High throughput system for isolation, growth, and detection of lipid inclusions in bacteria
원문보기
IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0928312
(2010-12-07)
|
등록번호 |
US-9234229
(2016-01-12)
|
발명자
/ 주소 |
- Sundstrom, Eric R.
- Criddle, Craig S.
|
출원인 / 주소 |
- The Board of Trustees of the Leland Stanford Junior University
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
0 인용 특허 :
3 |
초록
▼
A method of high throughput growth and quantitative analysis of microorganisms is provided that includes providing a microtiter plate growth and gas delivery system having well plates disposed for growth of the microorganisms, and providing a spectroscopic screening system disposed to analyze lipid
A method of high throughput growth and quantitative analysis of microorganisms is provided that includes providing a microtiter plate growth and gas delivery system having well plates disposed for growth of the microorganisms, and providing a spectroscopic screening system disposed to analyze lipid inclusions of the microorganisms.
대표청구항
▼
1. A method of providing a high throughput growth and quantitative analysis of microorganisms, comprising: a. screening a throughput of cultures for bioplastic enrichment from an array of lipid producing bacteria using a microtiter plate growth and gas delivery system, wherein said microtiter plate
1. A method of providing a high throughput growth and quantitative analysis of microorganisms, comprising: a. screening a throughput of cultures for bioplastic enrichment from an array of lipid producing bacteria using a microtiter plate growth and gas delivery system, wherein said microtiter plate growth and gas delivery system comprises well plates disposed for growth of microorganisms and a spectroscopic screening system disposed for analyzing said bioplastic and a gas delivery system comprising controlled gas flow of methane, nitrogen, oxygen, carbon dioxide, carbon monoxide, hydrogen, ethane, propane, or other gaseous hydrocarbons as a continuous gradient along the microtiter plate;b. isolating lipid producing bacteria of said bioplastic;c. inducing production of bioplastic;d. determining a growth condition for enriching bioplastic production using said spectroscopic screening of said microtiter plate growth and gas delivery system. 2. The method according to claim 1, wherein said spectroscopic screening system comprises a fluorescent screening system. 3. The method according to claim 2, wherein said fluorescent screening comprises lipophilic fluorescent stains. 4. The method according to claim 3, wherein said lipophilic fluorescent stain comprises Nile Red. 5. The method according to claim 2, wherein said fluorescent screening comprises a flow cytometry screening system. 6. The method according to claim 2, wherein said fluorescent screening comprises a plate reader screening system. 7. The method according to claim 1, wherein said microorganisms are selected from the group consisting of bacteria, eukaryota, and archaea. 8. The method according to claim 7, wherein said bacteria comprises methanotrophic. 9. The method according to claim 1, wherein said microorganisms are grown in a pure culture. 10. The method according to claim 1, wherein said microorganisms are grown in a mixed culture. 11. The method according to claim 10, wherein mixed culture contains at least two known pure cultures. 12. The method according to claim 1, wherein said microorganisms are grown in an enrichment culture. 13. The method according to claim 12, wherein said enrichment culture contains an unknown consortium of microorganisms. 14. The method according to claim 13, wherein the source of said microorganisms selected from the group consisting of soil, activated sludge, and other natural environments. 15. The method according to claim 1, wherein said concentration gas gradient of gas constituents comprises a controlled gas mixture that is delivered to said plates. 16. The method according to claim 1, wherein said lipid inclusions comprise biological polyesters. 17. The method according to claim 16, wherein said biological polyesters comprise polyhydroxyalkanoates (PHAs). 18. The method according to claim 17, wherein said PHAs comprise polyhydroxybutyrate (PHB). 19. The method according to claim 1, wherein said microtiter plate growth and gas delivery system comprises a light source disposed to culture photosynthetic organisms.
이 특허에 인용된 특허 (3)
-
Delente Jacques J. (Kensington MD) Behrens Paul W. (Ellicott City MD) Hoeksema Scot D. (Elkridge MD), Closed photobioreactor and method of use.
-
Herrema, Markus; Kimmel, Kenton, Method for the production of polyhydroxyalkanoic acid.
-
Herrema,Markus Donald, Process for the utilization of ruminant animal methane emissions.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.