초록 ▼

The embodiment described herein are related nanoemulsions comprising botulinum toxins. In one embodiment, the nanoemulsions are prepared by high pressure microfluidization and comprise a particle size distribution exclusively between 10 and 300 nm. The nanoemulsions contemplated by the present inven

The embodiment described herein are related nanoemulsions comprising botulinum toxins. In one embodiment, the nanoemulsions are prepared by high pressure microfluidization and comprise a particle size distribution exclusively between 10 and 300 nm. The nanoemulsions contemplated by the present invention are useful for the cosmetic and medical treatment of muscular contracture states. For example, botulinum toxin may relax facial muscles such that skin wrinkles become smoother and less noticeable. Further, the present invention contemplates a cosmetic formulation that may be self-administered, for example, in the privacy of one's home and without medical supervision.

대표청구항 ▼

1. A topical composition comprising a nanoemulsion comprising oily particles dispersed within an aqueous dispersion medium, wherein the majority of the oily particles have diameters between approximately 10 and approximately 300 nanometers; wherein the nanoemulsion comprises at least one botulinum t

1. A topical composition comprising a nanoemulsion comprising oily particles dispersed within an aqueous dispersion medium, wherein the majority of the oily particles have diameters between approximately 10 and approximately 300 nanometers; wherein the nanoemulsion comprises at least one botulinum toxin, a surfactant, and an oil, wherein the oil and surfactant are present in a ratio between 0.5 and 2.0;wherein the composition is formulated so that the botulinum toxin can penetrate human skin without use of an abrasive or disrupting agent, wherein the abrasive or disrupting agent is selected from the group consisting of: chemical permeation enhancers, mechanical permeation enhancers, electrical permeation enhancers and magnetic permeation enhancers; and,wherein the topical composition is formulated as a cream, a lotion, a gel, an ointment, a spray, a powder, an emollient, or combination thereof, for transdermal administration. 2. The topical composition of claim 1, wherein the percent of oil ranges from 1% to 30% of the nanoemulsion. 3. The topical composition of claim 1, wherein the aqueous dispersion medium is selected from the group consisting of water, saline solution, phosphate buffered saline, short chain alcohols, 5% dextrose, Ringer's solution, lactated Ringer's injection, lactated Ringer's plus 5% dextrose injection, acylated Ringer's injection, and combinations thereof. 4. The topical composition of claim 1, wherein the surfactant is selected from the group consisting of phosphoglycerides; phosphatidylcholines; dipalmitoyl phosphatidylcholine (DPPC); dioleylphosphatidyl ethanolamine (DOPE); dioleyloxypropyltriethylammonium (DOTMA); dioleoylphosphatidylcholine; cholesterol; cholesterol ester; diacylglycerol; diacylglycerolsuccinate; diphosphatidyl glycerol (DPPG); hexanedecanol; fatty alcohols such as polyethylene glycol (PEG); polyoxyethylene-9-lauryl ether; a surface active fatty acid, such as palmitic acid or oleic acid; fatty acids; fatty acid monoglycerides; fatty acid diglycerides; fatty acid amides; sorbitan trioleate glycocholate; sorbitan monolaurate; polysorbate 20; polysorbate 61; polysorbate 66; polysorbate; polysorbate 85; polyoxyethylene monostearate; surfactin; a poloxomer; a sorbitan fatty acid ester such as sorbitan trioleate; lecithin; lysolecithin; phosphatidylserine; phosphatidylinositol; sphingomyelin; phosphatidylethanolamine (cephalin); cardiolipin; phosphatidic acid; cerebrosides; dicetylphosphate; dipalmitoylphosphatidylglycerol; stearylamine; dodecylamine; hexadecyl-amine; acetyl palmitate; glycerol ricinoleate; hexadecyl sterate; isopropyl myristate; tyloxapol; poly(ethylene glycol)5000-phosphatidylethanolamine; poly(ethylene glycol)400-monostearate; phospholipids; synthetic and/or natural detergents having high surfactant properties; deoxycholates; cyclodextrins; chaotropic salts; ion pairing agents; and combinations thereof. 5. The topical composition of claim 1, wherein the botulinum toxin is selected from the group consisting of type A, type B, type C1, type C2, type D, type F, and type G. 6. The topical composition of claim 1, wherein the botulinum toxin is type A botulinum toxin. 7. The topical composition of claim 1, wherein the botulinum toxin is a botulinum toxin complex. 8. The topical composition of claim 7, wherein the botulinum toxin complex comprises a non-toxin hemagglutinin protein and a non-toxic non-hemagglutinin protein. 9. The topical composition of claim 1, wherein the botulinum toxin is incorporated within an albumin matrix. 10. The topical composition of claim 9, wherein the albumin is human albumin. 11. The topical composition of claim 1, wherein the botulinum toxin is a purified botulinum toxin protein or fragment thereof that retains at least one biological activity. 12. The topical composition of claim 1, wherein the botulinum toxin is isolated, or substantially isolated, from other proteins. 13. The topical composition of claim 1, wherein the botulinum toxin is isolated, or substantially isolated, from non-toxin proteins. 14. The topical composition of claim 1, wherein the botulinum toxin is isolated from Clostridium botulinum. 15. The topical composition of claim 1, wherein the botulinum toxin is chemically synthesized. 16. The topical composition of claim 1, wherein the botulinum toxin is produced recombinantly. 17. The topical composition of claim 1, wherein the botulinum toxin is: i) encapsulated within the particles;ii) adsorbed on the surface of the particles; and/oriii) associated with the particle interface. 18. The topical composition of claim 1, wherein the nanoemulsion can penetrate the top layer of skin. 19. The topical composition of claim 18, wherein the top layer of the skin is the surface of the stratum corneum. 20. The topical composition of claim 18, wherein the top layer of the skin includes dermal pores. 21. The topical composition of claim 18, wherein the top layer of the skin includes dermal glands.