A microfluidic apparatus mounted on a rotation driving unit for inducing a fluid to flow due to a centrifugal force includes: a target chamber that houses a first fluid; a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radius d
A microfluidic apparatus mounted on a rotation driving unit for inducing a fluid to flow due to a centrifugal force includes: a target chamber that houses a first fluid; a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radius direction than the target chamber, the first chamber being connected to the target chamber by a first channel; a first valve that prevents a flow of the second fluid through the first channel; and a second chamber disposed closer to the rotation center in the radius direction than the target chamber and connected to the target chamber by a second channel, wherein the first fluid is transported to the second chamber by supplying the second fluid to the target chamber by the centrifugal force.
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1. A microfluidic apparatus for inducing a fluid to flow due to a centrifugal force, the apparatus comprising: a target chamber that houses a first fluid;a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radial direction than th
1. A microfluidic apparatus for inducing a fluid to flow due to a centrifugal force, the apparatus comprising: a target chamber that houses a first fluid;a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radial direction than the target chamber, the first chamber being connected to the target chamber by a first channel;a first valve that controls a flow of the second fluid through the first channel; anda second chamber disposed closer to the rotation center in radial radius direction than the target chamber and connected to the target chamber by a second channel, whereinthe first channel is connected to the target chamber at a first location and the second channel is connected to the target chamber at a second location other than the first location,in an initial state, the second fluid in the first chamber has a first fluid level which is higher than a second fluid level of the first fluid in the target chamber from a perspective of the rotation center of the microfluidic device, andwhen the second fluid in the first chamber is supplied to the target chamber from the first chamber by the centrifugal force, the first fluid is pushed by the second fluid and is transported from the target chamber to the second chamber until the first and second fluids in the first and second chambers, the target chamber, and the first and second channels reach an equilibrium state, wherein fluid levels of fluids in the first chamber and the second chamber are at a third fluid level lower than the first fluid level. 2. The microfluidic apparatus of claim 1, further comprising a second valve that controls the flow of the first fluid through the second channel. 3. The microfluidic apparatus of claim 1, wherein the first fluid and the second fluid are immiscible. 4. The microfluidic apparatus of claim 1, wherein the first channel is filled with a gas. 5. The microfluidic apparatus of claim 1, further comprising a first processing unit that processes a sample, supplies the first fluid including a target material to the target chamber, and discharges the second fluid, wherein the second fluid comprises a waste fluid that is separated from the sample. 6. The microfluidic apparatus of claim 5, further comprising a second processing unit disposed in an outer portion of the microfluidic apparatus and that receives the first fluid from the second chamber. 7. A microfluidic apparatus for inducing a fluid to flow due to a centrifugal force, the apparatus comprising: a first processing unit for separating a target cell from a sample;a target chamber disposed in an outer portion of the microfluidic apparatus and that receives a first fluid including the target cell from the first processing unit;a first chamber that houses a second fluid, is disposed closer to a rotation center of the microfluidic apparatus in a radial direction than the target chamber, and is connected to the target chamber by a first channel;a second chamber disposed closer to the rotation center than the target chamber and connected to the target chamber by a second channel; anda second processing unit disposed in the outer portion of the microfluidic apparatus and connected to the second chamber, whereinthe first channel is connected to the target chamber at a first location and the second channel is connected to the target chamber at a second location other than the first location, andin an initial state, the second fluid in the first chamber has a first fluid level which is higher than a second fluid level of the first fluid in the target chamber from a perspective of the rotation center of the microfluidic device, andwhen the second fluid in the first chamber is supplied to the target chamber from the first chamber by the centrifugal force, the first fluid is pushed by the second fluid and is transported from the target chamber to the second chamber until fluid levels of fluids in the first chamber and the second chamber become lower than the first fluid. 8. The microfluidic apparatus of claim 7, further comprising a first valve that controls a flow of the second fluid through the first channel. 9. The microfluidic apparatus of claim 7, further comprising a second valve that controls a flow of the first fluid through the second channel. 10. The microfluidic apparatus of claim 7, wherein the first fluid and the second fluid are immiscible. 11. The microfluidic apparatus of claim 7, wherein the first channel is filled with a gas. 12. The microfluidic apparatus of claim 7, wherein the first processing unit comprises a sample chamber in which a complex in which fine beads are bound to the target cell in the sample is formed; and a separation chamber wherein the complex is separated from the sample using a density gradient medium with a density lower than that of the complex and higher than that of other materials in the sample, wherein the first fluid including the complex is provided to the target chamber. 13. The microfluidic apparatus of claim 12, wherein a waste chamber is connected to the sample chamber, and a waste fluid comprising an upper material layer above the target cell in the sample chamber is discharged from the sample chamber to the waste chamber. 14. The microfluidic apparatus of claim 7, wherein the second processing unit receives the first fluid from the second chamber. 15. The microfluidic apparatus of claim 7, wherein the target cell is a circulating tumor cell, a cancer stem cell, or a cancer cell.
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이 특허에 인용된 특허 (1)
Kellogg Gregory ; Kieffer-Higgins Stephen G. ; Carvalho Bruce L. ; Davis Gene A. ; Willis John P. ; Minior Ted ; Chapman Laura L. ; Kob Mikayla ; Oeltjen Sarah D. ; Ommert Shari ; Mian Alec, Devices and methods for using centripetal acceleration to drive fluid movement on a microfluidics system.
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