly on the inner wall of the capillaries. 26. The method of claim 25, wherein the stationary phase comprises C18.
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Woodard Daniel L. (Raleigh NC) Howard Adriann J. (Raleigh NC) Down James A. (Cary NC), DNA purification by solid phase extraction using a PCl3modified glass fiber membrane.
Woodard Daniel L. (Raleigh NC) Howard Adriann J. (Raleigh NC) Down James A. (Cary NC), DNA purification by solid phase extraction using a hydroxide-washed glass fiber membrane.
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Goffe Randal A. (Medway MA) Zale Stephen E. (Marlborough MA) O\Connor James L. (Chelmsford MA) Kessler Stephen B. (Princeton MA), Membrane affinity apparatus and purification methods related thereto.
Schleifer Arthur ; Caren Michael P. ; Leonard Leslie A. ; Hotz Charles Z. ; Perbost Michel G. M., Method and apparatus for fabricating replicate arrays of nucleic acid molecules.
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Ohnaka Tadao (Shinnanyo) Komiya Katsuo (Shinnanyo) Moriyama Hiroyuki (Shinnanyo JPX), Packing material for reversed phase chromatography and process for its preparation.
Puchinger Herwig (Hamburg DEX) Gerken Hero (Hamburg DEX) Khn Gnther (Hamburg DEX), Pipetting device comprising a retaining cone for holding a slip-on pipette tip and pipette tip for such pipetting device.
Cussler Edward L. (Edina MN) Gillberg-LaForce Gunilla E. (Summit NJ) Sansone Michael J. (Berkeley Heights NJ) Schisla David K. (St. Louis MO), Process for making microporous membranes having gel-filled pores, and separations methods using such membranes.
Ward Robert R. (Cary NC) Chang Richard C. (Raleigh NC) Danos James C. (Chapel Hill NC) Carden ; Jr. Joseph A. (Durham NC), Processes for coating bundles of hollow fiber membranes.
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