Composition for collecting placental stem cells and methods of using the composition
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-009/00
C12N-005/073
출원번호
US-0648812
(2006-12-28)
등록번호
US-9598669
(2017-03-21)
발명자
/ 주소
Edinger, James W.
Heidaran, Mohammad
Hofgartner, Wolfgang
출원인 / 주소
Anthrogenesis Corporation
대리인 / 주소
Jones Day
인용정보
피인용 횟수 :
0인용 특허 :
43
초록▼
The present invention provides improved compositions and methods for the collection of stem cells from an organ, e.g., placenta. The invention provides a stem cell collection composition comprising an apoptosis inhibitor and, optionally, an enzyme such as a protease or mucolytic enzyme, vasodilator,
The present invention provides improved compositions and methods for the collection of stem cells from an organ, e.g., placenta. The invention provides a stem cell collection composition comprising an apoptosis inhibitor and, optionally, an enzyme such as a protease or mucolytic enzyme, vasodilator, necrosis inhibitor, oxygen-carrying perfluorocarbon, or an organ preserving compound. The invention provides methods of using the stem cell collection composition to collect stem cells and to preserve populations of stem cells.
대표청구항▼
1. A composition comprising, in a physiologically-acceptable solution, a JNK inhibitor, wherein said JNK inhibitor is 3-(4-fluoro-phenyl)-1H-indazole-5-carboxylic acid amide; a protease; an oxygen-carrying perfluorocarbon; 3-(4-amino-1-oxo-1,3-dihydroisoindol-2-yl)-piperidine-2,6-dione; atrial natri
1. A composition comprising, in a physiologically-acceptable solution, a JNK inhibitor, wherein said JNK inhibitor is 3-(4-fluoro-phenyl)-1H-indazole-5-carboxylic acid amide; a protease; an oxygen-carrying perfluorocarbon; 3-(4-amino-1-oxo-1,3-dihydroisoindol-2-yl)-piperidine-2,6-dione; atrial natriuretic peptide (ANP); and either pyrrolidine dithiocarbamate or clonazepam; wherein said composition is not a naturally-occurring composition, and wherein said JNK inhibitor is an inhibitor of apoptosis; andwherein the composition is suitable for collecting placental stem cells. 2. The composition of claim 1, wherein said protease is present in an amount sufficient to detectably dissociate cells of a tissue comprising stem cells. 3. The composition of claim 1 wherein said physiologically-acceptable solution is a saline solution or culture medium. 4. The composition of claim 3 wherein said saline solution is 0.9% NaCl solution or phosphate buffered saline. 5. The composition of claim 1, wherein said protease is a matrix metalloprotease or a neutral protease. 6. The composition of claim 5, wherein said matrix metalloprotease is collagenase. 7. The composition of claim 5, wherein said neutral protease is thermolysin or dispase. 8. The composition of claim 1, wherein said composition comprises hyaluronidase. 9. The composition of claim 1, additionally comprising hydroxyethyl starch, lactobionic acid and raffinose. 10. The composition of claim 1, additionally comprising UW solution. 11. The composition of claim 1, wherein said JNK inhibitor is an indazole. 12. The composition of claim 1 comprising a plurality of stem cells. 13. The composition of claim 12, wherein said stem cells comprise CD34+ stem cells. 14. The composition of claim 13, wherein said CD34+ stem cells comprise CD34+CD38− stem cells. 15. The composition of claim 13, wherein said CD34+CD38− stem cells are part of a population of CD34+CD38− stem cells present in placental perfusate as a higher percentage of total nucleated cells as compared to the percentage of CD34+CD38− cells in cord blood. 16. The composition of claim 12, wherein said stem cells are CD34− stem cells. 17. The composition of claim 16, wherein said CD34− stem cells are additionally HLA-G+ or CD200+. 18. The composition of claim 16, wherein said CD34− stem cells are additionally OCT-4+, CD73+, or CD105+.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (43)
Hon-Wah Man ; George W. Muller, 1-oxo-and 1,3-dioxoisoindolines.
Kois,Adam; MacFarlane,Karen J.; Satoh,Yoshitaka; Bhagwat,Shripad S.; Parnes,Jason S.; Palanki,Moorthy S. S.; Erdman,Paul E., Anilinopyrimidine derivatives as IKK inhibitors and compositions and methods related thereto.
Kois,Adam; MacFarlane,Karen J.; Satoh,Yoshitaka; Bhagwat,Shripad S.; Parnes,Jason S.; Palanki,Moorthy S. S.; Erdman,Paul E., Anilinopyrimidine derivatives as IKK inhibitors and compositions and methods related thereto.
Heidaran, Mohammad; Wang, Jia-Lun; Ye, Qian; Zeitlin, Andrew; Dulaney, Colleen Suzanne, Co-culture of placental stem cells and stem cells from a second source.
Bhagwat,Shripad S.; Satoh,Yoshitaka; Sakata,Steven T.; Buhr,Chris A.; Albers,Ronald; Sapienza,John; Plantevin,Veronique; Chao,Qi; Sahasrabudhe,Kiran; Ferri,Rachel, Indazole compounds and compositions thereof as JNK inhibitors and for the treatment of diseases associated therewith.
Bhagwat,Shripad S.; Satoh,Yoshitaka; Sakata,Steven T.; Buhr,Chris A.; Albers,Ronald; Sapienza,John; Plantevin,Veronique; Chao,Qi; Sahasrabudhe,Kiran; Ferri,Rachel, Indazole compounds and compositions thereof as JNK inhibitors and for the treatment of diseases associated therewith.
Sakata,Steven T.; Raymon,Heather K., Isothiazoloanthrones, isoxazoloanthrones, isoindolanthrones and derivatives thereof a JNK inhibitors and compositions and methods related thereto.
Sakata,Steven T.; Raymon,Heather K., Isothiazoloanthrones, isoxazoloanthrones, isoindolanthrones and derivatives thereof as JNK inhibitors and compositions and methods related.
Hariri,Robert J.; Stirling,David I.; Moutouh De Parseval,Laure A.; Chan,Kyle W. H., Modulation of stem and progenitor cell differentiation, assays, and uses thereof.
Bennett,Brydon L.; Bhagwat,Shripad S.; Manning,Anthony M.; Murray,Brion W.; O'Leary,Eoin C.; Satoh,Yoshitaka, Pyrazoloanthrone and derivatives thereof as JNK inhibitors and compositions and methods related thereto.
Stern David M. (Great Neck NY) Oz Mehmet C. (Fort Lee NJ) Nowygrod Roman (Teaneck NJ) Koga Shin (New York NY) Pinsky David J. (Riverdale NY), Solution for prolonged organ preservation.
Muller George W. ; Stirling David I. ; Chen Roger Shen-Chu ; Man Hon-Wah, Substituted 2-(2,6 dioxo-3-fluoropiperidin-3-yl)-isoindolines and method of reducing TNF.alpha. levels.
Muller George W. ; Stirling David I. ; Chen Roger Shen-Chu ; Man Hon-Wah, Substituted 2-(2,6-dioxo-3-fluoropiperidin-3-yl)-isoindolines and method of reducing inflammatory cytokine levels.
George W. Muller ; David I. Stirling ; Roger Shen-Chu Chen, Substituted 2-(2,6-dioxopiperidin-3-yl)- phthalimides and 1-oxoisoindolines and method of reducing TNF.alpha. levels.
Muller George W. ; Stirling David I. ; Chen Roger Shen-Chu, Tetrasubstituted 2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolines and method of reducing TNF.alpha. levels.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.