Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.
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1. A microfluidic workpiece, comprising: a microfluidic channel being impressed in the surface of a thermoplastic substrate,the microfluidic channel defining a depth and the microfluidic channel comprising a characteristic dimension in the range of from about 0.5 micrometers to about 100 micrometers
1. A microfluidic workpiece, comprising: a microfluidic channel being impressed in the surface of a thermoplastic substrate,the microfluidic channel defining a depth and the microfluidic channel comprising a characteristic dimension in the range of from about 0.5 micrometers to about 100 micrometers,the microfluidic channel being adjacent to at least one secondary region, the secondary region defining a depth that is less than the depth of the microfluidic channel;a wall defining a boundary between the microfluidic channel and the secondary region; andat least one probe residing on a surface of the microfluidic channel;a radiation-absorbing compound residing in the secondary region, wherein the wall separates the radiation-absorbing compound from the microfluidic channel; anda thermoplastic member that is disposed above and opposite to the thermoplastic substrate and that at least partially overlaps the microfluidic channel of the thermoplastic substrate, the thermoplastic member being bonded to the thermoplastic substrate at the location of the radiation-absorbing compound. 2. The microfluidic workpiece of claim 1, wherein the wall comprises a characteristic dimension in the range of from about 0.5 micrometers to about 100 micrometers. 3. The microfluidic workpiece of claim 1, wherein the microfluidic channel comprises at least one roughened surface. 4. The microfluidic workpiece of claim 1, wherein the wall resides proximate to the microfluidic channel. 5. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate is essentially transparent to light. 6. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate is essentially transparent to at least a portion of the visible light spectrum. 7. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate is essentially transparent to at least a portion of the infrared light spectrum. 8. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate is essentially transparent to at least a portion of the ultraviolet light spectrum. 9. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate is essentially transparent to light having a wavelength in the range of from 1 nm to about 1 mm. 10. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate comprises a polyolefin, a cyclic olefin, a polyacrylic, a polystyrene, a polycarbonate, a polyimide, a polyacrylonitrile, a polyester, a polyarylamide, a polyamide, a polyetherketone, a polyvinyl halide, or any copolymer or combination thereof. 11. The microfluidic workpiece of claim 10, wherein the polyolefin comprises a polypropylene, a polyethylene, a cyclic polyolefin, a cyclic olefin, or any copolymer or combination thereof. 12. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate comprises at least one cyclic molecule. 13. The microfluidic workpiece of claim 1, wherein the thermoplastic substrate comprises at least one bridged cyclic molecule. 14. The microfluidic workpiece of claim 1, further comprising at least one probe residing within a porous medium situated within the microfluidic channel. 15. The microfluidic workpiece of claim 14, wherein the porous medium is a polymer. 16. The microfluidic workpiece of claim 1, further comprising a filter in fluidic communication with the microfluidic channel, wherein the filter is capable of filtering molecules from a liquid sample. 17. The microfluidic workpiece of claim 16, wherein the filter is characterized as having a nominal pore size of less than about 2 microns. 18. The microfluidic workpiece of claim 16, wherein the filter is characterized as having a nominal pore size in the range of from about 0.02 microns to about 2 microns. 19. The microfluidic workpiece of claim 16, wherein the filter is characterized as having a nominal pore size in the range of from 0.2 microns to about 0.5 microns. 20. The microfluidic workpiece of claim 1, the thermoplastic member having, as compared to the thermoplastic substrate, one or more of: a higher Tg, a higher deflection temperature under load, or a lower melt volume rate. 21. The microfluidic workpiece of claim 1, wherein the microfluidic channel comprises a characteristic dimension in the range of from about 0.5 micrometers to less than 5 micrometers. 22. A microfluidic workpiece, comprising: a microfluidic channel being impressed in the surface of a thermoplastic substrate,the microfluidic channel defining a depth and the microfluidic channel comprising a characteristic dimension in the range of from about 0.5 micrometers to about 100 micrometers,the microfluidic channel being adjacent to at least one secondary region,a wall defining a boundary between the microfluidic channel and the secondary region;at least one probe residing on a surface of the microfluidic channel; anda radiation-absorbing compound residing in the secondary region, wherein the wall separates the radiation-absorbing compound from the microfluidic channel; anda thermoplastic member that is disposed above and opposite to the thermoplastic substrate and that at least partially overlaps the microfluidic channel of the thermoplastic substrate, the thermoplastic member being bonded to the thermoplastic substrate at the location of the radiation-absorbing compound. 23. The microfluidic workpiece of claim 22, wherein the secondary region comprises a trench.
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