최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0233835 (2016-08-10) |
등록번호 | US-9839894 (2017-12-12) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 2 인용 특허 : 429 |
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Fu
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
1. A polynucleotide cDNA library, comprising at least 30,000 non-identical polynucleotides that collectively encode cDNA sequences corresponding to at least 750 genes or gene fragments, wherein each of the at least 750 genes or gene fragments comprises an intron, wherein the at least 30,000 non-iden
1. A polynucleotide cDNA library, comprising at least 30,000 non-identical polynucleotides that collectively encode cDNA sequences corresponding to at least 750 genes or gene fragments, wherein each of the at least 750 genes or gene fragments comprises an intron, wherein the at least 30,000 non-identical polynucleotides comprise a plurality of subsets of non-identical polynucleotides, wherein each of the subsets of non-identical polynucleotides collectively encodes for one of the cDNA sequences and comprises polynucleotides varying within a range of lengths, and wherein each of the at least 30,000 non-identical polynucleotides comprises a first overlap region which is complementary to a second overlap region of another non-identical polynucleotide of the at least 30,000 non-identical polynucleotides. 2. The polynucleotide cDNA library of claim 1, wherein each non-identical polynucleotide is at least 50 bases in length. 3. The polynucleotide cDNA library of claim 1, wherein each non-identical polynucleotide is at least 100 bases in length. 4. The polynucleotide cDNA library of claim 1, wherein each non-identical polynucleotide is 30 to 500 bases in length. 5. The polynucleotide cDNA library of claim 1, wherein the library comprises at least 300,000 non-identical polynucleotides. 6. The polynucleotide cDNA library of claim 5, wherein the at least 300,000 non-identical polynucleotides collectively encode cDNA sequences corresponding to at least 6000 genes or gene fragments. 7. The polynucleotide cDNA library of claim 1, wherein the at least 30,000 non-identical polynucleotides are attached to a structure. 8. The polynucleotide cDNA library of claim 7, wherein the structure is a solid support. 9. The polynucleotide cDNA library of claim 1, wherein each of the cDNA sequences is at least 0.5 kb in length. 10. The polynucleotide cDNA library of claim 1, wherein each of the polynucleotides varying within a range of lengths are of a different length. 11. The polynucleotide cDNA library of claim 1, wherein the polynucleotides varying within a range of lengths are of at least 10 different lengths. 12. The polynucleotide cDNA library of claim 1, wherein the polynucleotides varying within a range of lengths are of at least 30 different lengths. 13. The polynucleotide cDNA library of claim 1, wherein the first overlap region comprises a GC content of 35% to 65%. 14. The polynucleotide cDNA library of claim 1, wherein the first overlap region comprises 10 to 100 bases in length. 15. The polynucleotide cDNA library of claim 1, wherein each non-identical polynucleotide is at least 25 bases in length. 16. The polynucleotide cDNA library of claim 1, wherein each of the cDNA sequences is at least 3 kb in length. 17. The polynucleotide cDNA library of claim 1, wherein the library comprises at least 100,000 non-identical polynucleotides.
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