Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-005/02
C12N-005/074
C12N-005/0735
C12N-005/00
C12N-005/0797
출원번호
US-0578443
(2014-12-21)
등록번호
US-9410121
(2016-08-09)
발명자
/ 주소
Amit, Michal
Itskovitz-Eldor, Joseph
출원인 / 주소
Technion Research & Development Foundation Limited
인용정보
피인용 횟수 :
1인용 특허 :
30
초록▼
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno con
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
대표청구항▼
1. A culture medium being devoid of serum and comprising bFGF, TGFβ1 and transferrin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages. 2. The culture medium of claim 1, further comprising insulin. 3. The culture medium o
1. A culture medium being devoid of serum and comprising bFGF, TGFβ1 and transferrin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages. 2. The culture medium of claim 1, further comprising insulin. 3. The culture medium of claim 1, wherein the culture medium is free of an animal contaminant. 4. The culture medium of claim 1, wherein the culture medium is free of feeder cell contaminants. 5. The culture medium of claim 1, wherein said TGFβ1 is provided at a concentration range of 0.06-0.24 ng/ml. 6. The culture medium of claim 1, wherein said bFGF is provided at a concentration of at least 2 ng/ml. 7. The culture medium of claim 1, wherein said bFGF is provided at a concentration rage of 2-8 ng/ml. 8. The culture medium of claim 2, wherein said TGFβ1 is provided at a concentration range of 0.06-0.24 ng/ml, and said bFGF is provided at a concentration of at least 2 ng/ml. 9. The culture medium of claim 1, further comprising leukemia inhibitor factor (LIP). 10. The culture medium of claim 9, wherein said LIF is provided at a concentration of at least 500 units/ml. 11. The culture medium of claim 9, wherein said LIF is provided at a concentration range of 500-2000 units/ml. 12. The culture medium of claim 1, wherein said medium is capable of maintaining the pluripotent stem cell in an undifferentiated state for at least 38 passages. 13. A culture medium being devoid of serum and comprising bFGF, TGFβ1 and insulin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state. 14. The culture medium of claim 13, further comprising transferrin. 15. The culture medium of claim 13, wherein the culture medium is free of an animal contaminant. 16. The culture medium of claim 13, wherein the culture medium is free of feeder cell contaminants. 17. The culture medium of claim 13, wherein said TGFβ1 is provided at a concentration range of 0.06-0.24 ng/ml. 18. The culture medium of claim 13, wherein said bFGF is provided at a concentration of at least 2 ng/ml. 19. The culture medium of claim 13, wherein said bFGF is provided at a concentration rage of 2-8 ng/ml. 20. A culture medium being devoid of serum and comprising bFGF, TGFβ1, transferrin and insulin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state. 21. The culture medium of claim 20, wherein the culture medium is free of an animal contaminant. 22. The culture medium of claim 20, wherein the culture medium is free of feeder cell contaminants. 23. The culture medium of claim 20, wherein said TGFβ1 is provided at a concentration range of 0.06-0.24 ng/ml. 24. The culture medium of claim 20, wherein said bFGF is provided at a concentration of at least 2 ng/ml. 25. The culture medium of claim 20, wherein said bFGF is provided at a concentration rage of 2-8 ng/ml.
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이 특허에 인용된 특허 (30)
Amit, Michal; Itskovitz-Eldor, Joseph, Co-culturing mammalian embryonic stem cells with human foreskin fibroblasts.
Gold, Joseph D.; Hassanipour, Mohammad; Collins, Lila R.; Xu, Chunhui, Direct differentiation method for making cardiomyocytes from human embryonic stem cells.
Gold,Joseph D.; Hassanipour,Mohammad; Collins,Lila R.; Xu,Chunhui, Direct differentiation method for making cardiomyocytes from human embryonic stem cells.
Mandalam, Ramkumar; Xu, Chunhui; Gold, Joseph D.; Carpenter, Melissa K., Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor.
Eriksson, Peter; Kilmare, Eva Karin; Tallheden, Tommi; Enerbäck, Sven, Method for efficient transfer of human blastocyst-derived stem cells (hBS cells) from a feeder-supported to a feeder-free culture system, long-term propagation of hBS cells under feeder-free conditions and use of cultured hBS cells for applications in myocardial regeneration.
Amit, Michal; Itskovitz-Eldor, Joseph, Methods of culturing cells in a medium comprising transforming growth factor beta 1 and basic fibroblast growth factor.
Wilson Elaine L. (New York NY) Gabrilove Janice (New York NY), Stimulation, production and culturing of hematopoietic progenitor cells by fibroblast growth factors.
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