Compositions and devices of poly-4-hydroxybutyrate
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61L-031/10
A61L-027/18
A61L-031/06
C08G-063/06
C12P-007/62
G01N-033/50
A61L-027/34
A61L-017/10
출원번호
US-0529217
(2014-10-31)
등록번호
US-9480780
(2016-11-01)
발명자
/ 주소
Martin, David P.
Williams, Simon F.
출원인 / 주소
Tepha, Inc.
대리인 / 주소
Pabst Patent Group LLP
인용정보
피인용 횟수 :
1인용 특허 :
37
초록▼
Compositions of P4HB with high purity have been developed. The compositions are prepared by washing P4HB biomass prior to solvent extraction, and precipitating P4HB from solution. The same solvent is preferably used to wash the P4HB biomass, and as a non-solvent to precipitate the polymer from a P4H
Compositions of P4HB with high purity have been developed. The compositions are prepared by washing P4HB biomass prior to solvent extraction, and precipitating P4HB from solution. The same solvent is preferably used to wash the P4HB biomass, and as a non-solvent to precipitate the polymer from a P4HB solvent solution. The highly pure P4HB compositions are suitable for preparing implants. The implants may be used for the repair of soft and hard tissues.
대표청구항▼
1. A composition comprising poly-4-hydroxybutyrate (P4HB) polymer, wherein the isotopes of hydrogen, carbon and/or oxygen in the polymer have optionally been enriched and wherein the P4HB polymer is obtained by a process comprising: suspending a P4HB biomass in ethanol for a period of time effective
1. A composition comprising poly-4-hydroxybutyrate (P4HB) polymer, wherein the isotopes of hydrogen, carbon and/or oxygen in the polymer have optionally been enriched and wherein the P4HB polymer is obtained by a process comprising: suspending a P4HB biomass in ethanol for a period of time effective to extract lipids into the ethanol;separating the P4HB biomass from the ethanol by solid-liquid separation;collecting the ethanol-washed biomass; andextracting the P4HB polymer into a solvent. 2. The composition of claim 1 wherein the abundance of deuterium in the polymer exceeds 0.0115% of all elemental hydrogen present in the polymer, and/or the polymer contains tritium. 3. The composition of claim 1 wherein the abundance of carbon-13 in the polymer exceeds 1.07% of all elemental carbon present in the polymer, and/or the polymer contains carbon-14. 4. The composition of claim 1 wherein the abundance of oxygen-17 in the polymer exceeds 0.038% of all elemental oxygen present in the polymer, and/or the abundance of oxygen-18 in the polymer exceeds 0.205%. 5. The composition of claim 1 wherein the abundance of deuterium in the polymer exceeds 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or 85% of the elemental hydrogen present in the polymer. 6. The composition of claim 1 wherein the composition has a lipid content of less than 100 ppm as determined by gas chromatography after butanolysis. 7. The composition of claim 1 wherein the polymer is precipitated with a non-solvent for the polymer, harvested, and dried. 8. The composition of claim 7 wherein the solvent is selected from the group consisting of methylene chloride, chloroform, tetrahydrofuran, acetone, dioxane, ethyl acetate, dimethylene carbonate, dimethyl sulfoxide, dimethyl formamide, methyl ethyl ketone, butyl acetate, butyl propionate, and diethyl carbonate, and the non-solvent is selected from the group consisting of ethanol or aqueous ethanol. 9. The composition of claim 1 wherein the weight average molecular weight of the polymer is from 50,000 to 1.2 million Da. 10. The composition of claim 1 wherein the composition further comprises a therapeutic, diagnostic or prophylactic agent. 11. The composition of claim 1 sterilized with cold ethylene oxide gas, gamma-irradiation, or electron beam irradiation. 12. The composition of claim 1 wherein the composition is used as a coating on a medical device. 13. The composition of claim 12 used for soft or hard tissue repair, regeneration or replacement. 14. The composition of claim 1 wherein the composition forms a fiber wherein the fiber has a tensile strength of greater than 126 MPa. 15. The composition of claim 14 wherein the composition is used to form a yarn wherein the tenacity of the yarn is greater than 0.5 grams per denier. 16. The composition of claim 15 formed into a suture or textile. 17. A method of manufacturing a composition comprising poly-4-hydroxybutyrate (P4HB) polymer, wherein the isotopes of hydrogen, carbon and/or oxygen-in the polymer have optionally been enriched, comprising feeding 1,4-butanediol that has been enriched in an isotope of hydrogen, carbon and/or oxygen to a microorganism comprising a gene encoding a PHA synthase, suspending the P4HB biomass in ethanol for a period of time effective to extract lipids into the ethanol; separating the P4HB biomass from the ethanol by solid-liquid separation;collecting the ethanol-washed biomass; andextracting the P4HB polymer into a solvent. 18. The method of claim 17 wherein the 1,4-butanediol is fully or partially deuterated. 19. A method of monitoring the degradation of poly-4-hydroxybutyrate polymer in vivo, wherein the isotopes of hydrogen, carbon and/or oxygen in the polymer have been enriched and wherein the P4HB polymer is obtained by a process comprising: suspending a P4HB biomass in ethanol for a period of time effective to extract lipids into the ethanol;separating the P4HB biomass from the ethanol by solid-liquid separation;collecting the ethanol-washed biomass; andextracting the P4HB polymer into a solvent,comprising detecting an isotope enriched metabolite of the polymer in a blood or urine sample. 20. The method of claim 19 wherein the metabolite is 4-hydroxybutyrate enriched with isotopes of hydrogen, carbon and/or oxygen. 21. The method of claim 19 wherein the isotope enriched metabolite is detected using mass spectroscopy in the presence of the natural 4-hydroxybutyrate metabolite.
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이 특허에 인용된 특허 (37)
Martin, David P.; Guo, Kai; Williams, Simon F., Compositions and devices of poly-4-hydroxybutyrate.
Williams, Simon F.; Martin, David P.; Gerngross, Tillman; Horowitz, Daniel M., Medical device comprising polyhydroxyalkanoate having pyrogen removed using oxidizing agent.
Martin, David P.; Rizk, Said; Ho, Kicherl; Williams, Simon F., Medical devices containing melt-blown non-wovens of poly-4-hydroxybutyrate and copolymers thereof.
Snell Kristi D. ; Hogan Scott A. ; Sim Sang Jun,KRX ; Sinskey Anthony J. ; Rha Chokyun, Method for controlling molecular weight of polyhydroxyalkanoates.
Williams,Simon F.; Martin,David P.; Gerngross,Tillman; Horowitz,Daniel M., Method for making devices using polyhydroxyalkanoate having pyrogen removed.
Yanagita,Yoshifumi; Ogawa,Noriko; Ueda,Yasuyoshi; Osakada,Fumio; Matsumoto,Keiji, Method of collecting highly pure polyhydroxyalkanoate from microbial cells.
Williams, Simon F.; Martin, David P.; Gerngross, Tillman; Horowitz, Daniel M., Method of forming medical devices having pyrogen removed for in vivo application.
Kurdikar Devdatt L. ; Strauser Fred E. ; Solodar A. John ; Paster Mark D. ; Asrar Jawed, Methods of PHA extraction and recovery using non-halogenated solvents.
Narasimhan,Karunakaran; Noda,Isao; Satkowski,Michael Matthew; Cearley,Angella Christine; Gibson,Michael Steven; Welling,Stanley James, Process for the extraction of polyhydroxyalkanoates from biomass.
Williams Simon F. ; Martin David P. ; Gerngross Tillman ; Horowitz Daniel M., Removing endotoxin with an oxdizing agent from polyhydroxyalkanoates produced by fermentation.
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