[특허]Thermus brockianus nucleic acid polymerases

Thermus brockianus nucleic acid polymerases 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-009/12 C12P-019/34
출원번호	US-0615041 (2012-09-13)
등록번호	US-9631182 (2017-04-25)
발명자 / 주소	Bolchakova, Elena Rozzelle, James
출원인 / 주소	Applied Biosystems, LLC
인용정보	피인용 횟수 : 0 인용 특허 : 78

초록

The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.

대표청구항 ▼

1. A mutant polymerase having at least 98% amino acid sequence identity to SEQ ID NO:9; wherein said polymerase comprises a G→D and/or a F→Y mutation corresponding to position 43 and/or position 665 of SEQ ID NO:9, respectively. 2. A mutant polymerase having at least 98% amino acid sequence identity to SEQ ID NO:9; wherein said polymerase comprises a G→D and/or a F→Y mutation corresponding to position 43 and/or position 665 of SEQ ID NO:9, respectively, made by a method comprising incubating a host cell under conditions sufficient for RNA transcription and translation comprising a nucleic acid encoding said mutant nucleic acid polymerase operably linked to a promoter. 3. The mutant polymerase of claim 2, wherein said nucleic acid comprises a nucleic acid sequence with at least 98% nucleic acid sequence identity to SEQ ID NO:1. 4. The mutant polymerase of claim 1, wherein said polymerase has DNA polymerase activity between 50,000 U/mg and 500,000 U/mg. 5. The mutant polymerase of claim 1, wherein said polymerase lacks 5′ to 3′ exonuclease activity. 6. The mutant polymerase of claim 1, wherein said polymerase lacks discrimination against dideoxynucleotide triphosphates (ddNTPs). 7. The mutant polymerase of claim 1, wherein said polymerase is thermostable. 8. The mutant polymerase of claim 1, wherein said polymerase comprises reverse transcriptase activity. 9. The mutant polymerase of claim 1, wherein said mutant polymerase has reduced bias against dideoxynucleotide triphosphates (ddNTP) incorporation compared to a corresponding wild type polymerase. 10. The mutant polymerase of claim 1, wherein said mutant polymerase has reduced 5′ to 3′ exonuclease activity compared to a corresponding wild type polymerase. 11. The mutant polymerase of claim 1, wherein said mutant polymerase has reduced 5′ to 3′ exonuclease activity and reduced bias against dideoxynucleotide triphosphates (ddNTP) incorporation compared to a corresponding wild type polymerase. 12. The mutant polymerase of claim 1, wherein said mutant polymerase lacks 5′ to 3′ exonuclease activity and lacks discrimination against dideoxynucleotide triphosphates (ddNTPs). 13. The mutant polymerase of claim 1, wherein said mutant polymerase is a mutant Thermus brockianus polymerase. 14. The mutant polymerase of claim 1, wherein said mutant polymerase comprises SEQ NO:11, 13, or 15.

이 특허에 인용된 특허 (78)

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연합인증

Thermus brockianus nucleic acid polymerases 원문보기

초록

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Thermus brockianus nucleic acid polymerases 원문보기

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