A scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning senor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or mo
A scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning senor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, and at least one adapter configured to couple with the substrate and provide optical communication between the light source, the waveguides of the substrate, and the detector.
대표청구항▼
1. A scanning sensing method comprising: coupling a removable substrate comprising a plurality of in-coupling waveguides, a plurality of out-coupling waveguides and combiners for coupling the in-coupling and out-coupling waveguides, wherein the substrate is coupled in optical communication with an a
1. A scanning sensing method comprising: coupling a removable substrate comprising a plurality of in-coupling waveguides, a plurality of out-coupling waveguides and combiners for coupling the in-coupling and out-coupling waveguides, wherein the substrate is coupled in optical communication with an adapter, a light source, and a detector to provide a scanning sensor system, wherein the adapter is coupled to the light source and the detector;delivering a sample suspected of containing a biologically active analyte to be detected to an optical sensing site of the substrate, wherein the optical sensing site comprises a well;providing a first light wave using a light source to one or more of the plurality of in-coupling waveguides of the substrate, wherein the in-coupling waveguides are in optical communication with the optical sensing site, wherein the first light wave is transducable by a sensor associated with the optical sensing site to a second light wave carried to an out-coupling waveguide; anddetecting the second light wave using the detector, wherein a measurable change in the first light wave as measured in the second light wave occurs when the sensor interacts with the biologically active analyte. 2. The method of claim 1, wherein scanning sensing further comprises switching one or more input light wave from the light source into the substrate to produce the first light wave in one or more of the in-coupling waveguides. 3. The method of claim 1, wherein the light source comprises an optical switch for controlled switching of one or more input light wave, the optical switch can multicast light to a plurality of outputs and into the substrate to controllably produce the first light wave in one or more of the in-coupling waveguides. 4. The method of claim 1, wherein the light source comprises an array of individually controlled light generators for controlled switching of one or more input light wave, to controllably produce the first light wave in one or more of the in-coupling waveguides. 5. The method of claim 1, wherein the incoupling waveguides are in optical communication with a plurality of optical sensing sites and the first wave generates a plurality of second light waves from the plurality of optical sensing sites, the method further comprising simultaneously detecting the plurality of second light waves with the detector wherein the detector comprises a photodetector array. 6. The method of claim 1, wherein, a portion of the sensing sites comprise reference sample material for calibration and/or normalization. 7. The method of claim 1, wherein the biologically active analyte is selected from the group consisting of a nucleic acid, a protein, an antigen, an antibody, a lipid, a polysaccharide, a cell, a tissue, a microorganism, a gas, a chemical agent and a pollutant. 8. The method of claim 1, wherein the biologically active analyte is a protein. 9. The method of claim 1, wherein a SNP is detected in the biologically active analyte. 10. The method of claim 1, wherein a marker for gene expression is detected in the biologically active analyte. 11. The method of claim 1, wherein the sensor comprises an immunoassay reagent that supports an immunoassay and wherein the immunoassay reagent of the sensor interacting with the biologically active analyte comprises an outcome of an immunoassay. 12. The method of claim 11, wherein the immunoassay supported is an enzyme-linked immunosorbent assay (ELISA). 13. The method of claim 11, wherein the immunoassay supported is a fluorescent immunoassay. 14. The method of claim 1, wherein detecting a measurable change in the first lightwave as measured in the second lightwave provides a diagnostic result. 15. The method of claim 1, further comprising conducting a real-time PCR reaction at the optical sensing site. 16. A chip for detecting a biologically active analyte, comprising a substrate wherein the substrate comprises a plurality of in-coupling waveguides coupled to a plurality of out-coupling waveguides by combiners, where the out-coupling waveguides are in optical communication with a plurality of sensing sites. 17. The chip of claim 16, wherein the optical sensing sites comprise a sensor and a sample comprising a biologically active analyte, and wherein a measurable change in a first light wave results when the sensor discriminates or interacts with the biologically active analyte. 18. The chip of claim 16 wherein a first light wave in an in-coupling waveguide is transduced by a sensor of an optical sensing site in optical communication with the out-coupling waveguide resulting in a second light wave in a out-coupling waveguide. 19. The chip of claim 17, wherein the sensor comprises an immunoassay reagent that supports an immunoassay. 20. The chip of claim 19, wherein the immunoassay supported is an enzyme-linked immunosorbent assay (ELISA). 21. The chip of claim 19, wherein the immunoassay supported is a fluorescent immunoassay. 22. The chip of claim 17, wherein the sensor is selected from the group consisting of a fluorescence well, an absorption cell, an interferometric sensor, a diffractive sensor and surface plasmon resonance sensor. 23. The chip of claim 16, wherein the biologically active analyte is selected from the group consisting of a nucleic acid, a protein, an antigen, an antibody, a lipid, a polysaccharide, a glycoprotein, a cell, a tissue, a microorganism, a gas, a chemical agent and a pollutant. 24. The chip of claim 23, wherein the nucleic acid is produced via an amplification reaction. 25. The chip of claim 16, wherein the in-coupling waveguides are single-mode and the out-coupling waveguides are multi-mode. 26. The chip of claim 16, wherein the in-coupling waveguides and the out-coupling waveguides support single-mode in a first vertical dimension and multi-mode in a second lateral dimension. 27. The chip of claim 16, wherein the in-coupling waveguides and the out-coupling waveguides are multi-mode. 28. The chip of claim 16, wherein the in-coupling waveguides and the out-coupling waveguides are single-mode. 29. The chip of claim 16, wherein the in-coupling waveguide comprises a plurality of branches for drawing a fraction of the light from a first light wave traveling in the in-coupling waveguide. 30. The chip of claim 29, wherein the in-coupling waveguide branches are in optical communication with the in-coupling waveguide. 31. The chip of claim 16, wherein the out-coupling waveguide comprises a plurality of funnels for collecting light from the sensing sites and coupling it to the out-coupling waveguide. 32. The chip of claim 16, wherein the optical sensing sites comprise wells. 33. The chip of claim 16, wherein the optical sensing sites comprise the surface of the substrate above the out-coupling waveguides. 34. The chip of claim 16, wherein the optical sensing sites comprise biochemical interaction sites. 35. The chip of claim 16, wherein the optical sensing sites comprise optical transducers. 36. The chip of claim 35, wherein the optical transducers comprise wells comprising fluorescent compounds, wherein light waves guided by the in-coupling waveguides excite the fluorescent compound in the wells in the presence of a biologically active analyte, and the out-coupling waveguides collect and guide light emitted from the fluorescent compound or the luminescent compounds in the wells to the detector. 37. The chip of claim 16, wherein the number of sensing wells is greater than 10. 38. The chip of claim 16, wherein the density of sensing wells is greater than 100 per cm2. 39. The chip of claim 16, wherein the chip is in thermal communication with a thermal transfer element. 40. The chip of claim 39, wherein the thermal transfer element is a thermoelectric cooler. 41. The chip of claim 16, wherein each optical sensing site comprises a thermal transfer element in thermal communication with the optical sensing site. 42. The chip of claim 41, wherein the thermal transfer element comprises a thin-film heater. 43. The chip of claim 41, wherein each optical sensing site further comprises a thermistor in thermal communication with the optical sensing site. 44. The chip of claim 16, wherein the substrate further comprises one or more microchannel and one or more reservoirs in fluid communication with one or more optical sensing site. 45. The chip of claim 16, wherein the chip further comprises a fluidics layer coupled to the substrate and comprising one or more microchannel and one or more reservoirs in fluid communication with one or more optical sensing site.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (148)
Haugland Richard P. (Eugene OR) Gee Kyle R. (Springfield OR), a
상세보기
Kim Yeong-Ju,KRX ; Park Chan-Sik,KRX, Aligning apparatus for optical coupling and manufacturing method thereof.
Metzker Michael L. ; Gibbs Richard A., Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis.
Herron James N. (Salt Lake City UT) Christensen Douglas A. (Salt Lake City UT) Wang Hsu-Kun (Salt Lake City UT) Caldwell Karin D. (Salt Lake City UT) Janatova Vera (Prague CSX) Huang Shao-Chie (Salt , Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays.
Herron James N. ; Christensen Douglas A. ; Wang Hsu-Kun ; Caldwell Karin ; Janatova Vera,CZX ; Huang Shao-Chie, Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays.
Herron James N. ; Christensen Douglas A. ; Wang Hsu-Kun ; Caldwell Karin D. ; Janatova Vera,CZX ; Huang Shao-Chie, Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays.
Chee Mark ; Gingeras Thomas R. ; Fodor Stephen P. A. ; Hubble Earl A. ; Morris MacDonald S., Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same.
Cronin Maureen T. ; Miyada Charles Garrett ; Hubbell Earl A. ; Chee Mark ; Fodor Stephen P. A. ; Huang Xiaohua C. ; Lipshutz Robert J. ; Lobban Peter E. ; Morris Macdonald S. ; Sheldon Edward L., Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis.
Gingeras Thomas R. ; Mack David ; Chee Mark S. ; Berno Anthony J. ; Stryer Lubert ; Ghandour Ghassan ; Wang Ching, Chip-based species identification and phenotypic characterization of microorganisms.
Khoe Giok D. (Eindhoven NLX) Van Leest Johannes H. F. M. (Eindhoven NLX) Meuleman Lambertus J. (Eindhoven NLX), Connector for coupling at least one optical fiber to a further optical element.
Abel, Andreas Peter; Duveneck, Gert Ludwig; Ehrat, Markus; Kresbach, Gerhard Matthias; Pawlak, Michael; Schürmann-Mader, Eveline, Device and method for determining multiple analytes.
Bischel William K. ; Brinkman Michael J. ; Deacon David A. G. ; De Wath Edward J. ; Dyer Mark J. ; Field Simon J., Display architecture with waveguide routing and out-plane emission.
Gerdt David W. (P.O. Box 8175 Charlottesville VA 22906) Herr John C. (P.O. Box 8175 Charlottesville VA 22906), Fiber optic evanscent wave sensor for immunoassay.
Schmidt,Holger; Hawkins,Aaron Roe; Deamer,David W., Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity.
Prucnal Paul R. (Bardonia NY) Fossum Eric R. (Peekskill NY) Osgood ; Jr. Richard M. (Chappaqua NY), Integrated fiber optic coupler for VHSIC/VLSI interconnects.
Ridgway Richard W. (Westerville OH) Boiarski Anthony A. (Columbus OH) Wood Van E. (Delaware OH) Busch James R. (Columbus OH), Integrated optical compensating refractometer apparatus.
Chande Tushar S. (Schenectady NY) Jones Marshall G. (Scotia NY) Ortiz ; Jr. Angel L. (Ballston Lake NY) August ; Jr. John L. (Schenectady NY), Laser beam directing system.
Dorn,Ingmar; Bieringer,Thomas; Hagen,Rainer; Kostromine,Serguei; Kunz,Rino E., Layer structure and optical waveguide sensor based on photoaddressable polymers.
Gerolf Kraus DE; Michael Pawlak DE; Gert Ludwig Duveneck DE; Peter Oroszlan CH; Andreas Helg CH; Alfredo Emilio Bruno-Raimondi CH, Measuring device and the use thereof.
John R. Stuelpnagel ; Mark S. Chee ; Richard J. Pytelewski ; Todd Alan Dickinson ; Gan G. Wang, Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems.
Lukosz Walter (Burstwiesenstr. 55 Greifensee CHX 8606), Method and apparatus for selecting detection of changes in samples by integrated optical interference.
Armiento Craig A. (Acton MA) Jagannath Chirravuri (Medfield MA) Tabasky Marvin J. (West Peabody MA) Fitzgerald Thomas W. (Framingham MA) Lockwood Harry F. (Waban MA) Haugsjaa Paul O. (Acton MA) Rothm, Method and device for passive alignment of diode lasers and optical fibers.
Dan Haronian IL; Menachem Nathan IL; Jonathan M. Gershoni IL; Arieh Yaron IL, Method, chip, device and system for effecting and monitoring nucleic acid accumulation.
Shieh Chan-Long (Paradise Valley AZ) Ackley Donald E. (Lambertville NJ) Maracas George N. (Phoenix AZ) Harvey ; III Thomas B. (Scottsdale AZ), Molecular detection apparatus and method using optical waveguide detection.
Groger Howard P. (Gainesville FL) Lo Peter (Blacksburg VA) Churchill Russell J. (Radford VA) Weiss Martin (New Port Richey FL) Luo Shufang (Blacksburg VA), Optical chemical sensor and method using same employing a multiplicity of fluorophores contained in the free volume of a.
Roth Christoph (Saitama JPX) Liu Yuan (Kawagoe JPX) Prass Werner (Mainz DEX) Yamamoto Tetsu (Kawagoe JPX) Motosugi Kenji (Kawagoe JPX), Optical sensor for detection of chemical species.
Tiefenthaler Kurt (Zrich CHX) Lukosz Walter (Greifensee CHX), Optical sensor for selective detection of substances and/or for the detection of refractive index changes in gaseous, li.
Asawa Charles K. ; Asawa Mike H. ; Asawa Jane K. ; Asawa Michi, Optical waveguide including singlemode waveguide channels coupled to a multimode fiber.
Luttermann, Klaus; Diessel, Edgar; Kosch, Winfried; Weichel, Walter, Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand.
Riehle,Claus; Brand,Marcus; Hilgers,Frank; J?ger,Klaus; Giesen,Rainer; Hamacher,Heinz Josef; Wolf,Udo, Process for monitoring and controlling nitrating processes with the aid of an online spectrometer.
Gassen Karl-Rudolf (Odenthal DEX), Process for the preparation of a
상세보기
Podszun Wolfgang (Koeln DEX) Mller Michael (Bergisch Gladbach DEX) Cramer Wilfried (Leverkusen DEX) Rehbold Bodo (Koeln DEX), Process for the production of mouldings of crosslinked polymers.
Alfredo Emilio Bruno-Raimondi CH; Reinhard Volkel CH; Gert Ludwig Duveneck DE; Carlo Stefan Effenhauser DE; Hans-Peter Herzig CH; Rene Dandliker CH, Sensing unit provided with separated detection light guiding.
Neuschafer Dieter,CHX ; Duveneck Gert Ludwig,DEX ; Pawlak Michael,DEX ; Pieles Uwe,DEX ; Budach Wolfgang,CHX, Sensor platform and method for the parallel detection of a plurality of analytes using evanescently excited luminescence.
Leimann Gerhard (Solingen DEX) Paul Friedemann (Bergisch Gladbach DEX) Kamps Rainer (Grub am Forst DEX) Lange Gerhard (Munich DEX) Pfandl Walter (Ahorn DEX), Sheathing material for optical fibers, based on polyalkylene terephthalate/polycarbonate.
Tessier Theodore G. (Crystal Lake IL) Lindsey Scott (Carpentersville IL), Silicon etching process using polymeric mask, for example, to form V-groove for an optical fiber coupling.
Herron James N. (Salt Lake City UT) Christensen Douglas A. (Salt Lake City UT) Caldwell Karin D. (Salt Lake City UT) Janatov Vera (Prague UT CSX) Huang Shao-Chie (Salt Lake City UT) Wang Hsu-Kun (Sal, Waveguide immunosensor with coating chemistry providing enhanced sensitivity.
Maisenhoelder, Bernd; Edlinger, Johannes; Heine-Kempkens, Claus; Pawlak, Michael; Duveneck, Gert, Waveguide plate and process for its production and microtitre plate.
Flanagan Michael T. (Bishop\s Stortford GB2) Sloper Andrew N. (London GB2), Waveguide sensor with input and reflecting gratings and its use in immunoassay.
Dahne Claus (Onex CHX) Bregnard Andr (Le Lignon CHX) Revillet Georges (Onex CHX) Sutherland Ranald M. (Carouge CHX), Waveguide to be used as optical probe in multiple internal reflection spectroscopic analysis.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.