Cellular arrays and methods of detecting and using genetic disorder markers
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/04
C12Q-001/68
B01L-003/00
G01N-001/28
G01N-001/31
G01N-033/50
G01N-033/543
G02B-021/34
G01N-001/08
G01N-001/36
출원번호
US-0676382
(2012-11-14)
등록번호
US-9284610
(2016-03-15)
발명자
/ 주소
Kallioniemi, Olli-P
Muller, Uwe Richard
Sauter, Guido
Kononen, Juha
Barlund, Maarit
출원인 / 주소
Abbott Molecular Inc.
대리인 / 주소
Pierce Atwood LLP
인용정보
피인용 횟수 :
0인용 특허 :
11
초록▼
A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological anal
A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristic of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue.
대표청구항▼
1. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human
1. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human genomic library and suitable for detecting expression of PDGF- βin the human breast cancer specimen; (ii) a labeled locus specific probe for chromosomal region 7q31; (iii) a labeled locus specific probe for chromosomal region 8p11-p12; and (iv) a labeled locus specific probe for chromosomal region 10q25-26, wherein probes (ii), (iii), and (iv) are suitable for detecting their target chromosomal regions in the human breast cancer specimen, and wherein probes (i), (ii), (iii), and (iv) are labeled with a fluorescent dye. 2. The composition of claim 1 used in a method to detect breast cancer, wherein probes (i), (ii), (iii), and (iv) are detected by polymerase chain reaction (PCR). 3. The composition of claim 1 used in a method to detect breast cancer, wherein probes (i), (ii), (iii), and (iv) are detected by fluorescence in situ hybridization (FISH). 4. The composition of claim 1 used in a method to detect breast cancer, wherein probes (i), (ii), (iii), and (iv) are detected using a cDNA microarray. 5. The composition of claim 1 wherein probes (i), (ii), (iii), and (iv) are each labeled with fluorescent dye of a unique emission wave length. 6. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human genomic library and suitable for detecting expression of PDGF-βin the human breast cancer specimen; (ii) a labeled locus specific probe for chromosomal region 7q31; and (iii) a labeled locus specific probe for chromosomal region 8p11-p12, wherein probes (ii) and (iii) are suitable for detecting their target chromosomal regions in the human breast cancer specimen, and wherein probes (i), (ii), and (iii) are labeled with a fluorescent dye. 7. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human genomic library and suitable for detecting expression of PDGF-βin the human breast cancer specimen; (ii) a labeled locus specific probe for chromosomal region 7q31; and (iii) a labeled locus specific probe for chromosomal region 10q25-26, wherein probes (ii) and (iii) are suitable for detecting their target chromosomal regions in the human breast cancer specimen, and wherein probes (i), (ii), and (iii) are labeled with a fluorescent dye.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (11)
Gray Joe W. ; Collins Colin ; Pinkel Daniel ; Kallioniemi Olli-Pekka,FIX ; Tanner Minna M.,FIX, Amplifications of chromosomal region 20q13 as a prognostic indicator in breast cancer.
Pinkel Daniel (Walnut Creek CA) Gray Joe W. (San Francisco CA) Kallioniemi Anne (Tampere FIX) Kallioniemi Olli-Pekka (Tampere FIX) Waldman Frederic (San Francisco CA), Comparative genomic hybridization (CGH).
Fishel Richard A. ; Gradia Scott ; Acharya Samir, Compositions, kits, and methods for effecting adenine nucleotide modulation of DNA mismatch recognition proteins.
Keay Susan K. ; Warren John W. ; Hise Michael K., Heparin binding--epidermal growth factor-like growth factor in the diagnosis of Interstitial Cystitis.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.