Systems, methods, and media for providing a view of a digital slide image. In an embodiment, a digital slide image file is accessed. The digital slide image file may comprise a plurality of first image planes representing an image of at least a portion of a slide specimen at varying focal depths. Th
Systems, methods, and media for providing a view of a digital slide image. In an embodiment, a digital slide image file is accessed. The digital slide image file may comprise a plurality of first image planes representing an image of at least a portion of a slide specimen at varying focal depths. Then, a three-dimensional object is constructed from the digital slide image file. The three-dimensional image object is constructed by determining a surface that crosses two or more of the plurality of first image planes, and determining image data that are interior to the determined surface, based on one or more of the plurality of first image planes. A view is generated from the three-dimensional image object.
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1. A method for providing a view of a digital slide image, the method comprising using at least one hardware processor to: access at least one digital slide image file, wherein the at least one digital slide image file comprises a plurality of first image planes representing an image of at least a p
1. A method for providing a view of a digital slide image, the method comprising using at least one hardware processor to: access at least one digital slide image file, wherein the at least one digital slide image file comprises a plurality of first image planes representing an image of at least a portion of a slide specimen at varying focal depths;construct a three-dimensional image object from the at least one digital slide image file by determining a surface for the three-dimensional image object, wherein at least a portion of the surface crosses two or more of the plurality of first image planes, anddetermining image data that are interior to the determined surface, based on one or more of the plurality of first image planes; andgenerate a virtual slide view of the three-dimensional image object from the varying focal depths in virtual microscopy. 2. The method of claim 1, wherein determining a surface for the three-dimensional image object comprises calculating a surface for the three-dimensional image object based on an equation. 3. The method of claim 2, further comprising using the at least one hardware processor to receive a three-dimensional point within the plurality of first image planes, a height value, a width value, and a depth value, and wherein calculating a surface for the three-dimensional image object based on an equation comprises calculating a surface of a prism having the three-dimensional point as a corner of the surface, having a height defined by the height value, having a width defined by the width value, and having a depth defined by the depth value. 4. The method of claim 1, wherein determining image data that are interior to the determined surface comprises extracting at least a first portion of the image data from one or more of the plurality of first image planes, and interpolating at least a second portion of the image data based on one or more of the plurality of first image planes. 5. The method of claim 4, wherein the plurality of first image planes are spaced apart from each other along an axis representing a focal depth. 6. The method of claim 1, wherein the at least a portion of the surface is skewed across the two or more first image planes. 7. The method of claim 1, wherein the virtual slide view of the three-dimensional image object is two-dimensional. 8. The method of claim 1, further comprising using the at least one hardware processor to: receive a request from a user, wherein the request comprises a resolution; andscale the virtual slide view of the three-dimensional image object to the requested resolution. 9. The method of claim 1, wherein the virtual slide view of the three-dimensional image object is tilted with respect to an axis representing a focal depth of the plurality of first image planes. 10. The method of claim 1, further comprising using the at least one hardware processor to pre-render the three-dimensional image object at a plurality of resolutions. 11. The method of claim 1, wherein each of the plurality of first image planes comprises a plurality of image blocks. 12. The method of claim 1, wherein the at least one digital slide image file comprises each of the plurality of first image planes at a plurality of resolutions. 13. The method of claim 1, further comprising using the at least one hardware processor to rotate the virtual slide view of the three-dimensional image object. 14. The method of claim 1, wherein the virtual slide view of the three-dimensional image object is three-dimensional, and wherein the method further comprises using the at least one hardware processor to slice the three-dimensional view of the three-dimensional image object. 15. A system for providing a view of a digital slide image, the system comprising: at least one hardware processor;a memory having at least one digital slide image file stored therein, wherein the at least one digital slide image file comprises a plurality of first image planes representing an image of at least a portion of a slide specimen at varying focal depths; andone or more executable modules that, when executed by the at least one hardware processor, construct a three-dimensional image object from the at least one digital slide image file by determining a surface for the three-dimensional image object, wherein at least a portion of the surface crosses two or more of the plurality of first image planes, and determining image data that are interior to the determined surface, based on one or more of the plurality of first image planes, andgenerate a virtual slide view of the three-dimensional image object from the varying focal depths in virtual microscopy. 16. The system of claim 15, wherein determining a surface for the three-dimensional image object comprises calculating a surface for the three-dimensional image object based on an equation. 17. The system of claim 15, further comprising an image acquisition device that generates the digital slide image file by moving an objective lens through the varying focal depths with respect to the at least a portion of the slide specimen to acquire the plurality of first image planes. 18. A non-transitory computer-readable medium having instructions stored thereon that, when executed by a processor, cause the processor to: access at least one digital slide image file, wherein the at least one digital slide image file comprises a plurality of first image planes representing an image of at least a portion of a slide specimen at varying focal depths;construct a three-dimensional image object from the at least one digital slide image file by determining a surface for the three-dimensional image object, wherein at least a portion of the surface crosses two or more of the plurality of first image planes, anddetermining image data that are interior to the determined surface, based on one or more of the plurality of first image planes; andgenerate a virtual slide view of the three-dimensional image object from the varying focal depths in virtual microscopy. 19. The non-transitory computer-readable medium of claim 18, wherein determining a surface for the three-dimensional image object comprises calculating a surface for the three-dimensional image object based on an equation. 20. The non-transitory computer-readable medium of claim 19, wherein the at least a portion of the surface is skewed across the two or more first image planes.
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