Methods for producing 3-hydroxypropionic acid and other products
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12P-007/40
C12P-007/42
C12N-009/00
C12N-001/20
C12M-003/00
C07H-021/04
C12N-015/63
A61L-015/24
C12M-001/00
C12N-009/02
C12P-007/52
출원번호
US-0916534
(2013-06-12)
등록번호
US-9388419
(2016-07-12)
발명자
/ 주소
Lynch, Michael D.
Gill, Ryan T.
Lipscomb, Tanya E. W.
출원인 / 주소
Cargill, Incorporated
대리인 / 주소
Wilson Sonsini Goodrich & Rosati
인용정보
피인용 횟수 :
5인용 특허 :
65
초록
This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
대표청구항▼
1. A method for making acrylic acid, comprising: (a) producing 3-HP with a genetically modified microorganism comprising one or more heterologous nucleic acid sequences for increasing enzymatic activity in the malonyl-CoA reductase pathway and increasing enzymatic activity in the acetyl-CoA carboxyl
1. A method for making acrylic acid, comprising: (a) producing 3-HP with a genetically modified microorganism comprising one or more heterologous nucleic acid sequences for increasing enzymatic activity in the malonyl-CoA reductase pathway and increasing enzymatic activity in the acetyl-CoA carboxylase pathway of said microorganism; and(b) converting said 3-HP produced by said genetically modified microorganism to acrylic acid. 2. The method of claim 1, wherein one of said one or more heterologous nucleic acid sequences codes for a polypeptide comprising bi-functional malonyl-CoA reductase enzymatic activity. 3. The method of claim 1, wherein one of said one or more heterologous nucleic acid sequences codes for a polypeptide comprising mono-functional malonyl-CoA reductase activity. 4. The method of claim 3, wherein said mono-functional malonyl-CoA reductase enzymatic activity is NADPH-independent. 5. The method of claim 1, wherein one of said one or more heterologous nucleic acid sequences comprises at least 80% homology to a sequence selected from the group consisting of: SEQ ID NOs: 768-775, and 780-789. 6. The method of claim 1, wherein said genetically modified microorganism comprises a genetic modification resulting in reduced enzymatic activity in an enzyme selected from the group consisting of: enoyl-ACP reductase, lactate dehydrogenase, phosphate acetyltransferase, pyruvate oxidase, pyruvate-formate lyase, and acetate kinase, or any combination thereof. 7. The method of claim 1, wherein said genetically modified microorganism comprises a genetic modification resulting in reduced enzymatic activity in the fatty acid synthase pathway of said microorganism. 8. The method of claim 1, wherein said genetically modified microorganism is genetically modified to result in increased: tolerance to 3-hydoxypropionic acid (3-HP), enzymatic activity in the NADPH-dependent transhydrogenase pathway of said microorganism, or intracellular bicarbonate levels. 9. The method of claim 1, wherein said producing comprises culturing one or more of said genetically modified microorganism such that said 3-HP is produced at a volumetric productivity of at least 0.05 grams per liter per hour. 10. The method of claim 1, wherein said producing comprises culturing one or more of said genetically modified microorganism such that said 3-HP occurs at a productivity of at least 0.05 grams per gram of said genetically modified microorganism on a dry weight basis per hour. 11. The method of claim 1, wherein said genetically modified organism is an organism selected from the group consisting of: yeast, Lactobacillus, Lactococcus, Bacillus, and Escherichia. 12. The method of claim 1, wherein said genetically modified microorganism comprises a mutation in a gene selected from the group consisting of: aldA, aldB, and puuC. 13. The method of claim 1, further comprising isolating said 3-HP in the presence of a tertiary amine. 14. The method of claim 1, wherein said converting comprises dehydrating said 3-HP. 15. The method of claim 1, wherein said converting comprises contacting said 3-HP with a dehydration catalyst. 16. The method of claim 15, wherein said dehydration catalyst is an aluminum oxide. 17. The method of claim 1, wherein said enzymatic activity in said malonyl-CoA reductase pathway or said acetyl-CoA carboxylase pathway is increased by at least 20% compared to an unmodified microorganism. 18. The method of claim 1, wherein said enzymatic activity in said malonyl-CoA reductase pathway or said acetyl-CoA carboxylase pathway is increased by at least 50% compared to an unmodified microorganism. 19. The method of claim 1, wherein said genetically modified microorganism is genetically modified to reduce production of a chemical selected from the group consisting of: acetate, acetoin, acetone, malate, fatty acid ethyl esters, isoprenoid, glycerol, ethylene glycol, ethylene, acetates, butanols, ethanols, propanols, formate, fumaric acid, succinic acid, valeric acid and maleic acid.
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이 특허에 인용된 특허 (65)
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Lin,YuPo J.; Henry,Michael; Hestekin,Jamie; Snyder,Seth W.; St. Martin,Edward J., Single-stage separation and esterification of cation salt carboxylates using electrodeionization.
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