The present invention provides methods of improving the osteogenic and/or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The tr
The present invention provides methods of improving the osteogenic and/or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The treatment or condition may alter the structure of the bone matrix and/or cleave one or more specific proteins. Cleavage may generate peptides or protein fragments that have osteoinductive, osteogenic, or chondrogenic activity. Preferred treatments include collagenase and various other proteases. The invention further provides improved bone and cartilage matrix compositions that have been prepared according to the inventive methods and methods of treatment using the compositions. The invention further provides methods of preparing, testing, and using the improved bone matrix compositions. On a assay comprises exposing relatively undifferentiated mesenchymal cells to a bone matrix composition and measuring expression of a marker characteristic of osteoblast or chondrocyte lineage(s). Increased expression of the marker relative to the level of the marker in cells that have been exposed to a control matrix (e.g., an inactivated or untreated matrix) indicates that the treatment or condition increased the osteogenic and/or chondrogenic activity of the bone matrix. Suitable cells include C2C12 cells. A suitable marker is alkaline phosphatase. The inventive methods increase the osteogenic and/or chondrogenic activity of human DBM when tested using this assay system.
대표청구항▼
1. A method of preparing a modified bone matrix, the method comprising: exposing a bone matrix to heat treatment to increase at least one biological activity and solubility of the bone matrix; andexogenously adding to the bone matrix a biodegradable diffusion barrier material;wherein the solubility
1. A method of preparing a modified bone matrix, the method comprising: exposing a bone matrix to heat treatment to increase at least one biological activity and solubility of the bone matrix; andexogenously adding to the bone matrix a biodegradable diffusion barrier material;wherein the solubility of the heat treated bone matrix is between 10% to 4000% greater than that of a bone matrix that is not subjected to heat treatment; andwherein the biodegradable diffusion barrier is dextran. 2. The method of claim 1, wherein the bone matrix is demineralized bone matrix derived at least in part from human bone, and the modified bone matrix comprises entangled bone fibers. 3. The method of claim 1, wherein the bone matrix comprises mineralized bone matrix, partially demineralized bone matrix, demineralized bone matrix, deorganified bone matrix, anorganic matrix, or a mixture thereof. 4. The method of claim 1, wherein the bone matrix comprises a mineralized, partially demineralized, demineralized, deorganified, or anorganic bone section. 5. The method of claim 1, wherein the biological activity is selected from the group consisting of: osteoinductive activity, osteogenic activity, chondrogenic activity, wound healing activity, neurogenic activity, contraction-inducing activity, mitosis-inducing activity, differentiation-inducing activity, chemotactic activity, angiogenic activity, vasculogenic activity, exocytosis-inducing activity, and endocytosis-inducing activity. 6. The method of claim 1, wherein the biodegradable diffusion barrier further comprises a second diffusion barrier material which has a degradation time which is different than the degradation time of dextran. 7. The method of claim 1, wherein the biodegradable diffusion barrier material is regionally degraded. 8. The method of claim 1, further comprising a step of adding to the bone matrix one or more bioactive agents selected from the group consisting of small molecules, chemical compounds, cells, polynucleotides, proteins, peptides, drugs, viruses, antibiotics, anti-neoplastic agents, growth factors, hematopoietic factors, hormones, wound healing factors, and nutrients. 9. A method of producing a device for bone repair comprising the step of: providing a bone matrix prepared according to the method of claim 1, optionally including one or more additional components; and forming the device into a shape suitable for implantation into a subject, thereby producing an implantable device.
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Merboth,Barbara L.; Sunwoo,Moon Hae; Gertzman,Arthur A., Allograft bone composition having a gelatin binder.
Collombel Christian (Lyon FRX) Damour Odile (Saint Genis Laval FRX) Gagnieu Christian (Lyon FRX) Poinsignon Frederique (Lyon FRX) Echinard Christian (Gemenos FRX) Marichy Jacques (Lyon FRX), Biomaterials with a base of mixtures of collagen, chitosan and glycosaminoglycans, process for preparing them and their.
Drohan William N. ; MacPhee Martin J. ; Miekka Shirley I. ; Singh Manish S. ; Elson Clive,CAX ; Taylor ; Jr. John R., Chitin hydrogels, methods of their production and use.
Kemp Paul D. (Winchester MA) Carr ; Jr. Robert M. (Boston MA) Maresh John G. (Somerville MA) Cavallaro John (Gloucester MA) Gross Jerome (Waban MA), Collagen threads.
Prewett Annamarie B. (Little Silver NJ) Stikeleather Roger C. (Doylestown PA), Demineralized bone particles and flowable osteogenic composition containing same.
Reiss,Paul; Ico,Cesar; Talmadge,Karen D; Reiley,Mark A; Scholten,Arie, Devices and methods using an expandable body with internal restraint for compressing cancellous bone.
Yim Kalvin W. K. (N. andover MA) Huberty Michael C. (Andover MA) Northey ; Jr. Richard P. (Ipswich MA) Schrier Jay A. (Andover MA), Formulations for delivery of osteogenic proteins.
John M. Winterbottom ; Lawrence Shimp ; Todd M. Boyce ; David Kaes, Implant, method of making same and use of the implant for the treatment of bone defects.
Stevens,Sheila S; Boucher,Ryan P; Follmer,Lulu; Salom,Nenita; Reiss,Paul; Ico,Cesar; Talmadge,Karen D; Reiley,Mark A; Scholten,Arie, Inflatable device for use in surgical protocol relating to fixation of bone.
Williams, Simon F.; Martin, David P.; Gerngross, Tillman; Horowitz, Daniel M., Medical device containing polyhydroxyalkanoate treated with oxidizing agent to remove endotoxin.
Hochschuler, Stephen; Johnson, Wesley; Nickels, Kevin L.; Hektner, Thomas R.; Wales, Larry; Lipschultz, Tyler, Method and apparatus for treating a vertebral body.
Lerch Alain,FRX ; Frayssinet Patrick,FRX, Method for preparing an implantable composite material, resulting material, implant including said material, and kit therefor.
Wallace Donald G. (Menlo Park CA) Smestad Thomas L. (Palo Alto CA) McPherson John M. (Sunnyvale CA) Piez Karl A. (Menlo Park CA) Seyedin Saeid (Sunnyvale CA) Armstrong Rosa (Palo Alto CA), Methods of bone repair using collagen.
Celeste Anthony J. (Hudson MA) Wozney John M. (Hudson MA) Rosen Vicki A. (Brookline MA) Wolfman Neil M. (Dover MA) Thomsen Gerald H. (Port Jefferson NY) Melton Douglas A. (Lexington MA), Methods of inducting formation of tendon and/or ligament tissue comprising administering BMP-12, BMP-13, and/or MP-52.
Vacanti Joseph P. (Winchester MA) Vacanti Charles A. (Lexington MA) Langer Robert S. (Newton MA), Neomorphogenesis of cartilage in vivo from cell culture.
Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (Hopkinton MA) Pang Roy H. L. (Medway MA), Osteogenic devices.
Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (Hopkinton MA) Pang Roy H. L. (Medway MA), Osteogenic proteins.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Concord CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Davis CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Davis CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone.
Seyedin Saeid (Sunnyvale CA) Thomas Thomas (Concord CA) Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Polypeptide cartilage-inducing factors found in bone used in tissue proliferation.
Scarborough Nelson L. ; Morris John W. ; Russell James L. ; Dowd Michael, Pressure flow system and method for treating a fluid permeable workpiece such as a bone.
Michael Dowd ; Nelson L. Scarborough ; Mark Daugherty ; Jack McMickle, Process for producing flexible sheets from demineralized, elongate, bone particles.
Fages Jacques,FRX ; Marty Alain,FRX ; Combes Didier,FRX ; Condoret Jean-Stephane,FRX, Process for treating bone tissue and corresponding implantable biomaterials.
Fages Jacques,FRX ; Marty Alain,FRX ; Combes Didier,FRX ; Condoret Jean-Stephane,FRX, Process for treating bone tissue and corresponding implantable biomaterials.
Thiele Heinrich (Neue Universitaet Kiel DEX) Thiele Erhard (Reventlow-Allee 9 23 Kiel DEX), Process of degrading and regenerating bone and tooth material and products.
Williams Simon F. ; Martin David P. ; Gerngross Tillman ; Horowitz Daniel M., Removing endotoxin with an oxdizing agent from polyhydroxyalkanoates produced by fermentation.
MacPhee Martin James ; Drohan William Nash ; Lasa ; Jr. Carlos I.,PHX ; Liau Gene ; Haudenschild Christian, Supplemented and unsupplemented tissue sealants, methods of their production and use.
Bogdansky Simon (Marlboro NJ) O\Leary Robert K. (Spring Lake NJ), Swollen demineralized bone particles, flowable osteogenic composition containing same and use of the composition in the.
Binette, Francois; Bowman, Steven M.; Bruker, Izi; Hwang, Julia; Melican, Mora Carolynne; Rezania, Alireza, Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration.
Castor Trevor P. ; Lander Arthur D. ; Cosman Maury David ; D'Entremont Peter Richard ; Pelletier Michael Richard, Viral inactivation method using near critical, supercritical or critical fluids.
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