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Manipulation of fluids and reactions in microfluidic systems

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • G01N-001/38
  • B01L-003/00
  • C30B-007/00
  • C30B-029/58
  • B01L-003/06
  • B01L-007/00
출원번호 US-0577652 (2014-12-19)
등록번호 US-9440232 (2016-09-13)
발명자 / 주소
  • Fraden, Seth
  • Cristobal-Azkarate, Galder
출원인 / 주소
  • Raindance Technologies, Inc.
대리인 / 주소
    Brown Rudnick LLP
인용정보 피인용 횟수 : 0  인용 특허 : 460

초록

Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a mic

대표청구항

1. A method, comprising: providing a microfluidic network comprising five or more sections in a serial arrangement, each section comprising a first microchannel, a second microchannel, a microwell, and a junction, wherein the junction is fluidicly coupled to the first and second microchannels and th

이 특허에 인용된 특허 (460)

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  193. Miller, Benjamin; Hutchison, Brian; Wilson, Andrew; Larson, Jonathan; Zhong, Qun; Yurkovetsky, Yevgeny; Link, Darren; Weary, Mark, Manipulation of microfluidic droplets.
  194. Brenner Sydney (Cambridge GB2), Massively parallel sequencing of sorted polynucleotides.
  195. Kricka Larry J. ; Wilding Peter, Mesoscale devices and methods for analysis of motile cells.
  196. Wilding Peter (Paoli PA) Kricka Larry J. (Berwyn PA), Mesoscale polynucleotide amplification device and method.
  197. Wilding Peter (Paoli PA) Kricka Larry J. (Berwyn PA), Mesoscale polynucleotide amplification devices.
  198. Wilding Peter ; Kricka Larry J., Mesoscale sample preparation device and systems for determination and processing of analytes.
  199. Kricka Larry J. (Berwyn PA) Wilding Peter (Paoli PA), Mesoscale sperm handling devices.
  200. Kricka Larry J. (Berwyn PA) Wilding Peter (Paoli PA), Mesoscale sperm handling devices.
  201. Hanninen Pekka,FIX ; Soini Erkki,FIX, Method and a device for monitoring nucleic acid amplification reactions.
  202. Quake Stephen ; Volkmuth Wayne D., Method and apparatus for analysis and sorting of polynucleotides based on size.
  203. Smythe William J. (Canterbury CT) Isreeli Jack (Mamaroneck NY) Pelavin Milton H. (Chappaqua NY), Method and apparatus for automated analysis of fluid samples.
  204. Cheng Alan T. (Bloomfield NJ), Method and apparatus for dispersing a gas into a liquid.
  205. Barbieri Girolamo (Via Montello ; 10 - 22069 Rovellasca (Como) ITX), Method and apparatus for electrostatically coating a workpiece with paint.
  206. Valaskovic, Gary A.; Lee, Mike S., Method and apparatus for feedback controlled electrospray.
  207. Stone, Howard A.; Anna, Shelley L.; Bontoux, Nathalie; Link, Darren R.; Weitz, David A.; Gitlin, Irina; Kumacheva, Eugenia; Garstecki, Piotr; Diluzio, Willow; Whitesides, George M., Method and apparatus for fluid dispersion.
  208. Stone, Howard A.; Anna, Shelley L.; Bontoux, Nathalie; Link, Darren Roy; Weitz, David A.; Gitlin, Irina; Kumacheva, Eugenia; Garstecki, Piotr; Diluzio, Willow R.; Whitesides, George M., Method and apparatus for fluid dispersion.
  209. Gamarnik Moisey Y. ; Alvarado Ulises R., Method and apparatus for growing crystals.
  210. Turock David L., Method and apparatus for implementing a computer network/internet telephone system.
  211. Suh Nam P. (Sudbury MA) Tucker ; III Charles L. (Medford both of MA), Method and apparatus for mixing particles.
  212. Pelavin Milton H. (Chappaqua NY), Method and apparatus for the non-invasive determination of the characteristics of a segmented fluid stream.
  213. Harris Michael T. (Knoxville TN) Scott Timothy C. (Knoxville TN) Byers Charles H. (Oak Ridge TN), Method and apparatus for the production of metal oxide powder.
  214. Harris Michael T. (Knoxville TN) Scott Timothy C. (Knoxville TN) Byers Charles H. (Oak Ridge TN), Method and apparatus for the production of metal oxide powder.
  215. Stock Heinz (Oberursel DT) Rebentisch ; deceased Hans W. (late of Frankfurt DT BY Waltraud Rebentisch ; heir at law), Method and device for metering and dispersing fluid materials.
  216. Alfonso Ganan-Calvo ES, Method and device for production of components for microfabrication.
  217. Ganan-Calvo Alfonso,ESX, Method and device for production of components for microfabrication.
  218. Franzen Jochen,DEX, Method and devices for extremely fast DNA replication by polymerase chain reactions (PCR).
  219. L?we, Holger; Schiewe, J?rg; Hessel, Volker; Dietrich, Thomas; Freitag, Andreas, Method and statistical micromixer for mixing at least two liquids.
  220. Chetverin Alexander B. (Moskovskaya oblast Puschino ; mikroraion AB ; 24 ; kv.238 RUX) Chetverina Helena V. (Moskovskaya oblast Puschino ; mikroraion AB ; 24 ; kv.238 RUX), Method for amplification of nucleic acids in solid media.
  221. Parris Norman Alfred, Method for analysis.
  222. Jido, Morio, Method for blending by combining fine particles.
  223. Stabinsky Yitzhak (Boulder CO), Method for derivitization of polynucleotides.
  224. Brown, James F.; Silver, Jonathan E., Method for detecting the presence of a single target nucleic acid in a sample.
  225. Brown, James F.; Silver, Jonathan E., Method for detecting the presence of a single target nucleic acid in a sample.
  226. Arnold Andrew, Method for diagnosing neoplasia by detecting expression of PRAD1 cyclin.
  227. Carson, Dennis A.; Schmid, Mathias; Carrera, Carlos J., Method for early diagnosis of, and determination of prognosis in, cancer.
  228. DePaoli David W. ; Tsouris Constantinos ; Feng James Q., Method for electrically producing dispersions of a nonconductive fluid in a conductive medium.
  229. Clark Steven C. (Winchester MA) Kaufman Randal J. (Boston MA) Wong Gordon G. (Cambridge MA), Method for identification and isolation of DNA encoding a desired protein.
  230. John Wallace Parce ; Anne R. Kopf-Sill, Method for in situ concentration and/or dilution of materials in microfluidic systems.
  231. Cuppens, Harry, Method for introducing a sample specific DNA tag into a plurality of DNA fragments from a plurality of samples.
  232. Radomski Christopher C. A.,CAX ; Seow Kah Tong,SGX ; Warren R. Antony J.,CAX ; Yap Wai Ho,SGX, Method for isolating xylanase gene sequences from soil DNA, compositions useful in such method and compositions obtained.
  233. Gale Stott, Method for making a faux stone concrete panel.
  234. Ismagilov, Rustem F.; Zheng, Bo; Gerdts, Cory J., Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems.
  235. Sato Naofumi (Saitama JPX) Shirakawa Kamon (Saitama JPX), Method for measuring adenyl group-containing substances using heteropoly-acid.
  236. Adams Christopher P. (Winter Hill MA) Kron Stephen Joseph (Boston MA), Method for performing amplification of nucleic acid with two primers bound to a single solid support.
  237. Ismagliov, Rustem F.; Tice, Joshua David; Gerdts, Cory John; Zheng, Bo, Method for performing crystallization and reactions in pressure-driven fluid plugs.
  238. Kasai Shigetami,JPX ; Okada Hisatake,JPX, Method for preparing silver halide emulsion.
  239. Bibette, Jerome; Ficheux, Marie-Fran.cedilla.oise; Leal Calderon, Fernando; Bonnakdar, Lida, Method for releasing an active principle contained a multiple emulsion.
  240. Lau, Derick H.; Lam, Kit S., Method for screening combinatorial bead library, capturing cells from body fluids, and ligands for cancer cells.
  241. Santarsiero, Bernard D.; Stevens, Raymond C.; Schultz, Peter G.; Jaklevic, Joseph M.; Yegian, Derek T.; Cornell, Earl W.; Nordmeyer, Robert A., Method for screening microcrystallizations for crystal formation.
  242. Jido Morio (Tokyo JPX), Method for spray combination of liquids and apparatus therefor.
  243. Lapidus Stanley N. (Bedford NH) Shuber Anthony P. (Milford MA) Ulmer Kevin M. (Cohasset MA), Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample.
  244. Michael Wolberg DE; Michael Muller DE; Werner Hummel DE, Method for the enantioselective reduction of 3,5-dioxocarboxylic acids, their salts and their esters.
  245. Sheridon Nicholas K. (Los Altos CA), Method for the fabrication of multicolored balls for a twisting ball display.
  246. Benecke Wolfgang (Berlin DEX) Wagner Bernd (Berlin DEX) Hagedorn Rolf (Berlin DEX) Fuhr Gnter (Berlin DEX) Mller Torsten (Berlin DEX), Method of continuously separating mixtures of microscopic dielectric particles and apparatus for carrying through this m.
  247. Sidransky, David, Method of detection of prostate cancer.
  248. Ali,Shujath M.; Sun,Yongming; Salceda,Susana; Recipon,Herve; Cafferkey,Robert, Method of diagnosing, monitoring, staging, imaging and treating prostate cancer.
  249. Ali,Shujath; Cafferkey,Robert; Recipon,Herve; Sun,Yongming, Method of diagnosing, monitoring, staging, imaging and treating prostate cancer.
  250. Salceda,Susana; Recipon,Herve; Cafferkey,Robert, Method of diagnosing, monitoring, staging, imaging and treating prostate cancer.
  251. Davi,Frederic; Dighiero,Guillaume; Dumas,Gerard; Oppezzo,Pablo; Settegrana,Catherine; Vasconcelos Pinheiro,Yuri, Method of diagnosis/prognosis of human chronic lymphocytic leukemia comprising the profiling ofgenes.
  252. Enoch Kim ; Younan Xia ; Milan Mrksich ; Rebecca J. Jackman ; Xiao-Mei Zhao ; Stephen P. Smith ; Mara G. Prentiss ; George M. Whitesides ; Christian Marzolin FR, Method of forming articles including waveguides via capillary micromolding and microtransfer molding.
  253. Ando,Kiyoshi; Hotta,Tomomitsu; Ito,Chie; Sato,Hidenao; Furuya,Akiko; Shitara,Kenya; Sugimoto,Seiji; Kohno,Hiroaki, Method of judging leukemia, pre-leukemia or aleukemic malignant blood disease and diagnostic therefor.
  254. Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel; Quake, Stephen R., Method of making a microfabricated elastomeric valve.
  255. Brown James F. ; Silver Jonathan E. ; Kalinina Olga V.,CAX, Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly.
  256. James F. Brown ; Jonathan E. Silver ; Olga V. Kalinina CA, Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly.
  257. Nyren P.ang.l,SEX ; Uhlen Mathias,SEX ; Ronaghi Mostafa,SEX, Method of sequencing DNA.
  258. Nyren Pal,SEX, Method of sequencing DNA based on the detection of the release of pyrophosphate and enzymatic nucleotide degradation.
  259. Rothberg Jonathan M. ; Bader Joel S., Method of sequencing a nucleic acid.
  260. Brenner Sydney,GB2, Method of sorting polynucleotides.
  261. Griffiths, Andrew David; Abell, Chris; Hollfelder, Florian; Mastrobattista, Enrico, Method of synthesis and testing of combinatorial libraries using microcapsules.
  262. Scott Timothy C. (Knoxville TN), Method of using an electric field controlled emulsion phase contactor.
  263. Huttemann,Maik, Method to detect lung cancer.
  264. Brenner Sydney,GBX, Method, apparatus and computer program product for determining a set of non-hybridizing oligonucleotides.
  265. Quake, Stephen; Unger, Marc, Methods and apparatus for analyzing polynucleotide sequences.
  266. Kelly Arnold J. (Princeton Junction NJ), Methods and apparatus for dispersing a fluent material utilizing an electron beam.
  267. Santarsiero Bernard D. ; Stevens Raymond C. ; Shultz Peter G. ; Jaklevic Joseph M. ; Yegian Derek T. ; Cornell Earl ; Nordmeyer Robert A. ; Jin Jian ; Kolbe William F. ; Jones Arthur L. ; Uber Donald, Methods and apparatus for performing array microcrystallizations.
  268. Pardee,Arthur B.; Ford,Heide L., Methods and compositions for diagnosing and predicting the behavior of cancer.
  269. Brenner,Sydney, Methods and compositions for tagging and identifying polynucleotides.
  270. Genetta,Thomas, Methods and compositions useful for diagnosis, staging, and treatment of cancers and tumors.
  271. Harris, Timothy; Buzby, Philip Richard; Jarosz, Mirna; DiMeo, James Joseph; Gill, Jaime, Methods and devices for nucleic acid sequence determination.
  272. Kaduchak, Gregory; Stewart, Carleton; Ward, Michael, Methods and systems for controlling the flow of particles for detection.
  273. Stephen Quake ; Wayne D. Volkmuth, Methods for analysis and sorting of polynucleotides.
  274. Quake, Stephen R.; Chou, Hou-Pu, Methods for detecting and sorting polynucleotides based on size.
  275. Polsky,David; Osman,Iman; Chapman,Paul B., Methods for detecting circulating mutant BRAF DNA.
  276. Lapidus Stanley N. ; Shuber Anthony P., Methods for detecting differences in RNA expression levels and uses therefor.
  277. Muramatsu,Takashi; Okamoto,Kohji; Ikematsu,Shinya; Oda,Munehiro; Kumai,Hideshi; Sakuma,Sadatoshi, Methods for detecting early cancer.
  278. Skolnick Mark H. ; Cannon-Albright Lisa A. ; Kamb Alexander, Methods for detecting predisposition to cancer at the MTS gene.
  279. Roberts James M. ; Porter Peggy L. ; Polyak Kornelia ; Massague Joan ; Koff Andrew, Methods for determining isolated p27 protein levels and uses thereof.
  280. Reed,John C., Methods for determining the prognosis for cancer patients using tucan.
  281. Taniguchi Tadatsugu (Ibaraki JPX) Harada Hisashi (Suita JPX), Methods for diagnosing cancer, precancerous state, or susceptibility to other forms of diseases by detecting an accelera.
  282. Peterson Todd C. ; Foster Lyndon M. ; Brian Paul, Methods for generating and screening novel metabolic pathways.
  283. Gold Larry (Boulder CO) Tuerk Craig (Boulder CO), Methods for identifying nucleic acid ligands.
  284. Harris,Timothy D., Methods for increasing accuracy of nucleic acid sequencing.
  285. Perlman Daniel (Arlington MA), Methods for isolating mutant microoganisms using microcapsules coated with indicator material.
  286. Wang,Xiaobo; Wu,Lei; Cheng,Jing; Yang,Weiping; Xu,Junquan, Methods for manipulating moieties in microfluidic systems.
  287. Brenner Sydney (Cambridge GB2), Methods for sorting polynucleotides using oligonucleotide tags.
  288. Shuber Anthony P. ; Lapidus Stanley N., Methods for the detection of chromosomal aberrations.
  289. Lapidus Stanley N. ; Shuber Anthony P., Methods for the detection of loss of heterozygosity.
  290. Shuber Anthony P. ; Lapidus Stanley N. ; Daley George Q., Methods for the detection of nucleic acids.
  291. Giordano Antonio, Methods for the diagnosis and prognosis of cancer.
  292. O'Brien Timothy J., Methods for the early diagnosis of ovarian cancer.
  293. O'Brien Timothy L., Methods for the early diagnosis of ovarian cancer.
  294. O'Brien Timothy J., Methods for the early diagnosis of ovarian, breast and lung cancer.
  295. Dai,Hongyue; He,Yudong; Linsley,Peter S.; Mao,Mao; Roberts,Christopher J.; Van't Veer,Laura Johanna; Van de Vijver,Marc J.; Bernards,Rene; Hart,A. A. M., Methods of assigning treatment to breast cancer patients.
  296. Brown, James F.; Silver, Jonathan E., Methods of detecting amplified product.
  297. Mack, David; Gish, Kurt C.; Wilson, Keith E., Methods of diagnosing and determining prognosis of colorectal cancer.
  298. Feinstein,Elena; Mor,Orna, Methods of diagnosing bladder cancer.
  299. Ogden, Christopher William; Adshead, James; Kessling, Anna Maria; Khoubehi, Bijan, Methods of diagnosing prostate cancer through the detection of the presence or absence of Pax 2 mRNA.
  300. Waldman, Scott A.; Park, Jason; Schulz, Stephanie, Methods of screening and diagnosing esophageal cancer by determining guanylin cyclase C expression.
  301. Gert Blankenstein DE, Micro-flow system for particle separation and analysis.
  302. Kamahori Masao (Saitama JPX), Micro-reactor device for minute sample analysis.
  303. He,Lin; Peng,Jinlin; Shi,Youchun; Webb,Brian L.; Yuen,Po Ki, Microcolumn-based, high-throughput microfluidic device.
  304. Mank Reinhard (Kiel-Russee DEX) Gustafsson Jan (Kiel DEX) Hrig Joachim (Kiel-Kronshagen DEX) Kchling Wolfgang (Tttendorf DEX) Nerlich Birgit (Kiel-Russee DEX), Microencapsulation of water-soluble medicaments.
  305. Spence, Charles F.; Fu, Anne Y.; Quake, Stephen R.; Arnold, Frances H., Microfabricated cell sorter for chemical and biological materials.
  306. Quake,Stephen R.; Thorsen,Todd, Microfabricated crossflow devices and methods.
  307. Jacobson Stephen C. ; Ramsey J. Michael, Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same.
  308. Yager Paul ; Brody James P. ; Holl Mark R. ; Forster Fred K. ; Galambos Paul C., Microfabricated differential extraction device and method.
  309. Marc A. Unger ; Hou-Pu Chou ; Todd A. Thorsen ; Axel Scherer ; Stephen R. Quake, Microfabricated elastomeric valve and pump systems.
  310. Andersson, Per; Derand, Helene; Gustafsson, Magnus; Palm, Anders; Wallenborg, Sussanne, Microfludic system (EDI).
  311. Staats, Sau Lan Tang, Microfluidic array devices and methods of manufacture and uses thereof.
  312. Huang, Jiang; Chou, Hou-Pu; Unger, Marc A., Microfluidic chromatography.
  313. Jacobson, Stephen C.; Ramsey, J. Michael, Microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream.
  314. Unger,Marc; Manger,Ian D.; Lucero,Michael; Yi,Yong; Miyashita Lin,Emily; Weinecke,Anja; Facer,Geoffrey, Microfluidic device and methods of using same.
  315. Ramsey, J. Michael; Jacobson, Stephen C., Microfluidic devices for the controlled manipulation of small volumes.
  316. Chow, Andrea W.; Kopf-Sill, Anne R.; Parce, J. Wallace; Sundberg, Steven A., Microfluidic devices, systems and methods for performing integrated reactions and separations.
  317. Fraden, Seth; Link, Darren Roy; Cristobal Azkarate, Galder; Shim, Jung uk; Weitz, David A., Microfluidic manipulation of fluids and reactions.
  318. David, Peter R., Microfluidic method employing delivery of plural different fluids to same lumen.
  319. Parce J. Wallace ; Knapp Michael R., Microfluidic sampling system and methods.
  320. Wang,Mark; Ata,Erhan Polatkan; Esener,Sadik C., Microfluidic sorting device.
  321. Ekstrm Bjrn (Upsala SEX) Jacobson Gunilla (Upsala SEX) Ohman Ove (Upsala SEX) Sjdin Hakan (Upsala SEX), Microfluidic structure and process for its manufacture.
  322. Desai Amish ; Yang Xing ; Tai Yu-Chong ; Marzluff Elaine ; Bockenkamp Dirk ; Mayo Stephen, Microfluidic sub-millisecond mixers.
  323. Harrison, Daniel Jed; Andersson, Per; Li, Paul C. H.; Szarka, Roderick; Smith, Richard, Microfluidic system and methods of use.
  324. Anderson, Janelle R.; Cherniavskaya, Oksana; Chiu, Daniel T.; Jackman, Rebecca J.; McDonald, Cooper; Whitesides, George M., Microfluidic systems including three-dimensionally arrayed channel networks.
  325. Quake, Stephen R.; Brody, James P., Microlensing particles and applications.
  326. Nishimaki Fukumi,JPX ; Takahashi Nobuhiro,JPX ; Tsuchida Tomohiko,JPX ; Watanabe Kazuya,JPX ; Hino Sanae,JPX, Microorganisms, demulsifiers and processes for breaking an emulsion.
  327. Chandler Mark B. ; Chandler Don J., Microparticles attached to nanoparticles labeled with flourescent dye.
  328. Pantoliano, Michael W.; Petrella, Eugenio C.; Salemme, F. Raymond; Springer, Barry A., Microplate thermal shift assay for ligand development using 5-(4"dimethylaminophenyl)-2-(4'-phenyl)oxazole derivative fluorescent dyes.
  329. Karger Barry L. ; Foret Frantisek ; Zavracky Paul M. ; McGruer E. Nicol ; Xue Qifeng ; Dunayevskiy Yuriy M., Microscale fluid handling system.
  330. Hilfinger John M. ; Davidson Beverly L. ; Beer Steven J. ; Crison John R. ; Amidon Gordon L., Microsphere encapsulation of gene transfer vectors.
  331. Levy, Robert J.; Labhasetwar, Vinod; Cohen, Hagit, Microspheres containing condensed polyanionic bioactive agents and methods for their production.
  332. Tanimori Toru,JPX ; Ochi Atsuhiko,JPX, Microstrip gas chamber high-speed data acquisition system and method of measuring samples by use of the system.
  333. David, Peter R.; David, Nathaniel E., Microvolume crystallization method employing multiple lumens.
  334. Gravesen Peter (Nordborg DKX) Branebjerg Jens A. (N rum DKX), Miniature actuating device.
  335. Aine Harry E. (30600 Page Mill Rd. Los Altos CA 94022) Block Barry (30610 Page Mill Rd. Los Altos CA 94022), Miniature valve and method of making same.
  336. Kim, Enoch; Xia, Younan; Mrksich, Milan; Jackman, Rebecca J.; Zhao, Xiao-Mei; Smith, Stephen P.; Marzolin, Christian; Prentiss, Mara G.; Whitesides, George M., Molded waveguides.
  337. Nacht,Mariana; Dracheva,Tatiana; Sidransky,David; Madden,Stephen; Jen,Jin, Molecular characteristics of non-small cell lung cancer.
  338. Golovchenko,Jene A.; Branton,Daniel, Molecular characterization with carbon nanotube control.
  339. Brenner, Sydney; Macevicz, Stephen C., Molecular counting.
  340. Chan Eugene Y., Molecular motors.
  341. Handique Kalyan ; Gogoi Bishnu ; Burns Mark A. ; Mastrangelo Carlos H., Moving microdroplets.
  342. Lei, Ming; Adey, Nils B.; McNeely, Michael R., Multi-channel microfluidic system design with balanced fluid flow distribution.
  343. Chatteriee Dilip K. (Rochester NY) Paz-Puzalt Gustavo R. (Rochester NY), Multi-zone heating for a fuser roller.
  344. Krafft Marie-Pierre,FRX ; Riess Jean G.,FRX ; Zarif Leila, Multiple emulsions comprising a hydrophobic continuous phase.
  345. Church George M. (Boston MA) Kieffer-Higgins Stephen (Dorchester MA), Multiplex analysis of DNA.
  346. Kennedy Colin B., Multiplexed microfluidic devices and systems.
  347. Robbins,David; Lin Goerke,Juili Lillian; Ling,Jessica C., Mutations in human MLH1 and human MSH2 genes useful in diagnosing colorectal cancer.
  348. Kobayashi William T. (Sao Paulo BRX) Francis ; Jr. Arthur W. (Monroe NY), Narrow spray angle liquid fuel atomizers for combustion.
  349. Guenkel Alfred A. (Vancouver CAX) Rae John M. (Vancouver CAX) Hauptmann Edward G. (West Vancouver CAX), Nitration process.
  350. Haak-Frendscho Mary ; Okragly Angela J. ; Niles Andrew L. ; Saban Ricardo, Non-invasive diagnostic method for interstitial cystitis and bladder cancer.
  351. Quake, Stephen; Fan, Hei-Mun Christina, Non-invasive fetal genetic screening by digital analysis.
  352. Sato Kozo (No. 7-12 ; Minami-3-chome ; Yamamoto-cho Yao ; Osaka JA), Nozzle of gas cutting torch.
  353. Enzelberger, Markus M.; Liu, Jian; Quake, Stephen R., Nucleic acid amplification utilizing microfluidic devices.
  354. Brenner, Sydney, Nucleic acid analysis using sequence tokens.
  355. Guilfoyle Richard A. ; Guo Zhen, Nucleic acid indexing.
  356. Gold Larry (Boulder CO) Tuerk Craig (Boulder CO), Nucleic acid ligands.
  357. Kacian Daniel L. (San Diego CA) Fultz Timothy J. (Vista CA), Nucleic acid sequence amplification methods.
  358. Friedman Jeffrey M. ; Zhang Yiying ; Proenca Ricardo, OB polypeptide antibodies and method of making.
  359. Brenner Sydney,GB2 ; Albrecht Glenn ; Macevicz Stephen C., Oligonucleotide tags for sorting and identification.
  360. Brenner Sydney,GBX, Oligonucleotide tags for sorting and identification.
  361. Brenner Sydney,GBX, Oligonucleotide tags for sorting and identification.
  362. Brenner Sydney,GBX ; Albrecht Glenn ; Macevicz Stephen C., Oligonucleotide tags for sorting and identification.
  363. Sydney Brenner GB, Oligonucleotide tags for sorting and identification.
  364. Van Kazuo (Nara JPX) Ohta Kenji (Nara JPX) Inui Tetsuya (Nara JPX) Nagaura Toshikazu (Nara JPX) Takemori Hirotoshi (Nara JPX) Toki Hirotaka (Kashihara JPX), Optical disk for use in optical memory devices.
  365. Griffiths, Andrew; Tawfik, Dan; Sepp, Armin, Optical sorting method.
  366. Griffiths, Andrew; Tawfik, Dan; Sepp, Armin, Optical sorting method.
  367. Apffel James A. ; Werlich Mark H. ; Bertsch James L. ; Goodley Paul C., Orthogonal ion sampling for APCI mass spectrometry.
  368. Platica,Micsunica; Platica,Ovidu; Holland,James F., PAR, a novel marker gene for breast and prostate cancers.
  369. McCaffey,Ian; Alving,Kim; Domon,Bruno; Ruben,Steven, Pancreatic cancer secreted targets and uses thereof.
  370. Domon,Bruno; McCaffery,Ian; Narayan,Vaibhav; Patterson,Scott, Pancreatic cancer targets and uses thereof.
  371. LaRocca William J. (Sterling Heights MI) Trush David M. (Warren MI), Parking brake assembly.
  372. Anderson,Janelle R.; Chiu,Daniel T.; Jeon,Noo Li; Huang,Sui; Kane,Ravindra; Choi,Insung S.; Ingber,Donald E.; Whitesides,George M., Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks.
  373. Hart Charles P., Peptide library and screening method.
  374. Malcolm ; Jr. Ronald D. ; Lutz Juergen, Phase comparison and phase adjustment for synchronization to a reference signal that is asynchronous with respect to a digital sampling clock.
  375. Singh Akhileshwar R., Plumbing fixtures and fittings employing copper-bismuth casting alloys.
  376. Tan Hsiaoming Sherman, Pneumatic concentric nebulizer with adjustable and capillaries.
  377. Hare Robert V. (Georgetown DE), Polyvinylsiloxane impression material.
  378. Erlander,Mark G.; Ma,Xiao Jun; Sgroi,Dennis C., Predicting outcome with tamoxifen in breast cancer.
  379. Baker,Joffre B.; Bryant,John L.; Paik,Soonmyung; Shak,Steven, Prediction of likelihood of cancer recurrence.
  380. David Claire (Paris FRX), Preparation of ammonium rare earth double oxalates and rare earth oxides produced therefrom.
  381. Sutton Andrew D. (Ruddington GB2) Johnson Richard A. (West Bridgford GB2) Senior Peter J. (Near Melbourne GB2) Heath David (The Park GB2), Preparation of diagnostic agents.
  382. Bcheler Manfred (Overath DEX) Gehringer Hans (Cologne DEX) Klinksiek Bernd (Bergisch Gladbach DEX) Koglin Bernd (Bergisch Gladbach DEX), Preparation of pharmaceutical or cosmetic dispersions.
  383. McGibbon Graeme (Whitley Bay GB2), Preparation of titanium derivatives.
  384. Kosak Kenneth M. (3194 S. 4400 W. West Valley City UT 84120) Kosak Matthew K. (3194 S. 4400 W. West Valley City UT 84120), Preparation of wax beads containing a reagent for release by heating.
  385. Coffee Ronald A. (Thursley Copse ; Farnham Lane Haslemere ; Surrey GBX), Process and apparatus for spraying liquid.
  386. Reneker, Darrell H., Process and apparatus for the production of nanofibers.
  387. Sizer Charles E. ; Palaniappan Sevugan, Process and article for determining the residence time of a food particle.
  388. Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
  389. Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
  390. Erlich Henry A. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA) Mullis Kary B. (Kensington CA), Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids.
  391. Benecke Wolfgang,DEX ; Wagner Bernd,DEX ; Fuhr Gunter,DEX ; Hagedorn Rolf,DEX ; Muller Thorsten,DEX, Process for manipulating microscopic, dielectric particles and a device therefor.
  392. Higuchi,Toshiro; Torii,Toru; Nishisako,Takashi; Taniguchi,Tomohiro, Process for producing emulsion and microcapsules and apparatus therefor.
  393. Serafin Mark, Process for production of heat sensitive dispersions or emulsions.
  394. Dollat Jean-Marie (Montlucon FRX) Molin Marc (Neris Les Bains FRX) Theallier Pascal (Montlucon FRX), Process for the preparation of spherules of active principles and emulsions containing the spherules.
  395. Unger Evan C., Prodrugs comprising fluorinated amphiphiles.
  396. Harris, Jennifer L.; Backes, Bradley J.; Ellman, Jonathan A.; Craik, Charles S., Profiling of protease specificity using combinatorial fluorogenic substrate libraries.
  397. Golub, Todd R.; Febbo, Phillip G.; Ross, Kenneth N.; Sellers, William R., Prostate cancer diagnosis and outcome prediction by expression analysis.
  398. Bernhard H. Weigl ; Jurgen Sygusch CA, Protein crystallization in microfluidic structures.
  399. Gray Joe W. ; Weier Heinz-Ulrich G., Quantitative DNA fiber mapping.
  400. Bae,Suk Chul; Ito,Yoshiaki, RUNX3 gene showing anti-tumor activity and use thereof.
  401. Kedar Haim, Reaction vessel agitation apparatus.
  402. Malkin, Daniel D.; Simbirtsev, Alexei V.; Peredkov, Boris A.; Kruglov, Gennady A., Reduction gear of electronic wristwatch with stepping motor and sweep second hand.
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