Device for stretching a polymer in a fluid sample
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
B01L-003/00
G01N-001/28
출원번호
US-0862129
(2013-04-12)
등록번호
US-9028776
(2015-05-12)
발명자
/ 주소
Meltzer, Robert H.
Griffis, Joshua W.
출원인 / 주소
Toxic Report LLC
대리인 / 주소
Wolf, Greenfield & Sacks, P.C.
인용정보
피인용 횟수 :
0인용 특허 :
86
초록
The invention provides structures and methods that allow polymers of any length, including nucleic acids, to be stretched into a long, linear conformation for further analysis.
대표청구항▼
1. A device for stretching at least one polymer in a fluid sample, said device comprising: an elongation structure, wherein said elongation structure comprises a tapered channel, said tapered channel decreasing in width from a first end to a second end, said tapered channel comprising: a first zone
1. A device for stretching at least one polymer in a fluid sample, said device comprising: an elongation structure, wherein said elongation structure comprises a tapered channel, said tapered channel decreasing in width from a first end to a second end, said tapered channel comprising: a first zone having a first tapered shape;a second zone having a second tapered shape, wherein the second tapered shape is different than the first tapered shape; andwherein said at least one polymer, when present, moves along said tapered channel from said first end to said second end and is stretched,the first tapered shape includes an increasing strain rate taper, andthe second tapered shape includes a constant strain rate taper. 2. The device of claim 1, wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=w21+xaa=l2w2w3-1wherein l2 is the length of the second zone, w2 is the width of the tapered channel at a transition which separates the first zone from the second zone, and w3 is the width of the tapered channel at the second end of the tapered channel. 3. The device of claim 1, wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=F2xF2=vxwxɛ.wherein x is distance along the tapered channel, F2 is a constant strain rate taper coefficient, vx is fluid velocity at distance x, wx is channel width at distance x, and εx is strain rate at distance x. 4. The device of claim 1, wherein the elongation structure is formed on a chip. 5. The device of claim 1, further comprising a delivery region for delivering said at least one polymer in said fluid sample to said elongation structure. 6. The device of claim 5, wherein said delivery region comprises a sample loading port and a delivery channel, said delivery channel leading into the elongation structure. 7. The device of claim 6, further comprising at least one buffer channel leading into the elongation structure. 8. The device of claim 7, wherein the at least one buffer channel comprises at least two opposing buffer channels leading into the elongation structure. 9. A method of stretching at least one polymer in a fluid sample, the method comprising: delivering a fluid sample into the device recited in claim 1;stretching the at least one polymer in the first zone of the tapered channel; andmaintaining the tension on the at least one polymer in the second zone of the tapered channel. 10. A method comprising: moving a polymer through the device recited in claim 1;detecting an object-dependent impulse that conveys information about structural characteristics of the polymer;obtaining an observed trace based on the detected impulse, wherein the observed trace is an intensity versus time trace;applying an acceleration correction to the observed trace; andobtaining a corrected intensity versus distance trace from the application of the acceleration correction to the observed trace. 11. The method of claim 10, wherein the acceleration correction is defined by: Δxc≅-L22xtagτ(1-τ)wherein:Δxc is a difference in a distance a molecule would travel assuming a constant velocity compared to a distance traveled in an accelerating flow experienced by the polymer;L is a length of the molecule;xtag is a distance of a point of detection from a theoretical asymptotic origin of the constant strain portion of the channel; andτ is a relative time for the molecule to transit a point of detection. 12. A device for stretching at least one polymer in a fluid sample, said device comprising: an elongation structure, wherein said elongation structure comprises a tapered channel, said tapered channel decreasing in width from a first end to a second end, said tapered channel comprising: a first zone having a first tapered shape;a second zone having a second tapered shape, wherein the second tapered shape is different than the first tapered shape; andwherein said at least one polymer, when present, moves along said tapered channel from said first end to said second end and is stretched,wherein the first tapered shape includes an increasing strain rate taper, andwherein a width w(x) of the tapered channel in the first zone is defined by the following equations: w(x)=1(bx+c)2b=1l1(1w2-1w1)c=1w1wherein l1 is the length of the first zone, w1 is the width of the tapered channel at the first end of the tapered channel, and w2 is the width of the tapered channel at a transition which separates the first zone from the second zone. 13. The device of claim 12, wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=w21+xaa=l2w2w3-1wherein l2 is the length of the second zone, w2 is the width of the tapered channel at a transition which separates the first zone from the second zone, and w3 is the width of the tapered channel at the second end of the tapered channel. 14. The device of claim 12, wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=F2xF2=vxwxɛ.wherein x is distance along the tapered channel, F2 is a constant strain rate taper coefficient, vx is fluid velocity at distance x, wx is channel width at distance x, and εx is strain rate at distance x. 15. A device for stretching at least one polymer in a fluid sample, said device comprising: an elongation structure, wherein said elongation structure comprises a tapered channel, said tapered channel decreasing in width from a first end to a second end, said tapered channel comprising: a first zone having a first tapered shape;a second zone having a second tapered shape, wherein the second tapered shape is different than the first tapered shape; andwherein said at least one polymer, when present, moves along said tapered channel from said first end to said second end and is stretched,wherein the first tapered shape includes an increasing strain rate taper, andwherein a width w(x) of the tapered channel in the first zone is defined by the following equations: w(x)=2wiviax2=F1x2F1=2vxwxxɛ.xwherein x is distance along the channel, wi is channel width at arbitrary position i, vi is fluid velocity at arbitrary position i, F1 is a geometrical taper coefficient for an increasing strain rate funnel, vx is fluid velocity at distance x, wx is channel width at distance x, and {dot over (ε)}x is strain rate at distance x. 16. The device of claim 15, wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=w21+xaa=l2w2w3-1wherein l2 is the length of the second zone, w2 is the width of the tapered channel at a transition which separates the first zone from the second zone, and w3 is the width of the tapered channel at the second end of the tapered channel. 17. The device of claim 15, wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=F2/x F2=vxwx/{dot over (ε)}wherein x is distance along the tapered channel, F2 is a constant strain rate taper coefficient, vx is fluid velocity at distance x, wx is channel width at distance x, and εx is strain rate at distance x. 18. A device for stretching at least one polymer in a fluid sample, said device comprising: an elongation structure, wherein said elongation structure comprises a tapered channel, said tapered channel having a width w(x) which decreases from a first end to a second end, the tapered channel comprising: a first zone having a first shape;a second zone having a second shape, wherein the second shape is different than the first shape; anda transition which separates the first zone from the second zone;wherein the width w(x) of the tapered channel in the first zone is defined by the following equations: w(x)=1(bx+c)2b=1l1(1w2-1w1)c=1w1wherein l1 is the length of the first zone, w1 is the width of the tapered channel at the first end of the tapered channel, and w2 is the width of the tapered channel at the transition;wherein the width w(x) of the tapered channel in the second zone is defined by the following equations: w(x)=w21+xaa=l2w2w3-1and wherein l2 is the length of the second zone, w2 is the width of the tapered channel at the transition, and w3 is the width of the tapered channel at the second end of the tapered channel. 19. The device of claim 18, wherein the elongation structure is formed on a chip. 20. The device of claim 18, further comprising a delivery region for delivering said at least one polymer in said fluid sample to said elongation structure. 21. The device of claim 20, wherein said delivery region comprises a sample loading port and a delivery channel, said delivery channel leading into the elongation structure. 22. The device of claim 21, further comprising at least one buffer channel leading into the elongation structure. 23. The device of claim 22, wherein the at least one buffer channel includes two opposing buffer channels leading into the elongation structure.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (86)
Yager Paul ; Brody James P., Absorption-enhanced differential extraction device.
Mathies Richard A. (Contra Costa County CA) Huang Xiaohua C. (Santa Clara County CA) Quesada Mark A. (San Francisco County CA), Capillary array confocal fluorescence scanner and method.
Cheng Jing ; Sheldon ; III Edward L. ; Wu Lei ; O'Connell James P., Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis.
Baldarelli Richard ; Branton Daniel ; Church George ; Deamer David W. ; Akeson Mark ; Kasianowicz John, Characterization of individual polymer molecules based on monomer-interface interactions.
Church George ; Deamer David W. ; Branton Daniel ; Baldarelli Richard ; Kasianowicz John, Characterization of individual polymer molecules based on monomer-interface interactions.
Cathey Cheryl A. ; Saul Tom ; Bloom Nicole D. ; Ribi Hans O. ; Schwartz Henry L. ; Langford Jeffrey B. ; Paul David J., Device for use in analyte detection assays.
Mian Alec ; Kieffer-Higgins Stephen G. ; Corey George D., Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system.
Mathies Richard A. ; Glazer Alexander N. ; Lao Kaiqin ; Woolley Adam T., Electrochemical detector integrated on microfabricated capillary electrophoresis chips.
Maley Thomas C. (Medway MA) D\Orazio Paul A. (Mendon MA) Dalzell Bonnie C. (Sherborn MA) Edelman Peter G. (Franklin MA) Flaherty James E. (Attleboro MA) Mason Richard W. (Millis MA) McCaffrey Robert , Electrochemical sensors.
Maley Thomas C. ; D'Orazio Paul A. ; Dalzell Bonnie C. ; Edelman Peter G. ; Flaherty James E. ; Mason Richard W. ; McCaffrey Robert R., Electrochemical sensors membrane.
Wada, H. Garrett; Kopf-Sill, Anne R.; Alajoki, Marja Liisa; Parce, J. Wallace; Wang, Benjamin N.; Chow, Andrea W.; Dubrow, Robert S., Focusing of microparticles in microfluidic systems.
Mathies Richard A. (Contra Costa County CA) Peck Konan (Contra Costa County CA) Stryer Lubert (Santa Clara County CA), High sensitivity fluorescent single particle and single molecule detection apparatus and method.
Bensimon David,FRX ; Bensimon Aaron,FRX ; Heslot Fran.cedilla.ois,FRX, Highly specific surface for biological reactions having an exposed ethylenic double bond, process of using the surface,.
Becker, Frederick F.; Gascoyne, Peter R. C.; Huang, Ying; Wang, Xiao-Bo; Yang, Jun, Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation.
Ramsey, J. Michael; Foote, Robert S., Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid probes.
Fourmentin-Guilbert Jean E. R. (84 ; avenue de la Republique 93160 Noisy Le Grand FRX), Method for arranging a polynucleotide on a substrate for point-by-point analysis of the bases thereof.
Zhao,Xiaojian (David); Randall,Jeffrey D.; Kundu,Bijit; Kesty,Jessica; Gullans,Steven R.; Chan,Eugene Y.; Fuchs,Martin; Rooke,Jenny E., Methods and apparati using single polymer analysis.
Jacobson Stephen C. ; Ramsey J. Michael, Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same.
Austin Robert H. (Princeton NJ) Volkmuth Wayne D. (Menlo Park MN) Rathburn Lynn C. (Ithaca NY), Microlithographic array for macromolecule and cell fractionation.
Giddings John C. (Salt Lake City UT), Pinched channel inlet system for reduced relaxation effects and stopless flow injection in field-flow fractionation.
Bensimon David,FRX ; Bensimon Aaron,FRX ; Heslot Fran.cedilla.ois,FRX, Process for aligning, adhering and stretching nucleic acid strands on a support surface by passage through a meniscus.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.