Incapacitated whole-cell immunogenic bacterial compositions produced by recombinant expression
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-039/02
A61P-037/04
C12N-015/74
A61K-039/108
A61K-039/00
출원번호
US-0560321
(2009-09-15)
등록번호
US-9289481
(2016-03-22)
발명자
/ 주소
Ramachandran, Janakiraman
Padmanabhan, Sriram
Sriram, Bharathi
출원인 / 주소
Gangagen, Inc.
대리인 / 주소
Kilpatrick Townsend & Stockton LLP
인용정보
피인용 횟수 :
0인용 특허 :
7
초록▼
The present invention features incapacitated whole-cell bacterial immunogenic compositions and methods of their production, which compositions are useful to deliver antigens in a manner resembling the live infectious organism in terms of elicitation of a robust immune response, but with reduced risk
The present invention features incapacitated whole-cell bacterial immunogenic compositions and methods of their production, which compositions are useful to deliver antigens in a manner resembling the live infectious organism in terms of elicitation of a robust immune response, but with reduced risk or no risk of disease. The compositions of the invention are produced by rendering a bacterium bacteriostatic through expression of a recombinant promoter in the bacterial cell, which promoter can be operably linked to a polynucleotide encoding a recombinant gene product. In one embodiment, where the bacterium is a gram negative host, the recombinant gene product provides for reduced toxicity of LPS. In one embodiment, the gene product is a bacteriophage protein, such as endolysin, holin, or ndd.
대표청구항▼
1. A method of immunizing a subject against a bacterial pathogen, the method comprising: administering to the subject an incapacitated immunogenic bacterial composition comprising the bacterial pathogen carrying an expression vector comprising a recombinant promoter operably linked to a polynucleoti
1. A method of immunizing a subject against a bacterial pathogen, the method comprising: administering to the subject an incapacitated immunogenic bacterial composition comprising the bacterial pathogen carrying an expression vector comprising a recombinant promoter operably linked to a polynucleotide encoding a gene product,wherein the recombinant promoter is a bacteriophage promoter, and wherein the bacterial pathogen is recombinantly modified to express a bacteriophage RNA polymerase for transcription from the bacteriophage promoter,wherein expression of the gene product is at a level sufficient to render the bacterial pathogen incapacitated, thereby producing said incapacitated immunogenic bacterial composition,wherein administration of said incapacitated immunogenic bacterial composition elicits an immune response to an antigen on the bacterial pathogen, thereby immunizing the subject against the bacterial pathogen, andwherein(i) the bacteriophage promoter is a T7 bacteriophage promoter and the bacteriophage RNA polymerase is T7 RNA polymerase,(ii) the bacteriophage promoter is a SP6 bacteriophage promoter and the bacteriophage RNA polymerase is SP6 RNA polymerase,(iii) the bacteriophage promoter is a T3 bacteriophage promoter and the bacteriophage RNA polymerase is T3 RNA polymerase, or(iv) the bacteriophage promoter is a T7 bacteriophage promoter and the bacteriophage RNA polymerase is T7 RNA polymerase. 2. The method of claim 1, wherein the bacterial pathogen is of a genus selected from the group consisting of Bordetella, Mycobacteria, Staphylococci, Vibrio, Enterobacter, Enterococcus, Escherichia, Haemophilus, Neisseria, Pseudomonas, Shigella, Serratia, Salmonella, Streptococcus, Klebsiella and Yersinia. 3. The method of claim 1, wherein the expression cassette is on a plasmid. 4. The method of claim 1, wherein the bacterial pathogen is Escherichia coli or Staphylococcus aureus.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (7)
Steidler, Lothar; Remaut, Erik; Wells, Jeremy Mark; Le Page, Richard William Falla, Delivery of biologically active polypeptides.
Kordyum, Vitaliy A.; Slavchenko, Iryna Yu.; Chernykh, Svitlana I.; Vozianov, Oleksandr F., Phage-dependent superproduction of biologically active protein and peptides.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.