Cell culture apparatus, cell culture system and cell culture method
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12M-001/34
C12M-001/00
C12M-001/06
C12M-001/26
출원번호
US-0450481
(2008-04-25)
등록번호
US-9441193
(2016-09-13)
우선권정보
JP-2007-119112 (2007-04-27)
국제출원번호
PCT/JP2008/058008
(2008-04-25)
§371/§102 date
20090928
(20090928)
국제공개번호
WO2008/136371
(2008-11-13)
발명자
/ 주소
Tanaka, Satoshi
Ishizaki, Yoichi
Suenaga, Ryo
Tokita, Yoriko
Kogure, Masahito
출원인 / 주소
TOYO SEIKAN GROUP HOLDINGS, LTD.
대리인 / 주소
Osha Liang LLP
인용정보
피인용 횟수 :
0인용 특허 :
10
초록▼
By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attain
By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained. A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and/or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.
대표청구항▼
1. A cell culture method comprising: placing on an upper surface of a container table a culture container formed of a soft packing material;dividing an enclosure part of the culture container into two or more chambers by placing on an upper surface of the culture container a movable partition roller
1. A cell culture method comprising: placing on an upper surface of a container table a culture container formed of a soft packing material;dividing an enclosure part of the culture container into two or more chambers by placing on an upper surface of the culture container a movable partition roller so as to form a culture part for enclosing a culture medium and/or cells being cultured;pressing the upper surface of the culture container with the partition roller such that a first inner surface of the culture container contacts a second inner surface of the culture container opposite the first inner surface so as to segregate the two or more chambers from each other; andchanging a volume of the culture part by moving a rotation axis of the partition roller with the partition roller in a radial direction relative to the culture container, while allowing the partition roller to rotate and maintaining the partition roller to press the upper surface of the culture container such that the first inner surface of the culture container contacts the second inner surface of the culture container,wherein the two or more chambers comprise an extensible part, and the partition roller segregates the extensible part from the culture part so as to enclose an interior space of the culture part from the extensible part, andwherein a volume of an interior space of the culture part changes in a continuous manner while moving the partition roller and/or the culture container. 2. The cell culture method according to claim 1, further comprising: moving the partition roller towards a tube for harvest that is attached to the culture container; andpushing the culture medium and/or the cells being culture enclosed in the enclosure part to the tube for harvest so as to send the culture medium and/or the cells being cultured to a harvest container connected to the tube for harvest. 3. The cell culture method according to claim a 1, wherein the two or more chambers are not intercommunicative by the culture medium and/or the cells from each other. 4. The cell culture method according to claim 1, wherein the culture container comprises an opening, and at least a tapered form in which a cross-section of an interior space of the culture container becomes gradually narrower towards the opening. 5. The cell culture method according to claim 1, further comprising: measuring a condition of the culture medium and/or cells being cultured; andmoving the partition roller and/or the culture container based on measurement results obtained by the measuring. 6. The cell culture method according to claim 1, wherein the dividing is performed by holding the culture container between the partition roller and a trough-like member, andwherein the trough-like member has an arc-shaped cross section and is provided outside an outer peripheral surface of the partition roller. 7. The cell culture method according to claim 6, further comprising: pushing the enclosure part using an outer wall of the trough-like member when the partition roller moves. 8. The cell culture apparatus according to claim 7, wherein the trough-like member has a cylindrical shape, and an arc of the trough-like member and an outer peripheral circumference of the roller are concentrically formed. 9. The cell culture method according to claim 1, wherein an outer periphery of a cross section in a radial direction of the partition roller has a teeth-like structure, and each mountain part and each valley part of the teeth-like structure are in a straight line or in a curved line in an axial direction of the partition roller. 10. The cell culture method according to claim 1, wherein the dividing is performed by holding the culture container between two partition rollers. 11. The cell culture method according to claim 1, wherein the pressing is performed by applying a pressure of 0.1 MPa or more when the partition roller is placed on the upper surface of the culture container. 12. The cell culture method according to claim 1, wherein an outer peripheral surface of the partition roller is formed of at least one selected from the group consisting of urethane rubber, silicone rubber, and a thermoplastic resin. 13. The cell culture method according to claim 1, further comprising: removing creases from the culture container placed on the upper surface of the container table using a holder. 14. The cell culture method according to claim 13, wherein the holder holds two sides of the culture container in an extensible part thereof to remove the creases. 15. The cell culture method according to claim 1, further comprising: keeping the culture medium and or the cells being cultured enclosed within the culture container uniform. 16. The cell culture method according to claim 1, wherein a hardness of an outer peripheral surface of the partition roller is at least 90 or smaller when measured by a type A durometer in accordance with JIS K 6253.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (10)
Amiot Bruce P. (Delano MN), Cell culture apparatus.
Porchia Jose (Midland MI) Dais Brian C. (Sanford MI) McBride Karen E. (Indianapolis IN) Farrelly D. Lyn (Indianapolis IN) Steele Robert R. (Indianapolis IN), Flexible thermoplastic containers having a visual pattern thereon.
Taiariol Van (Redwood City CA) Oakley Robert V. (Lafayette CA) Ventura Peter (Daly City CA), Method for in vitro reproduction and growth of cells in culture medium.
Pattillo Martha C. (Evanston IL) Bacehowski David V. (Wildwood IL) Bilstad Arnold C. (Deerfield IL) Cullis Herbert M. (Silver Spring MD) Dennehey T. Michael (Arlington Heights IL) Yang James W. (Scha, System for metering nutrient media to cell culture containers and method.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.