Rapid detection of the “high virulent” ST-17 clone of group B Streptococcus
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07K-016/00
G01N-033/53
G01N-033/569
C12Q-001/68
C07K-016/12
출원번호
US-0291120
(2016-10-12)
등록번호
US-9874560
(2018-01-23)
우선권정보
EP-05292747 (2005-12-20)
발명자
/ 주소
Poyart, Claire
Lamy, Marie-Cecile
Dramsi, Shaynoor
Sauvage, Elisabeth
Glaser, Philippe
Trieu-Cuot, Patrick
출원인 / 주소
INSTITUT PASTEUR
대리인 / 주소
Arrigo, Lee & Guttman LLP
인용정보
피인용 횟수 :
0인용 특허 :
4
초록▼
The present invention relates to polynucleotides enabling the rapid, simple and specific detection of Group B Streptococcus highly-virulent ST-17 clones. The present invention also relates to the polypeptides encoded by said polynucleotides, as well as to antibodies directed or raised against said p
The present invention relates to polynucleotides enabling the rapid, simple and specific detection of Group B Streptococcus highly-virulent ST-17 clones. The present invention also relates to the polypeptides encoded by said polynucleotides, as well as to antibodies directed or raised against said polypeptides. The present invention also relates to kits and methods for the specific detection of Group B Streptococcus highly-virulent ST-17 clones, using the polynucleotides, the polypeptides or the antibodies according to the invention.
대표청구항▼
1. An isolated monoclonal antibody for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone, wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or t
1. An isolated monoclonal antibody for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone, wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or the S11b amino acid sequence of SEQ ID NO:16, but does not bind specifically to a polypeptide that does not have the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or the S11b amino acid sequence of SEQ ID NO:16. 2. The isolated monoclonal antibody of claim 1, wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6. 3. The isolated monoclonal antibody of claim 1, wherein the monoclonal antibody binds specifically to a polypeptide having the S11a amino acid sequence of SEQ ID NO:14. 4. The isolated monoclonal antibody of claim 1, wherein the monoclonal antibody binds specifically to a polypeptide having the S11b amino acid sequence of SEQ ID NO:16. 5. A kit for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone comprising at least one positive or negative control and a monoclonal antibody that binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or the S11b amino acid sequence of SEQ ID NO:16, but does not bind specifically to a polypeptide that does not have the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or the S11b amino acid sequence of SEQ ID NO:16. 6. The kit of claim 5, wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6. 7. The kit of claim 5, wherein the monoclonal antibody binds specifically to a polypeptide having the S11a amino acid sequence of SEQ ID NO:14. 8. The kit of claim 5, wherein the monoclonal antibody binds specifically to a polypeptide having the S11b amino acid sequence of SEQ ID NO:16. 9. A method for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone in a biological sample comprising: providing a biological sample;contacting the biological sample with an antibody that binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or the S11b amino acid sequence of SEQ ID NO:16, but does not bind specifically to a polypeptide that does not have the S10 amino acid sequence of SEQ ID NO:6, the S11a amino acid sequence of SEQ ID NO:14, or the S11b amino acid sequence of SEQ ID NO:16; anddetecting the presence or absence of an antibody/antigen complex. 10. The method of claim 9, wherein the antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:6. 11. The method of claim 9, wherein the antibody binds specifically to a polypeptide having the S11a amino acid sequence of SEQ ID NO:14. 12. The method of claim 9, wherein the antibody binds specifically to a polypeptide having the S11b amino acid sequence of SEQ ID NO:16. 13. The method of claim 9, wherein the biological sample is from a pregnant woman. 14. The method of claim 9, wherein the biological sample is from a fetus. 15. The method of claim 9, wherein the biological sample is from a neonate. 16. The method of claim 9, wherein the biological sample is from a mammal. 17. The method of claim 9, wherein the biological sample is from a human. 18. The method of claim 10, wherein the antibody is monoclonal. 19. The method of claim 11, wherein the antibody is monoclonal. 20. The method of claim 12, wherein the antibody is monoclonal.
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이 특허에 인용된 특허 (4)
Mierendorf Robert C. (Madison WI) Morris Barbara B. (Madison WI), Method and kit for making CDNA library.
Poyart, Claire; Lamy, Marie-Cecile; Dramsi, Shaynoor; Couve, Elisabeth; Glaser, Philippe; Trieu-Cuot, Patrick, Rapid detection of the “high-virulent” ST-17 clone of group B Streptococcus .
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