Preparation and use of plant embryo explants for transformation
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-015/82
A01H-004/00
C12N-005/04
G01N-033/00
C12N-009/10
출원번호
US-0642671
(2015-03-09)
등록번호
US-9790512
(2017-10-17)
발명자
/ 주소
Calabotta, Beth Jo
Deppermann, Kevin Lee
Dersch, Erik D.
Heise, Jerald D.
Koestel, Angela Ranae
Ludwig, Cindy L.
Martinell, Brian J.
Williams, Edward
출원인 / 주소
Monsanto Technology LLC
대리인 / 주소
Dentons US LLP
인용정보
피인용 횟수 :
0인용 특허 :
65
초록▼
The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transform
The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
대표청구항▼
1. A method for obtaining a transformable plant tissue comprising: (a) obtaining a plant seed; and(b) preparing an explant, wherein the explant comprises meristematic tissue, from the plant seed under conditions wherein the explant does not germinate and remains viable and competent for genetic tran
1. A method for obtaining a transformable plant tissue comprising: (a) obtaining a plant seed; and(b) preparing an explant, wherein the explant comprises meristematic tissue, from the plant seed under conditions wherein the explant does not germinate and remains viable and competent for genetic transformation; and(c) transforming at least a first cell of the explant with a selected DNAand wherein the plant seed from which the explant is prepared comprises an internal moisture content of from about 4% to about 16%. 2. The method of claim 1, further comprising the step of: (d) regenerating a transgenic plant from said cell, wherein the plant is stably transformed with the selected DNA. 3. The method of claim 2, further defined as comprising storing the explant prior to step (d) under conditions wherein the explant does not germinate. 4. The method of claim 1, further defined as comprising dehydrating said seed and/or explant prior to or after preparing the explant. 5. The method of claim 2, further defined as comprising increasing the moisture content of the explant prior to or concurrently with step (d). 6. The method of claim 1, wherein preparing the explant is carried out in the absence of a liquid. 7. The method of claim 1, wherein the explant comprises an internal moisture content of from about 3% to about 25%. 8. The method of claim 1, further defined as comprising priming the plant seed and/or the explant prior to or concurrently with step (c). 9. The method of claim 8, wherein priming the explant comprises contacting the explant with an aqueous solution. 10. The method of claim 9, wherein the aqueous solution comprises a disinfectant. 11. The method of claim 1, wherein the plant seed is a soybean, canola, corn, cotton, onion, pepper, tomato, cucurbit, rice, or wheat seed. 12. The method of claim 1, wherein step (c) is carried out by bacterially-mediated transformation or microprojectile bombardment. 13. The method of claim 2, further defined as comprising disinfecting the plant seed and/or the explant prior to step (d). 14. The method of claim 13, wherein disinfecting comprises application of a disinfectant selected from the group consisting of bleach, alcohol, ozone, chlorine gas, ultraviolet light, temperatures of −20° C. or lower, and exposure to a temperature higher than 50° C. 15. The method of claim 14, wherein disinfecting comprises application of a nystatin and/or thiabendazole. 16. The method of claim 2, wherein the method is carried out without manipulating the development of the explant with a plant growth regulator. 17. The method of claim 2, wherein step (d) comprises culturing the explant under lighting conditions that allow for normal plastid development. 18. A method for producing a transformable plant tissue comprising: (a) obtaining an explant from a plant seed, wherein the explant comprises meristematic tissue and wherein the plant seed from which the explant is obtained comprises an internal moisture content of from about 4% to about 16%; and(b) dehydrating the plant seed and/or explant prior to, concurrently with or after said step (a) to obtain an explant that does not germinate and remains viable and competent for genetic transformation. 19. The method of claim 18, further comprising the step of: (c) hydrating said explant; and(d) regenerating a plant from the explant. 20. The method of claim 19, further comprising the step of: (e) transforming at least a first cell of the explant with a selected DNA prior to the step of regenerating the plant from the explant, wherein the plant is stably transformed with the selected DNA. 21. The method of claim 20, wherein hydrating said explant is performed under dark conditions. 22. The method of claim 1, wherein the explant is prepared without hydrating the plant seed prior to step (b). 23. The method of claim 2, wherein the transgenic plant is regenerated without producing a callus tissue culture. 24. The method of claim 7, wherein the explant comprises an internal moisture content of from about 4% to about 16%. 25. The method of claim 18, wherein the explant is prepared without hydrating the plant seed prior to the obtaining step (a). 26. The method of claim 18, wherein the explant comprises an internal moisture content of from about 3% to about 25%. 27. The method of claim 19, wherein the plant is regenerated without producing a callus tissue culture. 28. The method of claim 27, wherein the explant comprises an internal moisture content of from about 4% to about 16%.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (65)
Jiao,Gai Li; Liu,Jian Wei, -mediated transformation of cotton with novel explants.
Hanson William (San Francisco CA) Morgan Alison (Fremont CA) Robinson Karol E. P. (Moraga CA) Ruby Karen L. (Berkeley CA), Genetically transformed pea plants and methods for their production.
Engler Dean E. (El Cerrito CA) Guri Assaf Z. (Cherry Hill NJ) Lauritis James A. (Maple Shade NJ) Schloemer Lucille M. P. (El Cerrito CA), Genetically transformed pepper plants and methods for their production.
Giguere R. James (Kansas City MO), Method and apparatus for degerminating a grain kernel by impelling the kernels along a guide vane into an impact surface.
Martinell, Brian J.; Calabotta, Beth Jo; Heinzen, Richard J.; Klemm, Richard F.; McCabe, Dennis E.; Roberts, Gail A.; Smith, Lori Ann, Method and apparatus for preparation of genetically transformable plant tissue.
Martinell, Brian J.; Calabotta, Beth Jo; Heinzen, Richard J.; Klemm, Richard F.; McCabe, Dennis E.; Roberts, Gail A.; Smith, Lori Ann, Method and apparatus for preparation of genetically transformable plant tissue.
Adams Thomas R. (No. Stonington CT) Chambers Sheryl A. (Groton CT) Daines Richard J. (Ledyard CT) Gordon-Kamm William J. (Stonington CT) Kausch Albert P. (Stonington CT) Lemaux Peggy G. (Mystic CT) M, Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof.
Christou Paul (Madison WI) McCabe Dennis (Middleton WI) Swain William F. (Madison WI) Barton Kenneth A. (Middleton WI), Particle-mediated transformation of soybean plants and lines.
McKersie Bryan D. (Kenilworth CAX) Senaratna Tissa (Guelph CAX) Bowley Steve (Guelph CAX) Bewley J. Derek (Guelph CAX) Brown Daniel C. W. (Nepean CAX), Process to induce desiccation tolerance in somatic embryos.
Brian J. Martinell ; Lori S. Julson ; Carol A. Emler ; Yong Huang ; Dennis E. McCabe ; Edward J. Williams, Soybean agrobacterium transformation method.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.