A chamber in which an agent, like genomic DNA, may be harvested and optionally manipulated rapidly (e.g., on the order of a few hours), without shearing or fragmentation.
대표청구항▼
1. A system for separating agents from non-agents in a chamber, the system comprising: a chamber comprising: a fluid introduction port;a porous substrate positioned substantially opposite from the fluid introduction port in the chamber, the porous substrate configured to prevent the passage of agent
1. A system for separating agents from non-agents in a chamber, the system comprising: a chamber comprising: a fluid introduction port;a porous substrate positioned substantially opposite from the fluid introduction port in the chamber, the porous substrate configured to prevent the passage of agents that are larger than a threshold size of the porous substrate, the porous substrate having a first side and a second side, wherein the fluid introduction port is positioned on the first side of the porous substrate;a plurality of ports coupled to the chamber and configured to permit fluid to exit the chamber, wherein the plurality of ports includes at least a first port, a second port, and a third port, wherein the first port and the second port are positioned on the first side of the porous substrate and the third port is positioned on the second side of the porous substrate, and wherein the first port is substantially parallel to a surface of the porous substrate;a controller configured to control: a first flow of fluid into the chamber from the fluid introduction port and through the porous substrate to hold agents and other constituents at the porous substrate; anda second flow of fluid into the chamber from the fluid introduction port and through at least one of the first and second ports to allow other constituents to move away from the agents on the porous substrate. 2. The system of claim 1, wherein the controller is configured to reduce the second flow relative to the first flow to direct agents towards the porous substrate when agents are introduced to the chamber through the fluid introduction port. 3. The system of claim 1, wherein the controller is configured to increase the second flow relative to the first flow to move other constituents away from the agents on the porous substrate. 4. The system of claim 1, wherein the controller is configured to substantially stop the first and second flows through the chamber to allow reactions to occur between the agents and other constituents. 5. The system of claim 1, wherein the controller is configured to reverse the first flow to move the agents away from the porous substrate and towards the fluid introduction port. 6. The system of claim 1, wherein the agents comprise nucleic acids that have been separated from one or more cells from which the nucleic acids were originally presented. 7. The system of claim 1, wherein the other constituents are selected from the group comprising: sequence specific probes, intercalators, cellular debris, lytic enzymes, endonucleases, and exonucleases. 8. The system of claim 1, wherein the agents comprise proteins. 9. The system of claim 1, wherein the controller is configured to automatically control the first flow and the second flow according to an operating protocol. 10. The system of claim 1, wherein the chamber has a substantially truncated cone shape. 11. A system for separating agents from non-agents in a chamber, the system comprising: a chamber comprising: a fluid introduction port;a porous substrate positioned substantially opposite from the fluid introduction port in the chamber, the porous substrate configured to prevent the passage of agents that are larger than a threshold size of the porous substrate, the porous substrate having a first side and a second side, wherein the fluid introduction port is positioned on the first side of the porous substrate;a plurality of ports coupled to the chamber and configured to permit fluid to exit the chamber, wherein the plurality of ports includes at least a first port, a second port, a third port and a fourth port, wherein the first port and the second port are positioned on the first side of the porous substrate and the third port and the fourth port are positioned on the second side of the porous substrate;a controller configured to control: a first flow of fluid into the chamber from the fluid introduction port and through the porous substrate to hold agents and other constituents at the porous substrate; anda second flow of fluid into the chamber from the fluid introduction port and through at least one of the first and second ports to allow other constituents to move away from the agents on the porous substrate. 12. The system of claim 11, wherein the chamber has a substantially truncated cone shape. 13. A system for separating agents from non-agents in a chamber, the system comprising: a chamber with a substantially truncated cone shape, the chamber comprising: a fluid introduction port;a porous substrate positioned substantially opposite from the fluid introduction port in the chamber, the porous substrate configured to prevent the passage of agents that are larger than a threshold size of the porous substrate, the porous substrate having a first side and a second side, wherein the fluid introduction port is positioned on the first side of the porous substrate;a plurality of ports coupled to the chamber and configured to permit fluid to exit the chamber, wherein the plurality of ports includes at least a first port, a second port and a third port, wherein the first port and the second port are positioned on the first side of the porous substrate and the third port is positioned on the second side of the porous substrate;a controller configured to control:a first flow of fluid into the chamber from the fluid introduction port and through the porous substrate to hold agents and other constituents at the porous substrate; anda second flow of fluid into the chamber from the fluid introduction port and through at least one of the first and second ports to allow other constituents to move away from the agents on the porous substrate,wherein the controller is configured to reduce the second flow relative to the first flow to direct agents towards the porous substrate when agents are introduced to the chamber through the fluid introduction port. 14. A system for separating agents from non-agents in a chamber, the system comprising: a chamber with a substantially truncated cone shape, the chamber comprising: a fluid introduction port;a porous substrate positioned substantially opposite from the fluid introduction port in the chamber, the porous substrate configured to prevent the passage of agents that are larger than a threshold size of the porous substrate, the porous substrate having a first side and a second side, wherein the fluid introduction port is positioned on the first side of the porous substrate;a plurality of ports coupled to the chamber and configured to permit fluid to exit the chamber, wherein the plurality of ports includes at least a first port, a second port and a third port, wherein the first port and the second port are positioned on the first side of the porous substrate and the third port is positioned on the second side of the porous substrate;a controller configured to control:a first flow of fluid into the chamber from the fluid introduction port and through the porous substrate to hold agents and other constituents at the porous substrate; anda second flow of fluid into the chamber from the fluid introduction port and through at least one of the first and second ports to allow other constituents to move away from the agents on the porous substrate,wherein the controller is configured to increase the second flow relative to the first flow to move other constituents away from the agents on the porous substrate.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (113)
Yager Paul ; Brody James P., Absorption-enhanced differential extraction device.
Cheng Jing ; Sheldon ; III Edward L. ; Wu Lei ; O'Connell James P., Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis.
Liao Jia-li (Uppsala SEX) Hjerten Stellan (Uppsala CA SEX) Siebert Christopher (Berkeley CA), Concentration of biological samples on a microliter scale and analysis by capillary electrophoresis.
Liao Jia-li,SEX ; Hjerten Stellan,SEX ; Siebert Christopher, Concentration of biological samples on a microliter scale and analysis by capillary electrophoresis.
Cathey Cheryl A. ; Saul Tom ; Bloom Nicole D. ; Ribi Hans O. ; Schwartz Henry L. ; Langford Jeffrey B. ; Paul David J., Device for use in analyte detection assays.
Mian Alec ; Kieffer-Higgins Stephen G. ; Corey George D., Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system.
Mathies Richard A. ; Glazer Alexander N. ; Lao Kaiqin ; Woolley Adam T., Electrochemical detector integrated on microfabricated capillary electrophoresis chips.
Maley Thomas C. (Medway MA) D\Orazio Paul A. (Mendon MA) Dalzell Bonnie C. (Sherborn MA) Edelman Peter G. (Franklin MA) Flaherty James E. (Attleboro MA) Mason Richard W. (Millis MA) McCaffrey Robert , Electrochemical sensors.
Maley Thomas C. ; D'Orazio Paul A. ; Dalzell Bonnie C. ; Edelman Peter G. ; Flaherty James E. ; Mason Richard W. ; McCaffrey Robert R., Electrochemical sensors membrane.
Paul Phillip H. ; Rakestraw David J. ; Arnold Don W. ; Hencken Kenneth R. ; Schoeniger Joseph S. ; Neyer David W., Electrokinetic high pressure hydraulic system.
Paul, Phillip H.; Rakestraw, David J.; Arnold, Don W.; Hencken, Kenneth R.; Schoeniger, Joseph S.; Neyer, David W., Electrokinetic high pressure hydraulic system.
Doering Don S. (Somerville MA) Matsudaira Paul T. (Boston MA), Electrophoresis apparatus and method for electroeluting desired molecules for further processing.
Etani Kenji (W. Meadow Road West Townsend MA 01474) Etani Nancy A. (W. Meadow Road West Townsend MA 01474), Filtration system having prefilter and main filter.
Wada, H. Garrett; Kopf-Sill, Anne R.; Alajoki, Marja Liisa; Parce, J. Wallace; Wang, Benjamin N.; Chow, Andrea W.; Dubrow, Robert S., Focusing of microparticles in microfluidic systems.
Gilbert,John R.; Deshpande,Manish; Trikha,Jaishree, Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system.
Becker, Frederick F.; Gascoyne, Peter R. C.; Huang, Ying; Wang, Xiao-Bo; Yang, Jun, Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation.
Becker Frederick F. ; Gascoyne Peter R. C. ; Huang Ying ; Wang Xiao-Bo, Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation.
Soane David S. (Piedmont CA) Soane Zoya M. (Piedmont CA), Method and device for moving molecules by the application of a plurality of electrical fields.
Ramsey, J. Michael; Foote, Robert S., Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid probes.
Zhao,Xiaojian (David); Randall,Jeffrey D.; Kundu,Bijit; Kesty,Jessica; Gullans,Steven R.; Chan,Eugene Y.; Fuchs,Martin; Rooke,Jenny E., Methods and apparati using single polymer analysis.
Jacobson Stephen C. ; Ramsey J. Michael, Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same.
Austin Robert H. (Princeton NJ) Volkmuth Wayne D. (Menlo Park MN) Rathburn Lynn C. (Ithaca NY), Microlithographic array for macromolecule and cell fractionation.
Fishman Harvey A. (Stanford CA) Shear Jason B. (Menlo Park CA) Colon Luis A. (Menlo Park CA) Zare Richard N. (Stanford CA) Sweedler Jonathan V. (Champaign IL), On-column derivatization in capillary electrophoresis.
Giddings John C. (Salt Lake City UT), Pinched channel inlet system for reduced relaxation effects and stopless flow injection in field-flow fractionation.
Tomblin Graham J. (Tappan NY) Wexler Karen B. (Harrison NY) Ford John P. (Tappan NY) Fischer Stuart G. (New York NY), Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA.
Chow Calvin Y. H. ; Parce J. Wallace, Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forc.
Chow Calvin Y. H. ; Parce J. Wallace, Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forc.
Calvin Y. H. Chow ; J. Wallace Parce, Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.