Nanolipoprotein particles comprising hydrogenases and related products, methods and systems
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07K-001/14
C12N-011/02
B82B-001/00
B82Y-005/00
C12P-003/00
출원번호
US-0352472
(2009-01-12)
등록번호
US-9688718
(2017-06-27)
발명자
/ 주소
Baker, Sarah E.
Chromy, Brett A.
Henderson, Paul
Hoeprich, Jr., Paul D.
출원인 / 주소
Lawrence Livermore National Security, LLC
대리인 / 주소
Steinfl + Bruno, LLP
인용정보
피인용 횟수 :
0인용 특허 :
11
초록
Provided herein are nanolipoprotein particles that comprise a membrane associated hydrogenase and related assemblies, devices, methods and systems.
대표청구항▼
1. A nanolipoprotein particle comprising a membrane associated hydrogenase,a membrane forming lipid, anda scaffold protein,wherein the membrane associated hydrogenase is heteromeric and multimeric; andwherein the nanolipoprotein particle further comprises a cell membrane fragment, the cell membrane
1. A nanolipoprotein particle comprising a membrane associated hydrogenase,a membrane forming lipid, anda scaffold protein,wherein the membrane associated hydrogenase is heteromeric and multimeric; andwherein the nanolipoprotein particle further comprises a cell membrane fragment, the cell membrane fragment comprising the membrane associated heteromeric and multimeric hydrogenase in a catalytically active form,the membrane forming lipid and the scaffold protein are at a mass ratio between 1:1 and 20:1 and the scaffold protein and the membrane associated heteromeric and multimeric hydrogenase are at a mass ratio between 1:0.025 and 1:1. 2. The nanolipoprotein particle of claim 1, wherein the membrane associated hydrogenase is a [Ni/Fe] hydrogenase or a [Fe/Fe] hydrogenase. 3. The nanolipoprotein particle of claim 1, wherein the membrane associated hydrogenase is a [Ni/Fe] hydrogenase from Allochromatium vinosum, Methanosarcina barkeri, Escherichia coli, and Rhodospirillum rubrum Desulfomicrobium baculatum and Ralstonia species. 4. The nanolipoprotein particle of claim 1, wherein the membrane associated hydrogenase is a [Ni/Fe] hydrogenase from Pyrococcus Furiosus. 5. A biocatalyst assembly comprising the nanolipoprotein particle of claim 1, wherein the nanolipoprotein particle is immobilized to a support. 6. The biocatalyst assembly of claim 5, wherein the support is a solid support. 7. The biocatalyst assembly of claim 5, wherein the support is an electrically conductive support. 8. A method to perform a chemical reaction catalyzed by a membrane associated hydrogenase, the method comprising: providing reagents for performing said chemical reaction,providing the nanolipoprotein particle of claim 1, wherein the membrane associated hydrogenase is the membrane associated hydrogenase catalyzing the chemical reaction; andcontacting the reagents with the nanolipoprotein particle for a time and under conditions to allow the reaction to occur, thus performing said chemical reaction. 9. The method of claim 8 wherein the nanolipoprotein particle is immobilized on a solid support. 10. The method of claim 8, wherein the chemical reaction is hydrogen production. 11. The method of claim 10 wherein the hydrogen production is performed as a solution phase reaction. 12. The method of claim 10, wherein the conditions include a temperature between from about 25 degrees C. and about 95 degrees C. 13. The method of claim 8, wherein the membrane associated hydrogenase is a [Ni/Fe] hydrogenase. 14. The method of claim 8, wherein the chemical reaction is oxidation of molecular hydrogen. 15. A system to perform a chemical reaction catalyzed by a membrane associated hydrogenase, the system comprising: the nanolipoprotein particle of claim 1, wherein the membrane associated hydrogenase is the membrane associated hydrogenase catalyzing the chemical reaction; andreagents for performing the chemical reaction,wherein the nanolipoprotein particle and the reagents can be contacted for a time and under conditions to allow the chemical reaction catalyzed by said membrane associated hydrogenase to occur. 16. The system of claim 15, wherein the membrane associated hydrogenase is immobilized to a support. 17. The system of claim 15, wherein the membrane associated hydrogenase is a [Ni/Fe] hydrogenase from Allochromatium vinosum, Methanosarcina barkeri, Escherichia coli, Rhodospirillum rubrum, Desulfomicrobium baculatum and Ralstonia species. 18. The system of claim 15, wherein the membrane associated hydrogenase is a [Ni/Fe] hydrogenase from Pyrococcus Furiosus. 19. A device for performing a chemical reaction catalyzed by a membrane associated hydrogenase, the device comprising: a substrate compartment, a hydrogenase compartment, and a product compartment, with the substrate compartment in communication with the product compartment through the hydrogenase compartment,whereinthe hydrogenase compartment comprises the biocatalyst assembly of claim 5; andthe substrate compartment is configured to comprise a substrate for the chemical reaction catalyzed by the membrane associated hydrogenase and to allow contact between said substrate and said membrane associated hydrogenase. 20. The device of claim 19, further comprising a product compartment in communication with the hydrogenase compartment, the product compartment configured to comprise hydrogen produced following the reaction of a substrate with the membrane associated hydrogenase. 21. A nanolipoprotein particle comprising a membrane associated hydrogenase,a membrane forming lipid, anda scaffold protein,wherein the membrane associated hydrogenase is heteromeric and multimeric in a catalytically active form; andwherein the nanolipoprotein particle is obtained from a cell membrane fragment, the cell membrane fragment comprising the membrane associated heteromeric and multimeric hydrogenase,the membrane forming lipid and the scaffold protein are at a mass ratio between 1:1 and 20:1 and the scaffold protein and the membrane associated heteromeric and multimeric hydrogenase are at a mass ratio between 1:0.025 and 1:1. 22. The nanolipoprotein particle of claim 1, wherein the membrane forming lipid is dimyristoylphosphatidylcholine (DMPC), the scaffold protein is Apo E422K, and the DMPC, Apo E422K and the membrane associated heteromeric and multimeric hydrogenase have a mass ratio of about 4:1:0.05. 23. The nanolipoprotein particle of claim 21, wherein the membrane forming lipid is dimyristoylphosphatidylcholine (DMPC), the scaffold protein is Apo E422K, and the DMPC, Apo E422K and the membrane associated heteromeric and multimeric hydrogenase have a mass ratio of about 4:1:0.05.
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이 특허에 인용된 특허 (11)
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