Methods and compositions for identifying a cell phenotype
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/02
C12N-005/06
C12Q-001/04
G01N-033/574
출원번호
US-0673643
(2012-11-09)
등록번호
US-9057092
(2015-06-16)
발명자
/ 주소
Davis, Noa
Elkeles, Adi
Terkieltaub, Dov
Chausovsky, Alexander
출원인 / 주소
ZETIQ TECHNOLOGIES LTD.
대리인 / 주소
Rothwell, Figg, Ernst & Manbeck, P.C.
인용정보
피인용 횟수 :
0인용 특허 :
20
초록
The present invention is directed to a method of identifying cancerous or precancerous cells suspended in a liquid carrier, such as, a biological fluid.
대표청구항▼
1. A method of identifying at least one precancerous or cancerous cell present in a cell suspension, the method comprising: (a) providing a sample comprising a plurality of cells suspended in a liquid carrier;(b) fixing the plurality of cells onto a solid substrate;(c) contacting said plurality of c
1. A method of identifying at least one precancerous or cancerous cell present in a cell suspension, the method comprising: (a) providing a sample comprising a plurality of cells suspended in a liquid carrier;(b) fixing the plurality of cells onto a solid substrate;(c) contacting said plurality of cells with a Ficus plant extract;(d) contacting said plurality of cells with at least one dye; and(e) analyzing said plurality of cells,wherein when: (i) said at least one dye is an acidic dye, a staining intensity of at least one cell above a predetermined threshold is indicative that said at least one cell is a differentiated cell; (ii) said at least one dye is a basic dye, a staining intensity of at least one cell above a predetermined threshold is indicative that said at least one cell is an undifferentiated cell; (iii) said at least one dye comprises said acidic dye and said basic dye, a staining intensity of at least one cell above a predetermined threshold with said acidic dye is indicative that said at least one cell is a differentiated cell and a staining intensity of at least one cell above a predetermined threshold with said basic dye is indicative that said at least one cell is an undifferentiated cell. 2. The method of claim 1, wherein said at least one dye is a basic dye or an acidic dye. 3. The method of claim 2, wherein said basic dye is selected from the group consisting of Dahlia and new Fuchsin. 4. The method of claim 2, wherein said acidic dye is selected from the group consisting of Fast Green and Light Green. 5. The method of claim 1, wherein said at least one dye is a single dye or comprises two dyes. 6. The method of claim 1, wherein said Ficus plant is Ficus elastica. 7. The method of claim 1, wherein said Ficus plant extract is an ethanol extract of a leaf tissue. 8. The method of claim 1, wherein contacting said plurality of cells with a Ficus plant extract is carried out after contacting said plurality of cells with at least one dye. 9. The method of claim 1, wherein said sample is selected from the group consisting of a blood sample, a urine sample, a saliva sample, a cerebrospinal sample, an ascitic fluid sample and a semen sample. 10. The method of claim 1, wherein said liquid carrier is a biological fluid selected from the group consisting of: blood, plasma, saliva, semen, urine, cerebrospinal fluid, ascetic fluid and combinations thereof. 11. The method of claim 1, wherein said at least one undifferentiated cell is a malignant or pre-malignant cell. 12. A method of diagnosing cancer in a subject in need thereof, the method comprising: (a) obtaining from said subject a sample comprising a plurality of cells suspended in a biological fluid;(b) fixing the plurality of cells onto a solid substrate;(c) contacting said plurality of cells with a Ficus plant extract;(d) contacting said plurality of cells with at least one dye; and(e) analyzing said plurality of cells,wherein when: (i) said at least one dye is an acidic dye a staining intensity of at least one cell above a predetermined threshold is indicative that said at least one cell is a differentiated cell; (ii) said at least one dye is a basic dye a staining intensity of at least one cell above a predetermined threshold is indicative that said at least one cell is an undifferentiated cell; (iii) said at least one dye comprises said acidic dye and said basic dye a staining intensity of at least one cell above a predetermined threshold with said acidic dye is indicative that said at least one cell is a differentiated cell and a staining intensity of at least one cell above a predetermined threshold with said basic dye is indicative that said at least one cell is an undifferentiated cell. 13. The method of claim 12, wherein said at least one dye is a basic dye, or an acidic dye. 14. The method of claim 13, wherein said basic dye is selected from the group consisting of Dahlia and new Fuchsin. 15. The method of claim 13, wherein said acidic dye is selected from the group consisting of Fast Green and Light Green. 16. The method of claim 12, wherein said at least one dye is a single dye or comprises two dyes. 17. The method of claim 12, wherein said Ficus plant is Ficus elastica. 18. The method of claim 12, wherein said Ficus plant extract is an ethanol extract of a leaf tissue extract. 19. The method of claim 12, wherein said sample is selected from the group consisting of a blood sample, a urine sample, a saliva sample, a cerebrospinal sample, an ascitic fluid sample and semen sample. 20. The method of claim 12, wherein said at least one undifferentiated cell is a malignant or pre-malignant cell.
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