Method of isolation of polyhydroxyalkanoates (PHAs) from biomass fermented by microorganisms producing polyhydroxyalkanoates (PHAs) and/or from biomass containing at least one crop-plant producing polyhydroxyalkanoates
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C08G-063/02
C08G-063/90
출원번호
US-0315895
(2015-06-02)
등록번호
US-9944748
(2018-04-17)
우선권정보
CZ-2014-384 (2014-06-03)
국제출원번호
PCT/CZ2015/000056
(2015-06-02)
국제공개번호
WO2015/185024
(2015-12-10)
발명자
/ 주소
Trejbal, Jiri
Zapletal, Martin
출원인 / 주소
VYSOKA SKOLA CHEMICKO-TECH-NOLOGICKA V PRAZE
대리인 / 주소
Dority & Manning, P.A.
인용정보
피인용 횟수 :
0인용 특허 :
7
초록▼
A method of isolation of polyhydroxyalkanoates from biomass pre-cleans the biomass in a first extraction process by extracting components of the biomass other than polyhydroxyalkanoates. In a second extraction process, polyhydroxyalkanoates are extracted from a solid phase of the biomass obtained in
A method of isolation of polyhydroxyalkanoates from biomass pre-cleans the biomass in a first extraction process by extracting components of the biomass other than polyhydroxyalkanoates. In a second extraction process, polyhydroxyalkanoates are extracted from a solid phase of the biomass obtained in the first extraction process by adding an extraction agent to pre-cleaned biomass based on chlorinated hydrocarbon, carrying out the extraction for 5 to 90 minutes at a temperature in the range of 20 to 120° C., separating the extract containing the polyhydroxyalkanoates from the extraction solution, and feeding the extract to a circulation loop filled with water having a temperature from 20 to 120° C. or a mixture of water and from up to 20% by weight of the extraction agent based on chlorinated hydrocarbon used for the extraction of polyhydroxyalkanoates, wherein the extraction agent is removed from this extract and polyhydroxyalkanoates precipitate.
대표청구항▼
1. A method of isolation of polyhydroxyalkanoates from biomass, wherein the biomass is either fermented by microorganisms that produce polyhydroxyalkanoates and is first inspissated by isolation from a fermentation medium to a dry matter content of at least 20% or the biomass contains at least one c
1. A method of isolation of polyhydroxyalkanoates from biomass, wherein the biomass is either fermented by microorganisms that produce polyhydroxyalkanoates and is first inspissated by isolation from a fermentation medium to a dry matter content of at least 20% or the biomass contains at least one crop-plant that produces polyhydroxyalkanoates, the method comprising: pre-cleaning the biomass in a first extraction process by extracting components of the biomass other than polyhydroxyalkanoates by: (a) adding to the biomass an extraction agent based on alkyl alcohol having 2 to 4 carbon atoms in the chain in a weight ratio of biomass:extraction agent from 1:0.5 to 1:5;(b) carrying out the extraction for 5 to 90 minutes at a temperature in the range of 20 to 120° C.;(c) separating the components other than polyhydroxyalkanoates from the extraction solution by any one or combination of filtration, decantation, or centrifugation;(d) removing a remainder of the extraction agent from the biomass by distillation from an aqueous solution, stripping with water vapour, or drying;in a second extraction process, extracting polyhydroxyalkanoates from a solid phase of the biomass obtained in the first extraction process by: (e) adding an extraction agent based on chlorinated hydrocarbon to the biomass in a weight ratio biomass:extraction agent from 1:5 to 1:20;(f) carrying out the extraction for 5 to 90 minutes at a temperature in the range of 20 to 120° C.;(g) separating the extract containing the polyhydroxyalkanoates from the extraction solution by any one or combination of filtration, decantation, or centrifugation;(h) feeding the extract to a circulation loop filled with water having a temperature from 20 to 120° C. or a mixture of water and from up to 20% by weight of the extraction agent based on chlorinated hydrocarbon used for the extraction of polyhydroxyalkanoates, wherein the extraction agent is removed from this extract and polyhydroxyalkanoates precipitate. 2. The method of isolation of polyhydroxyalkanoates from biomass according to the claim 1, wherein the first extraction process runs in at least two stages, wherein each stage is carried out for 5 to 90 minutes at a temperature of 20 to 120° C., and before each succeeding stage the solid phase from the preceding stage is concentrated by any one or combination of decantation, filtration, or centrifugation. 3. The method of isolation of polyhydroxyalkanoates from biomass according to the claim 2, wherein the stages of the first extraction process are carried out in a mutually countercurrent operation. 4. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the extraction agent based on alkyl alcohol having 2 to 4 carbon atoms in the chain in the first extraction process is added to the biomass in a weight ratio from 1:2 to 1:3. 5. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the first extraction process using an extraction agent based on alkyl alcohol having 2 to 4 carbon atoms in the chain is carried out for 20 to 40 minutes. 6. The method of isolation of polyhydroxyalkanoates from biomass according claim 1, wherein the extraction agent based on alkyl alcohol having 2 to 4 carbon atoms in the chain in the first extraction process is an extraction agent from the group of any one or combination of ethanol, propanol, isopropyl alcohol, butanol, isobutyl alcohol, and tert-butyl alcohol. 7. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the second extraction process runs in at least two stages, wherein each stage is carried out for 5 to 90 minutes at a temperature of 20 to 120° C., and before each succeeding stage the solid phase from the preceding stage is concentrated by any one or combination of ecantation, filtration, or centrifugation. 8. The method of isolation of polyhydroxyalkanoates from biomass according to the claim 7, wherein the stages of the second extraction process are carried out in a mutually countercurrent operation. 9. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the second extraction process using an extraction agent based on chlorinated hydrocarbon is carried out for 20 to 40 minutes. 10. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the extraction agent based on chlorinated hydrocarbon in the second extraction process is an extraction agent from the group of any one or combination of dichlormethan, chloroform, tetrachlormethan, or dichlorethan. 11. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the solid phase of the biomass in the second extraction process is diluted with water before distillation of the extraction agent in a ratio of 1:2 to 1:10, and distillation from this aqueous solution is performed in a rectification column at a pressure of 0.1 to 6 bar. 12. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein stripping with water vapour to remove the extraction agent in step (d) is carried out in a rectification column at a pressure 0.1 to 6 bar. 13. The method of isolation of polyhydroxyalkanoates from biomass according to claim 1, wherein the extract containing polyhydroxyalkanoates is concentrated by evaporating off the extraction agent to reach a concentration of polyhydroxyalkanoates 5 to 10% prior to being fed into the circulation loop. 14. The method of isolation of polyhydroxyalkanoates from biomass according to the claim 13, wherein for evaporating off the extraction agent based on chlorinated hydrocarbon in the circulation loop, condensation heat of vapour of the extraction agent obtained during the concentration of the extract containing polyhydroxyalkanoates is utilized.
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이 특허에 인용된 특허 (7)
Lafferty ; Robert M. ; Heinzle ; Elmar, Cyclic carbonic acid esters as solvents for poly-(.beta.-hydroxybutyric aci d.
Vanlautem Nol (Wavre BEX) Gilain Jacques (Brussels BEX), Process for extracting poly-beta-hydroxybutyrates by means of a solvent from an aqueous suspension of microorganisms.
Blauhut Wilfried (Linz ATX) Gierlinger Wolfgang (Linz ATX) Strempfl Friedrich (Linz ATX), Process for obtaining a polyhydroxyalkanoate from the cell material of a microogranism.
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