초록 ▼

Embodiments provided herein concern tobacco and tobacco products having a reduced amount of a harmful compound. More specifically, several embodiments concern approaches to modify the expression of a gene that is involved in the production of a harmful compound in tobacco, tobacco products made usin

Embodiments provided herein concern tobacco and tobacco products having a reduced amount of a harmful compound. More specifically, several embodiments concern approaches to modify the expression of a gene that is involved in the production of a harmful compound in tobacco, tobacco products made using these approaches and methods of determining whether the removal of said compounds using said approaches yields a tobacco and/or a tobacco product that has a reduced potential to contribute to a tobacco-related disease.

대표청구항 ▼

1. A cured transgenic tobacco comprising a first heterologous nucleic acid molecule, wherein said nucleic acid molecule comprises: a) SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 33; orb) a complement of the nucleic acid sequence of SEQ ID NO: 26, SEQ ID NO: 27 or SEQ ID NO: 33. 2. The cured transgenic

1. A cured transgenic tobacco comprising a first heterologous nucleic acid molecule, wherein said nucleic acid molecule comprises: a) SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 33; orb) a complement of the nucleic acid sequence of SEQ ID NO: 26, SEQ ID NO: 27 or SEQ ID NO: 33. 2. The cured transgenic tobacco of claim 1, wherein said cured transgenic tobacco comprises an amount of nornicotine that is less than or equal to 0.2 mg/g of total biomass. 3. The cured transgenic tobacco of claim 1, wherein said cured transgenic tobacco comprises an amount of nicotine that is less than or equal to 4 mg/g of total biomass. 4. The cured transgenic tobacco of claim 1, wherein said cured transgenic tobacco comprises an amount of nicotine that is 2.17 to 3.99 mg/g of total biomass and an amount of nornicotine that is less than or equal to 0.00 to 0.06 mg/g of total biomass. 5. The cured transgenic tobacco of claim 1, wherein said cured transgenic tobacco comprises a collective content of TSNAs of less than or equal to 5.0 μg/g, 4.0 μg/g, 3.0μg/g, 2.0 μg/g, 1.0 μg/g, 0.5 μg/g, or 0.2 μg/g of total biomass. 6. The cured transgenic tobacco of claim 1, wherein said transgenic tobacco comprises an amount of nicotine that is less than or equal to 1.0 mg/g, 0.6 mg/g, 0.3 mg/g, or 0.05 mg/g of total biomass and a collective content of TSNAs of less than or equal to 5.0 μg/g, 4.0 μg/g, 3.0 μg/g, 2.0 μg/g, 1.0 μg/g, 0.5 μg/g, or 0.2 μg/g of total biomass. 7. A seed, reproductive tissues, vegetative tissues, biomass, leaves, plant parts or progeny derived from the transgenic tobacco of claim 1, wherein said seed, reproductive tissues, vegetative tissues, biomass, leaves, plant parts or progeny comprises said nucleic acid molecule. 8. A tobacco product derived from the transgenic tobacco of claim 1, wherein said tobacco product comprises said nucleic acid molecule. 9. The tobacco product of claim 8, further defined as a cigarette.