초록

The invention provides compositions comprising a carrier comprising a continuous phase of a hydrophobic substance, liposomes, and a polynucleotide, and methods for using such compositions for delivering a polynucleotide to a subject.

대표청구항 ▼

1. An injectable composition comprising: a carrier comprising mannide oleate and a continuous phase of mineral oil;liposomes comprising an unsaturated phospholipid; anda polynucleotide encapsulated within said liposomes, said polynucleotide encoding a polypeptide and being operably linked to a promo

1. An injectable composition comprising: a carrier comprising mannide oleate and a continuous phase of mineral oil;liposomes comprising an unsaturated phospholipid; anda polynucleotide encapsulated within said liposomes, said polynucleotide encoding a polypeptide and being operably linked to a promoter functional in mammalian cells; wherein said carrier and said liposomes are present in a ratio of 1:1 by volume. 2. The composition according to claim 1, wherein the composition is a water-in-oil emulsion. 3. The composition according to claim 1, wherein the liposomes comprise cholesterol. 4. The composition of claim 1, wherein the liposomes comprise dioleoyl phosphatidylcholine (DOPC) and cholesterol. 5. The composition according to claim 1, further comprising an adjuvant. 6. The composition according to claim 1, wherein said polynucleotide is inserted in an expression plasmid. 7. The composition according to claim 1, comprising a bacterial or viral vector containing said polynucleotide. 8. The composition according to claim 1, wherein said polynucleotide is present both encapsulated within said liposomes and exterior to said liposomes. 9. The composition according to claim 5, wherein said adjuvant is present: (a) within said liposomes;(b) exterior to said liposomes; or(c) both within said liposomes and exterior to said liposomes. 10. A kit, comprising: a composition of claim 1; and instructions for using said composition to deliver a polynucleotide to a subject. 11. A method for delivering a polynucleotide to a subject, comprising administering the composition of claim 1 to said subject. 12. The method according to claim 11, wherein said subject is a mammal. 13. The method according to claim 12, wherein said mammal is a human. 14. A method for making a composition comprising: (a) providing a polynucleotide that encodes a polypeptide and that is operably linked to a promoter functional in mammalian cells;(b) encapsulating said polynucleotide in liposomes comprising an unsaturated phospholipid; and(c) combining said liposomes with a carrier comprising mannide oleate and a continuous phase of mineral oil in a ratio of 1:1 by volume. 15. The method according to claim 14, wherein the liposomes are dehydrated before they are combined with said carrier. 16. A composition produced by the method according to claim 14. 17. The composition according to claim 1, wherein said polypeptide is an antigen, an antibody or antibody fragment, an enzyme, a cytokine, a therapeutic protein, a chemokine, a regulatory protein, a structural protein, a chimeric protein, a nuclear protein, a transcription factor, a viral protein, a TLR protein, an interferon regulatory factor, an angiostatic or angiogenic protein, an apoptotic protein, an Fc gamma receptor, a hematopoietic protein, a tumor suppressor, a cytokine receptor, or a chemokine receptor. 18. The method according to claim 11, wherein said polypeptide is an antigen, an antibody or antibody fragment, an enzyme, a cytokine, a therapeutic protein, a chemokine, a regulatory protein, a structural protein, a chimeric protein, a nuclear protein, a transcription factor, a viral protein, a TLR protein, an interferon regulatory factor, an angiostatic or angiogenic protein, an apoptotic protein, an Fc gamma receptor, a hematopoietic protein, a tumor suppressor, a cytokine receptor, or a chemokine receptor. 19. The method according to claim 14, wherein said polypeptide is an antigen, an antibody or antibody fragment, an enzyme, a cytokine, a therapeutic protein, a chemokine, a regulatory protein, a structural protein, a chimeric protein, a nuclear protein, a transcription factor, a viral protein, a TLR protein, an interferon regulatory factor, an angiostatic or angiogenic protein, an apoptotic protein, an Fc gamma receptor, a hematopoietic protein, a tumor suppressor, a cytokine receptor, or a chemokine receptor.