System and methods for making and processing emulsions
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
B01F-005/06
C12M-001/34
C12M-003/00
B01F-003/08
B01F-005/04
B01F-013/00
B04B-005/04
B04B-007/02
B01L-003/00
출원번호
US-0696642
(2015-04-27)
등록번호
US-9776146
(2017-10-03)
발명자
/ 주소
Schultz, Jonathan
Nobile, John
Reed, Brian
Thwar, Prasanna Krishnan
Roswech, Todd
출원인 / 주소
Life Technologies Corporation
인용정보
피인용 횟수 :
0인용 특허 :
13
초록▼
An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generatio
An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
대표청구항▼
1. An emulsion generating device comprising: a first gasket including a first set of channels;a second gasket including a second set of channels complementary to the first set of channels, a carrier fluid inlet and an emulsion outlet fluidically coupled to the second set of channels; anda membrane d
1. An emulsion generating device comprising: a first gasket including a first set of channels;a second gasket including a second set of channels complementary to the first set of channels, a carrier fluid inlet and an emulsion outlet fluidically coupled to the second set of channels; anda membrane disposed between the first and second gaskets,wherein the first set of channels and second set of channels are complementary and align to permit fluid flow from a first channel of the first set of channels to a first channel of the second set of channels, from the first channel of the second set of channels to a second channel of the first set of channels, and from the second channel of the first set of channels to a second channel of the second set of channels, andwherein fluid is to pass through the membrane at least three times when traversing the first and second sets of channels between the carrier fluid inlet and the emulsion outlet. 2. The emulsion generating device of claim 1, wherein the first and second sets of channels are concurrently complementary. 3. The emulsion generating device of claim 1, wherein the first and second sets of channels are countercurrently complementary. 4. The emulsion generating device of claim 1, wherein the membrane comprises laser etched holes. 5. The emulsion generating device of claim 1, wherein a depth of at least one of the first and second sets of channels is not greater than 500 micrometers. 6. The emulsion generating device of claim 1, further comprising a housing, the first and second channel gasket and the membrane disposed within the housing. 7. The emulsion generating device of claim 6, wherein the housing defines a carrier fluid inlet port in fluidic communication with the carrier fluid inlet of the second gasket and defines an emulsion outlet port in fluidic communication with the emulsion outlet of the second gasket. 8. The emulsion generating device of claim 6, wherein the housing defines a sample tube receptacle. 9. The emulsion generating device of claim 8, wherein the housing further defines a displacement fluid inlet port in fluidic communication with the sample tube receptacle. 10. The emulsion generating device of claim 8, further comprising a sample fluid tube. 11. An emulsion generating system comprising: an emulsion generating device comprising: a housing defining a sample inlet port, a carrier fluid inlet port, an emulsion outlet port, and a displacement fluid inlet port;a first gasket including a first set of channels;a second gasket including a second set of channels complementary to the first set of channels, a carrier fluid inlet and the emulsion outlet port fluidically coupled to the second set of channels; anda membrane disposed between the first and second gaskets,wherein the first set of channels includes at least two channels each having at least two portions and the second set of channels includes at least three channels each having at least two portions, andwherein the first gasket and the second gasket are aligned such that each channel in the first set of channels has one portion overlapping one of the channels in the second set of channels and another portion overlapping a different one of the channels in the second set of channels; anda sample tube disposed on the sample inlet port and in fluidic communication with the displacement fluid inlet port. 12. The emulsion generating system of claim 11, wherein the carrier fluid inlet port and the emulsion outlet port are disposed on a lower surface of the housing. 13. The emulsion generating system of claim 11, wherein the sample inlet port is disposed on an upper surface of the housing. 14. The emulsion generating system of claim 13, wherein the displacement fluid port is disposed on the upper surface of the housing. 15. The emulsion generating system of claim 13, wherein the displacement fluid port is disposed on a lower surface of the housing, a passageway defined between the displacement fluid port and the sample tube through the first and second channel gaskets and the membrane. 16. The emulsion generating system of claim 11, wherein the first and second sets of channels are concurrently complementary. 17. The emulsion generating system of claim 11, wherein the first and second sets of channels are countercurrently complementary. 18. A method of generating an emulsion, the method comprising: applying an aqueous sample solution to a sample tube of an emulsion generating system comprising: an emulsion generating device comprising: a housing defining a sample inlet port, a carrier fluid inlet port, an emulsion outlet port, and a displacement fluid inlet port;a first gasket including a first set of channels;a second gasket including a second set of channels complementary to the first set of channels, a carrier fluid inlet and the emulsion outlet port fluidically coupled to the second set of channels; anda membrane disposed between the first and second gaskets,wherein the first set of channels includes at least two channels each having at least two portions and the second set of channels includes at least three channels each having at least two portions, andwherein the first gasket and the second gasket are aligned such that each channel in the first set of channels has one portion overlapping one of the channels in the second set of channels and another portion overlapping a different one of the channels in the second set of channels; anda sample tube disposed on the sample inlet port and in fluidic communication with the displacement fluid inlet port,applying a displacement fluid through the displacement fluid inlet port;applying a carrier fluid through the carrier fluid inlet port; andcollecting an emulsion through the emulsion outlet port. 19. The method of claim 18, wherein the displacement fluid and the carrier fluid comprise an oil. 20. The method of claim 18, wherein the displacement fluid displaces the aqueous sample fluid from the sample tube.
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