초록 ▼

In one aspect, the invention relates to an isolated polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 71 in another aspect, the invention relates to an immunogenic composition including an isolated non-lipidated, non-pyruvylated ORF2086 polypeptide from Neiss

In one aspect, the invention relates to an isolated polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 71 in another aspect, the invention relates to an immunogenic composition including an isolated non-lipidated, non-pyruvylated ORF2086 polypeptide from Neisseria meningitidis serogroup B, and at least one conjugated capsular saccharide from a meningococcal serogroup.

대표청구항 ▼

1. An isolated non-lipidated and non-pyruvylated polypeptide comprising an amino acid sequence set forth as SEQ ID NO: 68, wherein an N-terminal cysteine at position 1 is deleted, as compared to SEQ ID NO: 15. 2. An isolated non-lipidated and non-pyruvylated polypeptide comprising an amino acid sequ

1. An isolated non-lipidated and non-pyruvylated polypeptide comprising an amino acid sequence set forth as SEQ ID NO: 68, wherein an N-terminal cysteine at position 1 is deleted, as compared to SEQ ID NO: 15. 2. An isolated non-lipidated and non-pyruvylated polypeptide comprising an amino acid sequence set forth as SEQ ID NO: 68, wherein an N-terminal cysteine at position 1 is substituted, as compared to SEQ ID NO: 15. 3. The polypeptide according to claim 1, wherein the polypeptide is immunogenic. 4. The polypeptide according to claim 1, wherein the polypeptide does not exhibit a mass shift of about +70 Da compared to the corresponding non-lipidated polypeptide as measured by mass spectrometry. 5. The isolated polypeptide according to claim 1, wherein the amino acid sequence consists of the sequence set forth in SEQ ID NO: 68. 6. The isolated polypeptide according to claim 5, wherein the polypeptide is encoded by the nucleic acid sequence SEQ ID NO: 69. 7. The isolated polypeptide according to claim 1 wherein the amino acid sequence consists of the sequence set forth as SEQ ID NO: 64. 8. The isolated polypeptide according to claim 7, wherein the polypeptide is encoded by the nucleic acid sequence SEQ ID NO: 63. 9. An immunogenic composition comprising the polypeptide as in any of claims 1, 3, 4, 5, 6, 7, and 6. 10. The composition according to claim 9, further comprising an adjuvant.