Phycotoxins are purified from a mixture of phycotoxins produced in a continuous process. Cyanobacteria are produced in a continuous culture, then lyzed, the cells pelleted and extracted, and the extract purified using an organic solvent-aqueous mixture and repeated passage through a diatomaceous ear
Phycotoxins are purified from a mixture of phycotoxins produced in a continuous process. Cyanobacteria are produced in a continuous culture, then lyzed, the cells pelleted and extracted, and the extract purified using an organic solvent-aqueous mixture and repeated passage through a diatomaceous earth column. The column is washed with acetic acid, then the neosaxitoxin extracted with an alcohol-water mixture. The eluate is passed through activated charcoal columns, which are washed with distilled water to remove the retained pigments and impurities, the further purified by HPLC. In one embodiment, the process produces only neosaxitoxin and saxitoxin. In another embodiment, the process produces only GTX2/3.
대표청구항▼
1. A method for the isolation and purification of phycotoxins produced by cyanobacteria, the method comprising a. Lysing the cyanobacteria cells of sonication to obtain a lysate,b. isolating the liquid component of the lysate by centrifugation,c. passing the liquid lysate through size exclusion gel
1. A method for the isolation and purification of phycotoxins produced by cyanobacteria, the method comprising a. Lysing the cyanobacteria cells of sonication to obtain a lysate,b. isolating the liquid component of the lysate by centrifugation,c. passing the liquid lysate through size exclusion gel filtration and collecting the eluted fractions,d. identifying and isolating eluted fractions containing a phycotoxins,e. passing the isolated fractions through cation-exchange chromatography, and collecting the eluted fractions,f. identifying and isolating eluted fractions containing the purified phycotoxins extracted from the lysed cyanobacteria,wherein the phycotoxin in the extract is selected from the group consisting of neosaxitoxin, saxitoxin, gonyaulatoxin 2 and gonyaulatoxin 3. 2. The method of claim 1, wherein the cyanobacteria is selected from the genera Cylindrospermopsis sp, Anabaena sp, Gomophosphaeria sp, Oscillatoria sp, Aphanizomenon sp, Lyngbya sp, and combinations thereof. 3. The method of claim 2, wherein the species is selected from the group consisting of Cylindrospermopsis raciborskii, Anphanizomenon flos-aquae, Aphanizomenon (APh) issatschenkoi (usaceb) Proskina-Lavrenco, Aphanizomenon gracile (Lemm) Lemm, Anabaena circinalis, Lyngbya wollei, and combinations thereof. 4. The method of claim 1, wherein the cyanobacterium is Aphanizomenon gracile (Lemm) Lemm. 5. The method of claim 1, wherein the extract contains less than 13% by weight of saxitoxin. 6. The method of claim 1, wherein the extract contains at least 98% pure neosaxitoxin. 7. The method of claim 6, wherein the structure of the neosaxitoxin is 8. The method of claim 1, wherein the extract contains at least 98% pure saxitoxin. 9. The method of claim 8, wherein the structure of the saxitoxin is
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (35)
Berde Charles B. (Brookline MA) Langer Robert S. (Newton MA), Biodegradable polymer matrices for sustained delivery of local anesthetic agents.
Berman Elizabeth F. (Cincinnati OH) Buckwalter Brian L. (Yardley PA) Cupps Thomas L. (Oxford OH) Gardner Joseph H. (Cincinnati OH), Compounds and compositions having anti-inflammatory and analgesic activity.
Gardner Joseph H. (Cincinnati OH) Kasting Gerald B. (Wyoming OH) Cupps Thomas L. (Oxford OH) Echler Richard S. (Fairfield OH) Gibson Thomas W. (Cincinnati OH) Shulman Joel I. (Cincinnati OH), Novel compounds, pharmaceutical compositions, and methods for treating inflammation and pain.
Gardner Joseph H. (Cincinnati OH) Kasting Gerald B. (Wyoming OH) Cupps Thomas L. (Oxford OH) Echler Richard S. (Fairfield OH) Gibson Thomas W. (Cincinnati OH) Shulman Joel I. (Cincinnati OH), Novel compounds, pharmaceutical compositions, and methods for treating inflammation and pain.
Patel Dinesh C. (Murray UT) Chang Yunik (Lakewood NJ), Penetration enhancement with binary system of oleic acid, oleins, and oleyl alcohol with lower alcohols.
Sanders Harold F. (Palo Alto CA) Cheng Yu-Ling (Cupertino CA) Enscore David J. (Saratoga CA) Libicki Shari B. (Palo Alto CA), Transdermal drug composition with dual permeation enhancers.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.