Fungal endophytes for improved crop yields and protection from pests
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-063/04
A01H-005/10
출원번호
US-0964440
(2015-12-09)
등록번호
US-9545111
(2017-01-17)
발명자
/ 주소
Sword, Gregory A.
출원인 / 주소
The Texas A & M University System
대리인 / 주소
Fenwick & West LLP
인용정보
피인용 횟수 :
8인용 특허 :
36
초록▼
The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of
The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.
대표청구항▼
1. A method for improving a trait in a cotton plant, the method comprising: contacting a cotton a seed of said cotton plant with a formulation comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are Dothideo
1. A method for improving a trait in a cotton plant, the method comprising: contacting a cotton a seed of said cotton plant with a formulation comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are Dothideomycetes capable of producing substances that are beneficial to plants or detrimental to pests or both, and are present in the formulation in an amount effective to decrease the colonization frequencies of endophytes of genus Alternaria that are native to the cotton plant and to provide a benefit to the cotton plant compared to a reference cotton plant grown from a seed untreated with the Dothideomycetes facultative fungal endophytes, wherein the benefit is selected from the group consisting of increased square retention, increased boll retention, increased biomass, increased root length, increased root mass, enhanced resistance to drought stress and increased yield. 2. The method of claim 1, wherein the increased square retention is measured in first fruiting position on branches as flowers begin to develop. 3. The method of claim 1, wherein the increased biomass is measured at mid-season in a field. 4. The method of claim 1, wherein the enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt or time to death as compared to a seedling grown from a reference seed. 5. The method of claim 1, wherein yield is increased by at least about 2%. 6. The method of claim 1, wherein the formulation contains at least 100 (10^2) spores/ml or 100,000 (10^5) spores/g dry weight of the facultative fungal endophytes. 7. The method of claim 1, wherein the benefit is increased square retention. 8. The method of claim 1, wherein the benefit is increased boll retention. 9. The method of claim 1, wherein the benefit is increased biomass. 10. The method of claim 1, wherein the benefit is increased root length. 11. The method of claim 1, wherein the benefit is increased root mass. 12. The method of claim 1, wherein the benefit is enhanced resistance to drought stress. 13. The method of claim 1, wherein the benefit is increased yield. 14. A synthetic combination of a cotton seed and purified filamentous, spore-forming facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are Dothideomycetes capable of producing substances that are beneficial to plants or detrimental to pests or both and are present in an amount effective to decrease the colonization frequencies of endophytes of genus Alternaria that are native to the cotton plant grown from the seed and to provide a benefit to the cotton plant grown from the seed compared to a reference cotton plant grown from a seed untreated with the Dothideomycetes facultative fungal endophytes, wherein the benefit is selected from the group consisting of increased square retention, increased boll retention, increased biomass, increased root length, increased root mass, enhanced resistance to drought stress and increased yield. 15. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present in an amount effective to provide the benefit of increased yield by about 2%. 16. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present at a concentration of at least 100 (10^2) spores/seed on the surface of the seed. 17. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present at a concentration of at least 1,000 (10^3) spores/seed on the surface of the seed. 18. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present at a concentration of at least 10,000 (10^4) spores/seed on the surface of the seed. 19. The synthetic combination of claim 14, wherein the facultative fungal endophytes are in spore form. 20. The synthetic combination of claim 14, comprising at least 2 species of facultative endophytes. 21. The synthetic combination of claim 14, wherein the facultative fungal endophyte is native to the cotton plant grown from the seed.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (36)
Beaujot, Patrick M., Air seeder/fertilizer apparatus having metering means and distribution manifold with selectively openable ports.
Isawa, Tsuyoshi; Yasuda, Michiko; Shinozaki, Satoshi; Nakashita, Hideo; Kudo, Toshiaki, Bacteria and method for controlling plant disease using the same.
Henson, Joan M.; Sheehan, Kathy B.; Rodriguez, Russell J.; Redman, Regina S., Curvularia strains and their use to confer stress tolerance and/or growth enhancement in plants.
Leps Walter T. (3943 - 55 Street T6L 1C1 Edmonton ; Alberta CAX) Thompson Bradley G. (39 - 3115 - 119 Street T6J 5N5 Edmonton ; Alberta CAX), Method of adhering bacteria to seed and composition therefor.
Stritzker, Jochen Harald; Hill, Phil; Szalay, Aladar A.; Yu, Yong A., Methods and compositions for detection of microorganisms and cells and treatment of diseases and disorders.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
Imada,Takahiro; Hiruma,Naoya; Isawa,Tsuyoshi; Noda,Munehiro; Kurihara,Yohsuke; Kon,Madoka, Pest control method for grass family plants using endophytic bacteria, pest control material, and seed bound to the pest control material.
Hurley Richard H. (Flemington NJ) Funk ; Jr. Cyril R. (East Brunswick NJ), Plants having endophytio-fungus-enhanced performance and method of producing same.
Nielsen Sven-Erik (Trekanten 5 Hinnerup DKX) Sorensen Grete Morch (Trekanten 5 Hinnerup DKX DK-8382), Rhizobia transformants which symbiotically fixes nitrogen in non-legumes, a material for treating seeds of a non-legume.
Isawa,Tsuyoshi; Hiruma,Naoya; Imada,Takahiro; Noda,Munehiro; Kurihara,Yohsuke; Kon,Madoka, Rice containing endophytic bacteria and method of producing it.
Gao, Ai-Guo; Kolacz, Kathryn H.; MacRae, Ted C.; Miklos, John A.; Paradise, Mark S.; Perlak, Frederick J.; Dressel Toedebusch, Andrea S., Soybean plant and seed corresponding to transgenic event MON87701 and methods for detection thereof.
Denisov, Gennady A.; Arehart, Alan B.; Curtin, Michael D., System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms.
David M Tricoli ; Kim J. Carney ; Paul F. Russell ; Hector D. Quemada ; Russell J. McMaster ; John F. Reynolds ; Rosaline Z. Deng, Transgenic plants expressing DNA constructs containing a plurality of genes to impart virus resistance.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.