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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0248991 (2014-04-09) |
등록번호 | US-9534216 (2017-01-03) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 1 인용 특허 : 461 |
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
1. A method for detecting a target nucleic acid fragment from a sample, the method comprising: providing a sample comprising a plurality of different nucleic acid fragments;partitioning the sample into a plurality of droplets, wherein each of the plurality of droplets comprises one or more of the di
1. A method for detecting a target nucleic acid fragment from a sample, the method comprising: providing a sample comprising a plurality of different nucleic acid fragments;partitioning the sample into a plurality of droplets, wherein each of the plurality of droplets comprises one or more of the different nucleic acid fragments, reagents for an amplification reaction, and one or more different optically labeled probes specific for one or more different nucleic acid fragments;amplifying the different nucleic acid fragments in a plurality of droplets;flowing the plurality of droplets comprising the different amplified nucleic acid fragments past a detector that detects polarized light;detecting a level of polarized light emitted from each of the plurality of dropletscomprising the different amplified nucleic acid fragments as each of the plurality of droplets flows past the detector;comparing the detected polarized light emitted from each of the plurality of droplets, comprising the different amplified nucleic acid fragments from the different portions of the sample in each of the plurality of droplets, to one another; andidentifying the different amplified nucleic acid fragments from the different portions of the sample in one or more of the plurality of droplets in order to detect the different amplified nucleic acid fragments. 2. The method of claim 1, wherein a high level of polarized light is indicative of an absence of the target nucleic acid fragment. 3. The method of claim 1, wherein a low level of polarized light is indicative of a positive presence of the target nucleic acid fragment. 4. The method of claim 1, wherein the one or more different optically labeled probes each comprise a fluorescent label. 5. The method of claim 1, wherein the one or more different optically labeled probes each further comprise a quencher molecule. 6. The method claim 1, wherein the probe further comprises a moiety that alters polarization of light emitted from each of the plurality of droplets. 7. The method of claim 1, wherein the sample is a human tissue or body fluid. 8. The method of claim 1, wherein each of the plurality of droplets comprises one nucleic acid fragment. 9. A method for detecting a target nucleic acid from a sample, the method comprising: providing a sample comprising a plurality of different nucleic acid fragments;partitioning the sample into a plurality of droplets, wherein each of the plurality of droplets comprises one or more of the different nucleic acid fragments, reagents for an amplification reaction, and one or more different detectably labeled probes specific for one or more different nucleic acid fragments;amplifying the different nucleic acid fragments in a plurality of droplets; configuring a polarization filter with respect to an excitation polarization; flowing the plurality of droplets comprising the different amplified nucleic acid fragments past a detector that comprises the polarization filter;detecting a level of polarization light emitted from each of the plurality of droplets comprising the different amplified nucleic acid fragments as each of the plurality of droplets flows past the detector;comparing the detected polarized light emitted from each of the plurality of droplets, comprising the different amplified nucleic acid fragments from the different portions of the sample in each of the plurality of droplets, to one another; andidentifying the different amplified nucleic acid fragments from the different portions of the sample in one or more of the plurality of droplets in order to detect the different amplified nucleic acid fragments. 10. The method of claim 9, wherein the detectable label is a fluorescent label. 11. The method of claim 10, wherein the detector is configured such that the polarization filter is perpendicular to the excitation polarization. 12. The method of claim 11, wherein a high level of polarized light is indicative of an absence of the target nucleic acid fragment. 13. The method of claim 11, wherein a low level of polarized light is indicative of a positive presence of the target nucleic acid fragment. 14. The method of claim 9, wherein the probe further comprises a quencher molecule. 15. The method claim 9, wherein the plurality of droplets each comprise one nucleic acid fragment. 16. The method of claim 10, wherein the detector is configured such that the polarization filter is parallel to the excitation polarization.
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