The present invention relates to methods for determining the concentration of a free beta-lactam antibiotic in a biological sample. In particular, the present invention relates to methods for measuring a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at le
The present invention relates to methods for determining the concentration of a free beta-lactam antibiotic in a biological sample. In particular, the present invention relates to methods for measuring a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase, a functional fragment or variant thereof; (b) providing at least one biological sample; (c) contacting said at least one class C beta-lactamase with said at least one biological sample; and (d) determining the concentration of said free beta-lactam antibiotic in said at least one biological sample.
대표청구항▼
1. A method for determining concentration of a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase comprising an amino acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10 and SEQ ID NO:1
1. A method for determining concentration of a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase comprising an amino acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10 and SEQ ID NO:12;(b) providing at least one biological sample;(c) contacting said at least one class C beta-lactamase with said at least one biological sample; and(d) determining said concentration of said free beta-lactam antibiotic in said at least one biological sample. 2. A method for determining concentration of a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase comprising an amino acid sequence of SEQ ID NO:8 or SEQ ID NO:12;(b) providing at least one biological sample;(c) contacting said at least one class C beta-lactamase with said at least one biological sample; and(d) determining said concentration of said free beta-lactam antibiotic in said at least one biological sample. 3. The method according to claim 1, wherein said biological sample is selected from the group comprising serum, blood, urine, interstitial fluid, saliva, tears, exudates, fluid collected from a deep tissue, fluid collected from a subcutaneous tissue. 4. The method of claim 3, wherein said biological sample is serum. 5. The method according to claim 1, wherein said biological sample is obtained from a subject receiving an antibiotherapy. 6. The method according to claim 1, wherein said biological sample is obtained from a subject being treated for an infection caused by Gram positive and/or Gram negative micro-organisms. 7. The method according to claim 2, wherein said biological sample is selected from the group comprising serum, blood, urine, interstitial fluid, saliva, tears, exudates, fluid collected from a deep tissue, fluid collected from a subcutaneous tissue, preferably said biological sample is serum. 8. The method of claim 7, wherein said biological sample is serum. 9. The method according to claim 2, wherein said biological sample is obtained from a subject receiving an antibiotherapy. 10. The method according to claim 2, wherein said biological sample is obtained from a subject being treated for an infection caused by Gram positive and/or Gram negative micro-organisms.
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