Separating target analytes using alternating magnetic fields
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/543
G01N-033/569
C12Q-001/68
A61B-005/055
G01R-033/465
G01N-024/08
C07K-001/22
C07K-016/12
C12N-013/00
출원번호
US-0855147
(2010-08-12)
등록번호
US-9389225
(2016-07-12)
발명자
/ 주소
Dryga, Sergey A.
Esch, Victor C.
Saul, Richard G.
McDowell, Andrew F.
출원인 / 주소
DNA Electronics, Inc.
대리인 / 주소
Meyers, Thomas C.
인용정보
피인용 횟수 :
3인용 특허 :
133
초록▼
The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby f
The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.
대표청구항▼
1. A method for separating and analyzing a target analyte in a sample, the method comprising: contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/partic
1. A method for separating and analyzing a target analyte in a sample, the method comprising: contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles;flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte;applying alternating magnetic fields to the flowing sample to result in the target/particle complexes being brought into proximity of the surface to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample;binding the target/particle complexes to the second moieties so as to form bound target/particle/second moiety complexes;flowing away unbound particles and unbound non-target components of the sample;eluting the bound target/particle/second moiety complexes from the surface of the channel; andanalyzing the target/particle/second moiety complexes only after the eluting step, wherein the target/particle/second moiety complexes are not examined in situ on the surface. 2. The method of claim 1, wherein analyzing comprises flowing the target/particle/second moiety complexes into an NMR instrument. 3. The method of claim 1, wherein the target analyte is selected from the group consisting of a bacteria, a virus, a fungus, a cell, a protein, and a nucleic acid. 4. The method of claim 3, wherein the target analyte is a bacterium. 5. The method of claim 1, wherein the sample is human tissue or body fluid. 6. The method of claim 5, wherein the body fluid is blood. 7. The method according to claim 1, wherein the first and second moieties are the same. 8. The method of claim 1, wherein the first and second moieties are different. 9. The method of claim 1, wherein the first and second moieties are selected from the group consisting of antibodies, receptors, aptamers, proteins, and ligands. 10. The method of claim 1, wherein the alternating magnetic fields result from the channel being positioned between first and second sets of magnets, wherein the channel remains stationary and the first and second sets of magnets are moved to alternate proximity to the channel, thereby producing the alternating magnetic fields. 11. The method according to claim 1, wherein the alternating magnetic fields result from the channel being positioned between first and second sets of magnets, wherein first and second sets of magnets remain stationary and the channel is moved to alternate its proximity to the first and second sets of magnets, thereby producing the alternating magnetic fields. 12. A method for detecting a target analyte in a sample, the method comprising: contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles;flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte;applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface of the channel to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample;binding the target/particle complexes to the second moieties so as to form bound target/particle/second moiety complexes;flowing away unbound particles and unbound non-target components of the sample;eluting the target/particle/second moiety complexes from the surface of the channel; anddetecting the target/particle/second moiety complexes only after the eluting step, and wherein the target/particle/second moiety complexes are not examined in situ on the surface. 13. The method of claim 12, wherein the alternating magnetic fields result from the channel being positioned between first and second sets of magnets, wherein the channel remains stationary and the first and second sets of magnets are moved to alternate proximity of the first and second sets of magnets to the channel, thereby producing the alternating magnetic fields. 14. The method according to claim 12, wherein the alternating magnetic fields result from the channel being positioned between first and second sets of magnets, wherein the first and second sets of magnets remain stationary and the channel is moved to alternate its proximity to the first and second sets of magnets, thereby producing the alternating magnetic fields. 15. The method of claim 12, wherein detection comprises flowing the target/particle/second moiety complexes into an NMR instrument. 16. The method of claim 12, wherein the target analyte is a bacterium. 17. The method of claim 12, wherein the sample is human tissue or body fluid. 18. The method of claim 17, wherein the body fluid is blood. 19. The method according to claim 12, wherein the first and second moieties are the same. 20. The method of claim 12, wherein the first and second moieties are different. 21. The method of claim 12, wherein the first and second moieties are selected from the group consisting of antibodies, receptors, aptamers, proteins, and ligands. 22. A method for separating and analyzing a target analyte in a sample, the method comprising: contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles;applying a magnetic field to capture target/particle complexes on a surface;washing the target/particle complexes;removing the magnetic field, thereby releasing target/particle complexes from the surface;flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte;applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface of the channel to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample;binding the target/particle complexes to the second moieties to form bound target/particle/second moiety complexes;flowing away unbound particles and unbound non-target components of the sample;eluting the bound target/particle/second moiety complexes from the surface of the channel by eluting the second moieties from the surface of the channel; andanalyzing the target/particle/second moiety complexes only after the eluting step, wherein the target/particle/second moiety complexes are not examined in situ on the surface.
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