[특허]Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing

Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-001/28 G01N-001/40
출원번호	US-0856182 (2010-08-13)
등록번호	US-9103757 (2015-08-11)
발명자 / 주소	Baer, Thomas M. Reamey, Robert H. Moravick, Keith E. Head, David F. Richardson, Bruce J. Richardson, Derrick A.
출원인 / 주소	LIFE TECHNOLOGIES CORPORATION
인용정보	피인용 횟수 : 0 인용 특허 : 95

초록 ▼

The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.

대표청구항 ▼

1. A method for extracting biomolecules from a portion of cells from a cell sample on a carrier, wherein the cell sample includes a plurality of cells, the method comprising: contacting a stand-off portion of the carrier to a substrate, wherein the stand-off portion is configured to maintain a distance between the substrate and a transfer film, wherein a cell sample is included on the substrate;transferring a portion of cells selected for analysis from the cell sample included on the substrate to the transfer film, wherein the transfer film is included on the carrier;mating the carrier to an extraction device, wherein the extraction device is configured to remove biomolecules from the portion of cells selected for analysis, wherein the extraction device comprises: a carrier-receiving portion comprising a plurality of flanges, wherein the carrier-receiving portion comprises an inner surface, wherein the carrier-receiving portion further includes a landing portion having a landing surface, the landing surface defining an inner opening, anda conduit interconnected to the carrier-receiving portion, the conduit extending between a first opening on the carrier-receiving portion and a second opening at a second end, wherein the carrier-receiving portion is adapted to receive the carrier and to form a reservoir by the carrier contacting the landing portion, wherein the carrier mating with the carrier-receiving portion closes the inner opening to seal the inner opening to prevent fluid flow through a bottom surface;deflecting the flanges by passing a at least a portion of the carrier between the flanges so as secure the carrier to the extraction device in a compression-fit engagement;forming the reservoir with the transfer film by contacting the stand-off portion to the landing surface, wherein the stand-off portion and landing surface are configured so that the stand-off portion is not included in the reservoir;providing fluid into the reservoir to extract biomolecules from the portion of cells from the transfer film; andremoving the fluid from the reservoir. 2. The method of claim 1 wherein transferring a portion of cells selected for analysis from the cell sample to the transfer film includes transferring the portion of cells to the transfer film by specific transfer microcapture; and the method further includes disposing the portion of cells that is adhered to the transfer film by specific transfer microcapture within the reservoir. 3. The method of claim 1 wherein transferring the portion of cells selected for analysis to the transfer film includes transferring the portion of cells selected for analysis to the transfer film by non-specific transfer micro-capture; and the method further includes substantially excluding matter that is adhered to the transfer film by non-specific transfer microcapture from the reservoir. 4. The method of claim 1 further comprising covering the stand-off portion. 5. The method of claim 2, wherein contacting the stand-off portion of the carrier to the substrate includes a non-specific transfer of matter to the stand-off portion. 6. The method of claim 1, wherein the landing portion reduces the extraction of matter from the stand-off portion of the carrier that contacted the substrate.

이 특허에 인용된 특허 (95)

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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연합인증

Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing 원문보기

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Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

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