최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0873818 (2007-10-17) |
등록번호 | US-9598723 (2017-03-21) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 0 인용 특허 : 348 |
An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample
An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.
1. An automated analyzer for use in performing a nucleic acid-based assay, the analyzer comprising: a separation station comprising a magnetic element for subjecting a sample to a magnetic field, the magnetic field being sufficient to isolate a target nucleic acid immobilized on a solid support comp
1. An automated analyzer for use in performing a nucleic acid-based assay, the analyzer comprising: a separation station comprising a magnetic element for subjecting a sample to a magnetic field, the magnetic field being sufficient to isolate a target nucleic acid immobilized on a solid support comprising a magnetically-responsive particle from other material present in the sample, wherein the magnetic element is carried by a magnet moving structure configured to be automatically movable between first and second positions, wherein the magnetic element does not have a substantial effect on the magnetically-responsive particle when the magnet moving structure is in the first position, and wherein the magnetic element has a substantial effect on the magnetically-responsive particle when the magnet moving structure is in the second position;an amplification incubator adapted to receive a receptacle containing the target nucleic acid isolated in the separation station and to incubate the contents of the receptacle, to which reagents for performing a nucleic acid amplification have been provided, for a period of time and under conditions sufficient to permit a target sequence of the target nucleic acid to be amplified, said incubator comprising: a housing having a side wall and including a receptacle access opening formed in the side wall for allowing the receptacle to be moved laterally into or out of said housing through said receptacle access opening in the side wall and a door configured for automated movement between a closed position in which said door cooperates with said housing to close said receptacle access opening and an open position in which said door is positioned such that said receptacle access opening permits a reaction receptacle to pass therethrough;a robotic pipettor positioned to access the contents of the receptacle;a detector for detecting a light signal emitted by the contents of a receptacle, wherein the separation station, the amplification incubator, the robotic pipettor and the detector are contained within a housing of the analyzer; anda computer controller in communication with the separation station, the amplification incubator, the robotic pipettor, and the detector and programmed to (i) effect cooperative operation of the separation station, the amplification incubator, the robotic pipettor, and the detector, (ii) control movement of a receptacle within the analyzer, (iii) control automated movement of the magnet moving structure between the first and second positions; (iv) control automated movement of said door between the closed position and the opened position, and (v) determine the presence of a target nucleic acid in the sample by: separating immobilized target nucleic acid from non-immobilized components of the sample at the separation station;forming a mixture with the robotic pipettor, the mixture comprising the separated target nucleic acid and all reagents necessary for performing a nucleic acid amplification;performing the nucleic acid amplification with the separated target nucleic acid to form an amplification product in the amplification incubator;detecting a signal associated with the amplification product with the detector; anddetermining the presence of the target nucleic acid based on the signal detected with the detector. 2. The automated analyzer of claim 1, wherein the magnetic element comprises a plurality of magnets carried by the magnet moving structure. 3. The automated analyzer of claim 1, comprising two or more magnetic elements held in an aligned arrangement on each of two sides of the magnet moving structure, and wherein the magnet moving structure pivots between the first and second positions. 4. The automated analyzer of claim 1, wherein the magnet moving structure is positioned on a processing deck, and the first position is a down position and the second position is an up position, relative to the processing deck. 5. The automated analyzer of claim 1, wherein the separation station includes a fluid aspirator mechanism configured to aspirate non-immobilized components of the sample after isolating the target nucleic acid. 6. The automated analyzer of claim 5, wherein the separation station further comprises a fluid dispense mechanism configured to dispense a wash buffer after aspirating non-immobilized components of the sample. 7. The automated analyzer of claim 1, wherein the separation station further comprises a mixing device configured to agitate the contents of a receptacle containing the solid support. 8. The automated analyzer of claim 7, wherein the separation station further comprises a separation station housing that includes a loading slot sized to receive a receptacle therethrough. 9. The automated analyzer of claim 8, wherein the separation station further comprises a carrier unit configured to receive and hold the receptacle within the separation station housing. 10. The automated analyzer of claim 9, wherein the separation station further comprises an orbital mixer assembly coupled to the carrier unit for orbitally mixing the contents of the receptacle when the receptacle is held by the carrier unit. 11. The automated analyzer of claim 1, wherein the amplification incubator comprises a carousel assembly configured to receive and hold a plurality of receptacles. 12. The automated analyzer of claim 1, further comprising a temperature ramping station that includes a heater or chiller for adjusting the temperature of the contents of a receptacle containing the separated target nucleic acid to the approximate temperature of the amplification incubator, wherein the temperature ramping station is located between the separation station and the amplification incubator. 13. The automated analyzer of claim 1 further comprising a holding station contained within the housing, the holding station being configured to hold a plurality of receptacles. 14. The automated analyzer of claim 1, further comprising a receptacle transport contained within the housing, the receptacle transport being configured to hold and move receptacles within the analyzer. 15. The automated analyzer of claim 14, wherein the receptacle transport is configured to engage and move receptacles between locations of the analyzer. 16. The automated analyzer of claim 15, wherein the receptacle transport has an extendible and retractable member for engaging and moving receptacles between locations of the analyzer. 17. The automated analyzer of claim 14, wherein the receptacle transport has a rotational path of travel. 18. The automated analyzer of claim 1 further comprising a hybridization incubator configured to provide a temperature-controlled environment to a mixture comprising the amplification product and a probe capable of detectably hybridizing to the amplification product. 19. The automated analyzer of claim 1 further comprising a detection station that includes the detector. 20. The automated analyzer of claim 1, wherein the detector comprises a photomultiplier tube for detecting the light signal emitted by the contents of a receptacle. 21. The automated analyzer of claim 1, wherein the detector comprises a diode for detecting the light signal emitted by the contents of a receptacle. 22. The automated analyzer of claim 1, wherein the receptacle is one of a plurality of receptacles formed as an integral array. 23. The automated analyzer of claim 22, wherein each of the plurality of receptacles comprises a cylindrical tube. 24. The automated analyzer of claim 1, wherein the separation station and the amplification incubator are positioned at different locations of a processing deck. 25. The automated analyzer of claim 1, wherein the analyzer contains all reagents necessary for performing a nucleic acid amplification. 26. The automated analyzer of claims 1, wherein the housing of the amplification incubator comprises a cylindrical portion defining the side wall and a top cover.
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