Rare cell analysis using sample splitting and DNA tags
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
B01L-003/00
G01N-015/10
출원번호
US-0837974
(2013-03-15)
등록번호
US-9347100
(2016-05-24)
발명자
/ 주소
Shoemaker, Daniel
Kapur, Ravi
Toner, Mehmet
Stoughton, Roland
Davis, Ronald W.
출원인 / 주소
GPB Scientific, LLC
대리인 / 주소
Fish & Richardson P.C.
인용정보
피인용 횟수 :
0인용 특허 :
202
초록▼
The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixe
The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
대표청구항▼
1. A computer program product embodied in a non-transitory computer readable medium comprising instructions executable by one or more processors to cause a system to perform functions to determine a presence or absence of a fetal aneuploidy in a mixture of fetal and maternal genomic DNA obtained fro
1. A computer program product embodied in a non-transitory computer readable medium comprising instructions executable by one or more processors to cause a system to perform functions to determine a presence or absence of a fetal aneuploidy in a mixture of fetal and maternal genomic DNA obtained from a maternal blood sample obtained from a woman who is pregnant or who is suspected of being pregnant, wherein the functions comprise: receiving genomic DNA sequence data representing a complete genome, wherein the DNA sequence data is obtained by conducting whole genome amplification of a mixture of fetal and maternal genomic DNA to obtain amplified nucleic acid molecules, and conducting ultra-deep sequencing of the amplified nucleic acid molecules obtained from the mixture of fetal and maternal genomic DNA, thereby producing sequence data representing the complete genome, and wherein the ultra-deep sequencing comprises further amplification of the amplified nucleic acid molecules to produce at least one million copies of individual amplified nucleic acid molecules in parallel;processing the DNA sequence data to quantify DNA regions of (i) at least one test chromosome being tested for a presence or absence of fetal aneuploidy, and (ii) at least one control chromosome that is diploid;determining the presence or absence of fetal aneuploidy for the at least one test chromosome based on data for the quantified DNA regions of the at least one test chromosome and the at least one control chromosome;constructing an image file from the DNA sequence data andprinting or displaying an image from the data in the image file. 2. The computer program product of claim 1, wherein ultra-deep sequencing comprises sequencing-by-synthesis. 3. The computer program product of claim 2, wherein sequencing-by-synthesis involves synthesizing nucleic acid strands complementary to the amplified nucleic acid molecules and inferring nucleic acid sequences of the amplified nucleic acid molecules from the complementary synthesized nucleic acid strands. 4. The computer program product of claim 2, wherein sequencing-by-synthesis generates at least 1,000, at least 5,000, at least 10,000, at least 20,000, at least 30,000, at least 40,000, at least 50,000, at least 100,000, or at least 500,000 reads per hour. 5. The computer program product of claim 4, wherein the reads have at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 120, or at least 150 bases per read. 6. The computer program product of claim 1, wherein each of the amplified nucleic acid molecules generated by whole genome amplification comprises a tag. 7. The computer program product of claim 6, wherein the tag comprises a sequencing element. 8. The computer program product of claim 7, wherein the sequencing element is about 4, 6, 8, 10, 18, 20, 28, 36, 46, or 50 nucleotide bases in length. 9. The computer program product of claim 8, wherein the ultra-deep sequencing comprises sequencing-by-synthesis initiated using sequencing primers complementary to the sequencing element. 10. The computer program product of claim 9, wherein sequencing-by-synthesis comprises detecting an identity of each nucleotide immediately after or upon incorporation of a labeled nucleotide or nucleotide analog into a growing strand complementary to an amplified nucleic acid molecule. 11. The computer program product of claim 1, wherein ultra-deep sequencing comprises sequencing by ligation. 12. The computer program product of claim 11, wherein sequencing by ligation comprises a four-color sequencing by ligation. 13. The computer program product of claim 11, wherein sequencing by ligation comprises hybridizing an anchor primer to one of four positions on the amplified nucleic acid molecules. 14. The computer program product of claim 11, wherein sequencing by ligation comprises an enzymatic ligation reaction. 15. The computer program product of claim 11, wherein sequencing by ligation comprises four-color imaging. 16. The computer program product of claim 1, wherein the amplified nucleic acid molecules generated by whole genome amplification are mixed with beads such that a single amplified nucleic acid molecule attaches to each bead, and wherein ultra-deep sequencing comprises further amplification of the amplified nucleic acid molecule attached to each bead to produce at least one million copies of the single amplified nucleic acid molecule attached to each bead. 17. The computer program product of claim 16, wherein the amplified nucleic acid molecule attached to each bead is amplified by polymerase chain reaction (PCR). 18. The computer program product of claim 16, wherein the beads are placed into a highly parallel sequencing-by-synthesis machine that generates over 400,000 reads in a single 4 hour run. 19. The computer program product of claim 1, wherein the fetal aneuploidy comprises monosomy, trisomy, tetrasomy, or pentasomy of one or more chromosomes. 20. The computer program product of claim 19, wherein the one or more chromosomes are sex chromosomes. 21. The computer program product of claim 19, wherein the fetal aneuploidy comprises a trisomy. 22. The computer program product of claim 21, wherein trisomy comprises trisomy 13, trisomy 18, or trisomy 21. 23. The computer program product of claim 19, wherein monosomy comprises monosomy X. 24. The computer program product of claim 19, wherein the at least one chromosome being tested for aneuploidy is selected from the group consisting of chromosome 13, chromosome 18, chromosome 21, chromosome X, and chromosome Y. 25. The computer program product of claim 1, wherein the at least one chromosome being tested for aneuploidy is selected from the group consisting of chromosome 13, chromosome 18, chromosome 21, chromosome X, and chromosome Y. 26. The computer program product of claim 1, wherein the computer program comprises logic to fit data into one or more data models that provide for a determination of the presence or absence of a fetal aneuploidy. 27. The computer program product of claim 26, wherein the computer program receives input from a digital input device or a scanner.
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