초록 ▼

The conductive polymer films of this disclosure reversibly and selectively mediate ligand-receptor interactions. This electrochemical manipulation of biochemical interactions is accomplished by embedding or adsorbing receptors for ligands of interest in or onto a conductive polymer matrix. The matri

The conductive polymer films of this disclosure reversibly and selectively mediate ligand-receptor interactions. This electrochemical manipulation of biochemical interactions is accomplished by embedding or adsorbing receptors for ligands of interest in or onto a conductive polymer matrix. The matrix can also be doped, for example, with desired ions, polyions, or surfactants. Depending on the receptor properties and dopants utilized, ligand-receptor interactions at the polymer-electrolyte interface are manipulated by controlling the oxidation and reduction of the conductive polymer. The intrinsic charge transfer characteristics of conductive polymers are used to modulate ligand-receptor interactions.

대표청구항 ▼

1. A method for selectively moving a target cell, the method comprising: cutting or piercing tissue using a device configured to cut or pierce tissue,the device comprising: a receptor-doped polymer, the receptor-doped polymer including at least one receptor entrapped in the polymer; anda working ele

1. A method for selectively moving a target cell, the method comprising: cutting or piercing tissue using a device configured to cut or pierce tissue,the device comprising: a receptor-doped polymer, the receptor-doped polymer including at least one receptor entrapped in the polymer; anda working electrode coated with the receptor-doped polymer, wherein the device is a surgical instrument that binds and removes a target cell;contacting a heterogenous population of cells with the device;applying an electrical current to manipulate an oxidation or reduction state of the receptor-doped polymer to modulate binding interactions between the at least one receptor and a ligand associated with the target cell;binding the ligand associated with the target cell to the at least one receptor; anddisplacing the device from a first location to a second location, wherein the target cell is displaced from the heterogenous population. 2. The method of claim 1, wherein the ligand binds to the at least one receptor upon application of a positive charge to the receptor-doped polymer. 3. The method of claim 1, wherein the ligand is released from binding with the at least one receptor upon application of a negative charge to the receptor-doped polymer. 4. The method of claim 1, wherein the at least one receptor is a cell-specific receptor or cell-binding fragment thereof. 5. The method of claim 1, wherein the at least one receptor is an antibody or fragment thereof. 6. The method of claim 1, wherein the at least one receptor binds to a cell surface antigen of the target cell, wherein the target cell is a eukaryotic or prokaryotic cell. 7. The method of claim 1, wherein the at least one receptor binds to a tumor-specific antigen. 8. The method of claim 1, further comprising: releasing the target cell from the receptor-doped polymer after the target cell is displaced. 9. The method of claim 1, wherein applying the electrical current to the receptor-doped polymer enables binding between the at least one receptor and the ligand. 10. The method of claim 1, wherein applying a second electrical current to the receptor-doped polymer enables release of the ligand from binding with the at least one receptor. 11. The method of claim 1, wherein applying the electrical current to the receptor-doped polymer includes applying a first voltage of a first charge to bind the ligand with the at least one receptor and a second voltage of a second opposite charge to release the ligand from binding with the at least one receptor. 12. The method of claim 11, wherein application of one of the first and second voltages manipulates the oxidation or reduction state of the receptor-doped polymer. 13. The method of claim 1, wherein the receptor-doped polymer includes at least one dopant selected from ions, polyions and surfactant molecules embedded in the receptor-doped polymer. 14. The method of claim 13, wherein the at least one dopant is selected from Cl−, NO3−, ClO4−, SO4−, dodecylbenzene sulfonate, Na+, N+, cetyltrimethylammonium chloride, dodecyltrimethylammonium chloride, octyltrimethylammonium chloride, and combinations thereof. 15. The method of claim 1, wherein the at least one receptor is selected from a monoclonal or polyclonal antibody, ssDNA or mRNA sequence, enzyme inhibitor, affinity probe, drug target, protein, biomolecule binding domain, and combinations thereof. 16. The method of claim 15, wherein the at least one receptor is a monoclonal anti-fibronectin antibody. 17. The method of claim 1, wherein the receptor-doped polymer is in the form of a film. 18. The method of claim 1, wherein the receptor-doped polymer has a polyene backbone. 19. The method of claim 18, wherein the polymer is selected from polyacetylene, polyaniline, polypyrrole, polythiopene, and poly(p-phenylene). 20. The method of claim 19, wherein the polymer is polypyrrole. 21. The method of claim 1, wherein the device further comprises a potentiostat electrically coupled with the working electrode. 22. The method of claim 1, wherein the device further comprises a reference electrode and a counter electrode. 23. The method of claim 1, wherein the working electrode is in the form of a scalpel. 24. The method of claim 22, wherein applying the electrical current to the receptor-doped polymer includes applying a voltage to the electrodes to bind the ligand associated with the target cell to the at least one receptor. 25. The method of claim 24, further comprising: changing the voltage applied to the electrodes to release the ligand from the at least one receptor after the target cell is displaced. 26. The method of claim 1, wherein the target cell is inside a human body. 27. The method of claim 1, wherein the target cell is a cancer cell or a bacteria cell. 28. The method of claim 1, wherein the device further comprises a semiconductor chip including: an array of working electrodes each coated with a conductive polymer film including the at least one receptor; andpathways of an electrically conductive material electrically coupled with each working electrode. 29. The method of claim 28, wherein different working electrodes are coated with receptor-doped polymer films including different receptors.