최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
---|---|
국제특허분류(IPC7판) |
|
출원번호 | US-0188660 (2014-02-24) |
등록번호 | US-9926521 (2018-03-27) |
발명자 / 주소 |
|
출원인 / 주소 |
|
대리인 / 주소 |
|
인용정보 | 피인용 횟수 : 0 인용 특허 : 297 |
The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles,
The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.
1. A microfluidic system for cell manipulation comprising: (a) an input mechanism for introducing a fluid sample containing a plurality of eukaryotic cells;(b) a microfluidic passage in fluid communication with said input mechanism;(c) a flow-based positioning mechanism in fluid communication with s
1. A microfluidic system for cell manipulation comprising: (a) an input mechanism for introducing a fluid sample containing a plurality of eukaryotic cells;(b) a microfluidic passage in fluid communication with said input mechanism;(c) a flow-based positioning mechanism in fluid communication with said microfluidic passage, said positioning mechanism for positioning at least one cell of the plurality of cells in a retention mechanism while contained in said fluid sample; wherein the retention mechanism is for retaining one individual cell upon being positioned by said positioning mechanism,wherein said retention mechanism is configured to retain a single cell and comprises a physical barrier that restricts longitudinal cell movement along the direction of fluid flow and restricts orthogonal cell movement, andwherein the flow-based positioning mechanism comprises a dividing flow site formed by a junction of the microfluidic passage, a first outlet channel, and a second outlet channel, such that when the fluid sample containing the plurality of eukaryotic cells is introduced, said one individual cell is delivered to and retained by the retention mechanism, at least some unretained cells flow through the first outlet channel, and at least some unretained cells flow through the second outlet channel; and,(d) a release mechanism for releasing said cell from said retention mechanism. 2. The microfluidic system of claim 1 wherein the flow-based positioning mechanism comprises a dividing flow site formed by a junction of the microfluidic passage, a first outlet channel, and a second outlet channel, such that when the fluid sample containing the plurality of eukaryotic cells is introduced, said one individual cell is delivered to and retained by the retention mechanism, at least some unretained cells flow through the first outlet channel, and at least some unretained cells flow through the second outlet channel, wherein said individual cell, when retained by said retention mechanism, blocks fluid flow through the retention mechanism so that the retention mechanism preferentially retains only one cell. 3. The microfluidic system of claim 1 wherein said release mechanism is for terminating the flow-based positioning mechanism and releasing said cell from said retention mechanism. 4. The microfluidic system of claim 1 wherein said cell, when retained by said retention mechanism, blocks fluid flow through the retention mechanism so that the retention mechanism preferentially retains only one cell. 5. The microfluidic system of claim 4 wherein said release mechanism is for rendering ineffective the retention mechanism by lysing said cell to release intracellular components. 6. The microfluidic system of claim 1, wherein said microfluidic device comprises a layer including a material selected from the group consisting of elastomers, polydimethylsiloxane, plastic, polystyrene, polypropylene, polycarbonate, glass, ceramic, silicon, sol-gels, metal, metalloids, metal oxides, biological polymers, mixtures thereof, particles, proteins, gelatins, polylysine, serum albumin, collagen, nucleic acids, and microorganisms. 7. The microfluidic system of claim 1 further comprising a treatment mechanism in communication with said retention mechanism for selectively treating said cell to produce a treatment response while being retained within said retention mechanism; and, a measurement mechanism for measuring said treatment response, if any, of said cell while said cell is stationary. 8. The microfluidic system of claim 7, wherein said treatment mechanism exposes said particle to a reagent or physical condition. 9. The microfluidic system of claim 7 wherein the fluid sample contains a eukaryotic cell that is labeled with a dye, the treatment mechanism is a light source for illuminating the labeled cell to produce a detectable signal while said labeled cell is stationary and being retained by said retention mechanism; and the measurement mechanism is for measuring said detectable signal, if any, emitted by said labeled cell in response to the illumination while said labeled cell is stationary. 10. The microfluidic system of claim 9, wherein said dye is a fluorophore and the measurement mechanism is a detector associated with said microfluidic device that detects fluorescence. 11. A microfluidic system for cell manipulation comprising: (a) an input mechanism for introducing a fluid sample containing a plurality of eukaryotic cells;(b) a microfluidic passage in fluid communication with said input mechanism;(c) a flow-based positioning mechanism in fluid communication with said microfluidic passage, said positioning mechanism for positioning at least one cell of the plurality of cells in a retention mechanism while contained in said fluid sample; wherein the retention mechanism is for retaining one individual cell upon being positioned by said positioning mechanism,wherein said retention mechanism is configured to retain a single cell and comprises a physical barrier that restricts longitudinal cell movement along the direction of fluid flow and restricts orthogonal cell movement, andwherein the flow-based positioning mechanism comprises a dividing flow site formed by a junction of the microfluidic passage, a first outlet channel, and a second outlet channel, such that when the fluid sample containing the plurality of eukaryotic cells is introduced, said one individual cell is delivered to and retained by the retention mechanism, at least some unretained cells flow through the first outlet channel, and at least some unretained cells flow through the second outlet channel; and,(d) a release mechanism for releasing said cell from said retention mechanism,wherein said release mechanism is for rendering ineffective the retention mechanism by lysing said single cell to release intracellular components. 12. The microfluidic system of claim 11 wherein said single cell, when retained by said retention mechanism, blocks fluid flow through the retention mechanism so that the retention mechanism preferentially retains only one cell. 13. The microfluidic system of claim 11, wherein said microfluidic device comprises a layer including a material selected from the group consisting of elastomers, polydimethylsiloxane, plastic, polystyrene, polypropylene, polycarbonate, glass, ceramic, silicon, sol-gels, metal, metalloids, metal oxides, biological polymers, mixtures thereof, particles, proteins, gelatins, polylysine, serum albumin, collagen, nucleic acids, and microorganisms. 14. The microfluidic system of claim 11, wherein said microfluidic passage is less than about 500 micrometers wide. 15. The microfluidic system of claim 11 further comprising a treatment mechanism in communication with said retention mechanism for selectively treating said single cell to produce a treatment response while being retained within said retention mechanism; and, a measurement mechanism for measuring said treatment response, if any, of said cell while said cell is stationary. 16. The microfluidic system of claim 15 wherein the fluid sample contains a eukaryotic cell that is labeled with a dye, the treatment mechanism is a light source for illuminating the labeled cell to produce a detectable signal while said labeled cell is stationary and being retained by said retention mechanism; and the measurement mechanism is for measuring said detectable signal, if any, emitted by said labeled cell in response to the illumination while said labeled cell is stationary. 17. The microfluidic system of claim 16, wherein said dye is a fluorophore and the measurement mechanism is a detector associated with said microfluidic device that detects fluorescence. 18. The microfluidic system of claim 15, wherein said treatment mechanism exposes said single cell to a reagent or physical condition.
Copyright KISTI. All Rights Reserved.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.