System and methods for making and processing emulsions
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12M-001/00
C12M-003/00
C12M-001/34
C12M-001/36
C12M-001/38
B01F-003/08
B01F-005/04
B01F-013/00
B04B-005/04
B04B-007/02
B01L-003/00
출원번호
US-0442547
(2012-04-09)
등록번호
US-9017993
(2015-04-28)
발명자
/ 주소
Schultz, Jonathan
Nobile, John
Reed, Brian
Thwar, Prasanna
Roswech, Todd
출원인 / 주소
Life Technologies Corporation
인용정보
피인용 횟수 :
2인용 특허 :
10
초록▼
An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generatio
An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
대표청구항▼
1. A system for making membrane-based emulsion droplets, comprising: an emulsion-generating device comprising a housing and a top channel gasket, a bottom channel gasket, and an emulsion-generating membrane disposed between top channel gasket and bottom channel gasket disposed within the housing, a
1. A system for making membrane-based emulsion droplets, comprising: an emulsion-generating device comprising a housing and a top channel gasket, a bottom channel gasket, and an emulsion-generating membrane disposed between top channel gasket and bottom channel gasket disposed within the housing, a first chamber defined between top channel gasket and emulsion-generating membrane, and a second chamber defined between emulsion-generating membrane and bottom channel gasket, the housing defining a sample inlet port, a carrier fluid inlet port, an emulsion outlet port, and a displacement fluid inlet port, the sample inlet port disposed on an upper surface of the housing, the displacement fluid inlet port disposed on a lower surface of the housing; a microreactor mixture supply in the form of a sample tube in communication with the top channel gasket through the sample inlet port; a carrier fluid supply in communication with the bottom channel gasket through the carrier fluid inlet port; and an emulsion collection device in communication with the emulsion outlet port and the top or bottom channel gasket for collecting the membrane-based emulsion droplets; wherein the emulsion-generating membrane comprises at least two through holes; and wherein a passageway is defined between the displacement fluid inlet port and the sample tube through the first and second channel gaskets and the membrane. 2. The system of claim 1, wherein each of the membrane-based emulsion droplets comprises a volume of a microreactor mixture at least partially surrounded by the carrier liquid, and the carrier liquid is immiscible with the microreactor mixture. 3. The system of claim 1, wherein the top channel gasket comprises at least one flow path defined therein. 4. The system of claim 3, wherein the at least one flow path is defined in a bottom surface of the top channel gasket. 5. The system of claim 1, wherein the bottom channel gasket comprises at least one flow path defined therein. 6. The system of claim 5, wherein the at least one flow path is defined in a top surface of the top channel gasket. 7. The system of claim 1, wherein at least some of the membrane-based emulsion droplets comprise a microreactor. 8. The system of claim 1, wherein the microreactor supply mixture and the carrier fluid supply are pressurized. 9. The system of claim 1, wherein the second chamber comprises a volume of carrier liquid and at least one emulsion droplet. 10. An emulsion generating system comprising: an emulsion generating device comprising: a housing defining a sample inlet port, a carrier fluid inlet port, an emulsion outlet port, and a displacement fluid inlet port; first and second channel gaskets; and a membrane disposed between the first and second channel gaskets; and a sample tube disposed on the sample inlet port and in fluid communication with the displacement fluid inlet port; wherein the sample inlet port is disposed on an upper surface of the housing; and wherein the displacement fluid inlet port is disposed on a lower surface of the housing, a passageway defined between the displacement fluid inlet port and the sample tube through the first and second channel gaskets and the membrane. 11. The emulsion generating system of claim 10, wherein the carrier fluid inlet port and the emulsion outlet port are disposed on a lower surface of the housing. 12. The emulsion generating system of claim 10, wherein the first and second channel gaskets define a first set and a second set of complementary channels. 13. The emulsion generating system of claim 12, wherein the first and second set of complementary channels are concurrently complementary. 14. The emulsion generating system of claim 12, wherein the first and second set of complementary channels are countercurrently complementary. 15. The emulsion generating system of claim 12, wherein the first and second set of complementary channels have a depth of not greater than 500 micrometers. 16. A method of generating an emulsion, the method comprising: applying an aqueous sample solution to the sample tube of the emulsion generating system of claim 10,applying a displacement fluid through the displacement fluid inlet port;applying a carrier fluid through the carrier fluid inlet port; andcollecting an emulsion through the emulsion outlet port. 17. The method of claim 16, wherein the displacement fluid and the carrier fluid comprise an oil. 18. The method of claim 16, wherein the displacement fluid displaces the aqueous sample fluid from the sample tube.
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