Methods of use of seed-origin endophyte populations
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-063/02
A01N-063/00
A01C-001/06
G01N-033/50
A01C-001/00
출원번호
US-0145687
(2016-05-03)
등록번호
US-9622485
(2017-04-18)
발명자
/ 주소
von Maltzahn, Geoffrey
Flavell, Richard Bailey
Toledo, Gerardo V.
Djonovic, Slavica
Marquez, Luis Miguel
Johnston, David Morris
Millet, Yves Alain
Lyford, Jeffrey
Naydich, Alexander
Sadowski, Craig
출원인 / 주소
Indigo Ag, Inc.
대리인 / 주소
Fenwick & West LLP
인용정보
피인용 횟수 :
10인용 특허 :
34
초록▼
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that includ
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.
대표청구항▼
1. A method of treating seeds of a wheat plant, comprising mechanically or manually inoculating a plurality of wheat seeds with an agricultural formulation comprising an agriculturally acceptable carrier and a purified bacterial population that comprises a seed bacterial endophyte of the genus Enter
1. A method of treating seeds of a wheat plant, comprising mechanically or manually inoculating a plurality of wheat seeds with an agricultural formulation comprising an agriculturally acceptable carrier and a purified bacterial population that comprises a seed bacterial endophyte of the genus Enterobacter that is heterologous to the seed, and has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO: 541, 595, 540, 550, 545 and 548, in an amount effective to increase tolerance to drought of the inoculated seed as compared to a reference seed sowed under the same conditions, wherein a plant, when germinated from the inoculated seed, has increased growth as compared to a plant germinated from the reference seed. 2. The method of claim 1, wherein the bacterial endophyte is obtainable from an interior seed compartment. 3. The method of claim 1, wherein the bacterial endophyte is obtainable from an exterior surface of a seed. 4. The method of claim 1, wherein the bacterial endophyte is obtainable from a different cultivar, variety or crop as compared to the seed. 5. The method of claim 1, wherein the bacterial endophyte colonizes the roots of a seedling germinated from the inoculated treated seed. 6. The method of claim 1, wherein the agricultural formulation further comprises a purified fungal population. 7. The method of claim 1, wherein the bacterial population includes two or more bacterial entophytes. 8. The method of claim 1, wherein the bacterial endophyte is obtained from rice seed, maize seed, wheat seed, or barley seed. 9. The method of claim 1, wherein the increased tolerance to drought occurs under conditions of biotic stress. 10. The method of claim 9, wherein the biotic stress is selected from the group consisting of nematode stress, insect herbivory stress, fungal pathogen stress, bacterial pathogen stress, and viral pathogen stress. 11. The method of claim 1, wherein the plant germinated from the inoculated seed has a larger amount of lateral roots and root-hairs as compared to the plant germinated from the reference seed. 12. The method of claim 1, wherein the plant germinated from the inoculated seed has increased shoot length as compared to the plant germinated from the reference seed. 13. The method of claim 1, wherein the plant germinated from the inoculated seed has increased seedling weight as compared to the plant germinated from the reference seed. 14. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 541. 15. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to SEQ ID NO: 541. 16. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 595. 17. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to SEQ ID NO: 595. 18. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 540. 19. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to SEQ ID NO: 540. 20. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 550. 21. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to SEQ ID NO: 550. 22. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 545. 23. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to SEQ ID NO: 545. 24. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 548. 25. The method of claim 1, wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to SEQ ID NO: 548.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (34)
Beaujot, Patrick M., Air seeder/fertilizer apparatus having metering means and distribution manifold with selectively openable ports.
Isawa, Tsuyoshi; Yasuda, Michiko; Shinozaki, Satoshi; Nakashita, Hideo; Kudo, Toshiaki, Bacteria and method for controlling plant disease using the same.
Henson, Joan M.; Sheehan, Kathy B.; Rodriguez, Russell J.; Redman, Regina S., Curvularia strains and their use to confer stress tolerance and/or growth enhancement in plants.
Leps Walter T. (3943 - 55 Street T6L 1C1 Edmonton ; Alberta CAX) Thompson Bradley G. (39 - 3115 - 119 Street T6J 5N5 Edmonton ; Alberta CAX), Method of adhering bacteria to seed and composition therefor.
Stritzker, Jochen Harald; Hill, Phil; Szalay, Aladar A.; Yu, Yong A., Methods and compositions for detection of microorganisms and cells and treatment of diseases and disorders.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
Imada,Takahiro; Hiruma,Naoya; Isawa,Tsuyoshi; Noda,Munehiro; Kurihara,Yohsuke; Kon,Madoka, Pest control method for grass family plants using endophytic bacteria, pest control material, and seed bound to the pest control material.
Hurley Richard H. (Flemington NJ) Funk ; Jr. Cyril R. (East Brunswick NJ), Plants having endophytio-fungus-enhanced performance and method of producing same.
Nielsen Sven-Erik (Trekanten 5 Hinnerup DKX) Sorensen Grete Morch (Trekanten 5 Hinnerup DKX DK-8382), Rhizobia transformants which symbiotically fixes nitrogen in non-legumes, a material for treating seeds of a non-legume.
Isawa,Tsuyoshi; Hiruma,Naoya; Imada,Takahiro; Noda,Munehiro; Kurihara,Yohsuke; Kon,Madoka, Rice containing endophytic bacteria and method of producing it.
Gao, Ai-Guo; Kolacz, Kathryn H.; MacRae, Ted C.; Miklos, John A.; Paradise, Mark S.; Perlak, Frederick J.; Dressel Toedebusch, Andrea S., Soybean plant and seed corresponding to transgenic event MON87701 and methods for detection thereof.
Denisov, Gennady A.; Arehart, Alan B.; Curtin, Michael D., System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms.
David M Tricoli ; Kim J. Carney ; Paul F. Russell ; Hector D. Quemada ; Russell J. McMaster ; John F. Reynolds ; Rosaline Z. Deng, Transgenic plants expressing DNA constructs containing a plurality of genes to impart virus resistance.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
Embree, Mallory, Methods, apparatuses, and systems for analyzing microorganism strains from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.