[특허]Pluripotent stem cell culture on micro-carriers

Pluripotent stem cell culture on micro-carriers 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-005/00 C12N-005/071 C12N-005/0735
출원번호	US-0631019 (2015-02-25)
등록번호	US-9969972 (2018-05-15)
발명자 / 주소	Nelson, Shelley
출원인 / 주소	Janssen Biotech, Inc.
대리인 / 주소	Gianneschi, Lois A.
인용정보	피인용 횟수 : 0 인용 특허 : 76

초록

The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.

대표청구항 ▼

1. A method to differentiate human pluripotent stem cells to insulin-expressing cells on micro-carriers comprising the steps of: a. attaching the human pluripotent stem cells to a volume of dextran micro-carriers;b. differentiating the human pluripotent stem cells on the dextran microcarriers to definitive endoderm cells using Activin A;c. differentiating the definitive endoderm cells on the dextran microcarriers into pancreatic endoderm cells;d. differentiating the pancreatic endoderm cells on the dextran microcarriers into pancreatic endocrine cells; ande. differentiating the pancreatic endocrine cells on the dextran microcarriers into insulin-expressing cells, wherein the step of attaching comprises culturing the human pluripotent stem cells in medium containing a Rho kinase inhibitor at a concentration of between about 0.25 μM to about 10 μM. 2. The method of claim 1, wherein the micro-carriers are coated with denatured collagen. 3. The method of claim 1, wherein the dextran micro-carriers are agitated. 4. The method of claim 1, wherein the Rho kinase inhibitor is Y27632 or Glycyl-H-1152 dihydrochloride. 5. The method of claim 1, wherein the step of attaching comprises culturing the human pluripotent stem cells in medium containing from about 1 μM to about 10 μM of the Rho kinase inhibitor Y27632. 6. The method of claim 1, wherein the attaching comprises culturing the human pluripotent stem cells in medium containing from about 0.25 μM to about 5 μM of the Rho kinase Glycyl-H-1152 dihydrochloride. 7. The method of claim 1, wherein the step of attaching comprises seeding at least 3.0×104 cells/cm2. 8. The method of claim 1, wherein the method further comprises releasing the pancreatic endocrine cells by treatment with an enzyme. 9. A method to differentiate human pluripotent stem cells to pancreatic endoderm cells on micro-carriers comprising the steps of: a. differentiating human pluripotent stem cells attached to dextran microcarriers to definitive endoderm cells using Activin A; andb. differentiating the definitive endoderm cells on the dextran microcarriers into pancreatic endoderm cells, wherein the human pluripotent stem cells were attached to the dextran microcarriers using a medium containing a Rho kinase inhibitor at a concentration of between about 0.25 μM to about 10 μM. 10. The method of claim 9, wherein the method further comprises differentiating the pancreatic endoderm cells on the dextran microcarriers into pancreatic endocrine cells. 11. The method of claim 10, further comprising differentiating the pancreatic endocrine cells on the dextran microcarriers into insulin-expressing cells. 12. The method of claim 9, wherein the dextran micro-carriers are coated with denatured collagen. 13. The method of claim 9, wherein the micro-carriers are agitated. 14. The method of claim 9, wherein the Rho kinase inhibitor is Y27632 or Glycyl-H-1152 dihydrochloride. 15. A method to differentiate human pluripotent stem cells to pancreatic endoderm cells on micro-carriers comprising the steps of: a. differentiating human pluripotent stem cells attached to dextran microcarriers to definitive endoderm cells using Activin A; andb. differentiating the definitive endoderm cells on the dextran microcarriers into pancreatic endoderm cells.

이 특허에 인용된 특허 (76)

Shenvi, Ashokkumar B., .alpha.-Aminoboronic acid derivatives.
상세보기
Scott, Malcolm K.; Sanfilippo, Pauline J.; Fitzpatrick, III, Louis J.; Cordova, Richard; Pan, Kevin; Meschino, Joseph, Anti-inflammatory compounds.
상세보기
Bathurst, Ian C.; Foehr, Matthew W., Aqueous anti-apoptotic compositions.
상세보기
Yi, Chin-Feng; Gosiewska, Anna; Roweton, Susan, Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering.
상세보기
Adams, Julian; Ma, Yu-Ting; Stein, Ross; Baevsky, Matthew; Grenier, Louis; Plamondon, Louis, Boronic ester and acid compositions.
상세보기
Adams Julian ; Ma Yu-Ting ; Stein Ross ; Baevsky Matthew ; Grenier Louis ; Plamondon Louis, Boronic ester and acid compounds.
상세보기
Adams Julian ; Ma Yu-Ting ; Stein Ross ; Baevsky Matthew ; Grenier Louis ; Plamondon Louis, Boronic ester and acid compounds, synthesis and uses.
상세보기
Adams Julian ; Ma Yu-Ting ; Stein Ross ; Baevsky Matthew ; Grenier Louis ; Plamondon Louis, Boronic ester and acid compounds, synthesis and uses.
상세보기
Tsang,Wen Ghih; Zheng,Tianli; Liu,Wei, CD56 positive human adult pancreatic endocrine progenitor cells.
상세보기
Coon Hayden G. ; Ambesi-Impiombato Francesco Saverio,ITX ; Curcio Francesco,ITX, Cell cultures of and cell culturing method for nontransformed pancreatic, thyroid, and parathyroid cells.
상세보기
Chatelier Ronald C. (9 Apple Gve Bayswater ; Victoria 3168 AUX) Griesser Hans J. (20 View Road The Patch ; Victoria 3792 AUX) Steele John G. (7 The Carriageway North Rocks ; NSW 2151 AUX) Johnson Gra, Cell growth substrates.
상세보기
Marshak Daniel R. ; Holecek James J., Chemically defined medium for human mesenchymal stem cells.
상세보기
Brown, Kelly R.; Zimmerman, Mark C.; Li, Yufu, Composite scaffold with post anchor for the repair and regeneration of tissue.
상세보기
Xu, Chunhui; Gold, Joseph D., Conditioned media for propagating human pluripotent stem cells.
상세보기
Steele John Gerard,AUX ; Holden Brien A.,AUX ; Sweeney Deborah,AUX ; O'Leary Dan,AUX ; Schindhelm Klaus,AUX ; Vannas Antti,FIX ; Johnson Graham,AUX, Corneal onlays.
상세보기
Mandalam,Ramkumar; Xu,Chunhui; Gold,Joseph D.; Carpenter,Melissa K., Culture system for rapid expansion of human embryonic stem cells.
상세보기
Thomson,James A.; Ludwig,Tenneille, Culturing human embryonic stem cells in medium containing pipecholic acid and gamma amino butyric acid.
상세보기
D'Amour, Kevin Allen; Agulnick, Alan D.; Baetge, Emmanuel E., Definitive endoderm.
상세보기
D'Amour,Kevin Allen; Agulnick,Alan D.; Baetge,Emmanuel E., Definitive endoderm.
상세보기
Odorico, Jon; Kahan, Brenda; Treff, Nathan, Differentiation of stem cells to endoderm and pancreatic lineage.
상세보기
Fisk,Gregory J.; Inokuma,Margaret S., Endoderm cells from human embryonic stem cells.
상세보기
Vyakarnam Murty N. ; Zimmerman Mark C. ; Scopelianos Angelo George ; Chun Iksoo ; Melican Mora C. ; Bazilio Clairene A. ; Roller Mark B. ; Gorky David V., Foam composite for the repair or regeneration of tissue.
상세보기
Gupta, Shanker Lal, Formulation of boronic acid compounds.
상세보기
Gupta, Shanker Lal, Formulation of boronic acid compounds.
상세보기
Vacanti Joseph P. (Winchester MA) Freeman Michael R. (Boston MA), Genitourinary cell-matrix structure for implantation into a human and a method of making.
상세보기
Lakshmi Rambhatla ; Melissa K. Carpenter, Hepatocyte lineage cells derived from pluripotent stem cells.
상세보기
Evans Ronald M. (San Diego CA) Rosenfeld Michael G. (San Diego CA) Cerelli Gail (Cardiff CA) Mayo Kelly E. (Wilmette IL) Spiess Joachim (Encinitas CA) Rivier Jean E. F. (La Jolla CA) Vale ; Jr. Wylie, Human inhibin.
상세보기
Fernandez Mireya,CLX ; Minguell Jose J.,CLX, Human mesenchymal stem cells from peripheral blood.
상세보기
Penelope E. Roberts ; Jennie Powell Mather, Human pancreatic epithelial progenitor cells and methods of isolation and use thereof.
상세보기
James R. Musick ; John Charles Gill, IV ; Bruce P. Burnett ; Tammy E. Hedlund, Immortalized cell lines and methods of making the same.
상세보기
Agulnick, Alan D., In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells.
상세보기
Peck Ammon B. ; Cornelius Janet G., In vitro growth of functional islets of Langerhans.
상세보기
Peck Ammon B. ; Cornelius Janet G., In vitro growth of functional islets of Langerhans and in vivo uses thereof.
상세보기
Thayer Richard L. (Billings MT), Inorganic support for culture media.
상세보기
Herrero-Vanrell Rocio,ESX ; Refojo Miguel F., Intravitreal microsphere drug delivery and method of preparation.
상세보기
Fisk,Gregory J.; Inokuma,Margaret S., Islet cells from human embryonic stem cells.
상세보기
Thomson,James A.; Ludwig,Tenneille, Medium containing pipecholic acid and gamma amino butyric acid and culture of embryonic stem cells.
상세보기
Mandalam,Ramkumar; Xu,Chunhui, Medium for growing human embryonic stem cells.
상세보기
McIntosh Kevin R. ; Mosca Joseph D. ; Klyushnenkova Elena N., Mesenchymal stem cells for prevention and treatment of immune responses in transplantation.
상세보기
Evans, Glen A., Method for assembly of a polynucleotide encoding a target polypeptide.
상세보기
Bryhan, Marie D.; Gagnon, Paul E.; LaChance, Oliva V.; Shen, Zhong-he; Wang, Hongming, Method for creating a cell growth surface on a polymeric substrate.
상세보기
Evans, Glen A., Method for the complete chemical synthesis and assembly of genes and genomes.
상세보기
McIntosh Kevin ; Klyushnenkova Elena, Method of preparing suppressor T cells with allogeneic mesenchymal stem cells.
상세보기
Bodnar, Andrea G.; Chiu, Choy-Pik; Gold, Joseph D.; Inokuma, Margaret; Murai, James T.; West, Michael D., Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture.
상세보기
Dalton, Stephen; Sheppard, Allan; Jones, Karen; Baetge, E. Edward; D'Amour, Kevin A.; Agulnick, Alan D., Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors.
상세보기
Bonner-Weir, Susan; Taneja, Monica, Methods of making pancreatic islet cells.
상세보기
Martinson, Laura; Kroon, Evert; D'Amour, Kevin; Baetge, Emmanuel Edward, Methods of producing pancreatic hormones.
상세보기
Martinson, Laura; Kroon, Evert; D'Amour, Kevin; Baetge, Emmanuel Edward, Methods of producing pancreatic hormones.
상세보기
Caplan Arnold I. ; Haynesworth Stephen E., Monoclonal antibodies for human mesenchymal stem cells.
상세보기
Schilder Lothar (Hamburg DEX) Dahlke Hermann (Hamburg DEX), Multi-layered implant.
상세보기
Haas, Martin, Multipotent amniotic fetal stem cells.
상세보기
Mason Anthony J. (San Francisco CA) Seeburg Peter H. (Heidelberg DEX), Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic aci.
상세보기
Mason Anthony J. ; Seeburg Peter H., Nucleic acid encoding the mature .beta..sub.B chain of inhibin and method for synthesizing polypeptides using such nucl.
상세보기
Mason Anthony J. (San Francisco CA) Seeburg Peter H. (San Francisco CA), Nucleic acid encoding the mature a 상세보기

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC

저장형식

Text(ASCII format)
Excel format
PIAS분석(.xls)

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

연합인증

Pluripotent stem cell culture on micro-carriers 원문보기

초록

대표청구항 ▼

연구과제 타임라인

이 특허에 인용된 특허 (76)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Pluripotent stem cell culture on micro-carriers 원문보기

초록

대표청구항 ▼

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

이 특허에 인용된 특허 (76)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트