The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particul
The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.
대표청구항▼
1. An isolated thermolysin variant, wherein said thermolysin variant has at least 95% amino acid identity with the amino acid sequence set forth in SEQ ID NO: 3 and comprises a substitution at position 65 of the amino acid sequence set forth as SEQ ID NO: 3, wherein said substitution is selected fro
1. An isolated thermolysin variant, wherein said thermolysin variant has at least 95% amino acid identity with the amino acid sequence set forth in SEQ ID NO: 3 and comprises a substitution at position 65 of the amino acid sequence set forth as SEQ ID NO: 3, wherein said substitution is selected from the group consisting of S065I, S065T, S065C, S065D, S065E, S065V, S065W, and S065Y. 2. The isolated thermolysin variant of claim 1, wherein said variant has an improvement in thermostability as compared to wild-type Geobacillus caldoproteolyticus thermolysin set forth in SEQ ID NO:3, wherein said improvement in stability is determined by the stability assay set forth in Example 1C. 3. A cleaning composition comprising the isolated thermolysin variant of claim 1 and a detergent. 4. The composition of claim 3, wherein said composition further comprises at least one calcium ion and/or zinc ion. 5. The composition of claim 3, wherein said composition further comprises at least one stabilizer. 6. The composition of claim 5, wherein said stabilizer is chosen from borax, glycerol, zinc ions, calcium ions, and calcium formate. 7. The composition of claim 5, wherein said stabilizer is at least one competitive inhibitor that stabilizes the at least one thermolysin in the presence of an anionic surfactant. 8. The composition of claim 3, further comprising at least one additional enzyme or enzyme derivative chosen from proteases, amylases, lipases, mannanases, pectinases, cutinases, oxidoreductases, hemicellulases, and cellulases. 9. The composition of claim 3, wherein said composition comprises at least about 0.0001 weight percent of said thermolysin variant. 10. The composition of claim 3, wherein said composition comprises from about 0.001 to about 0.5 weight percent of said thermolysin variant. 11. The composition of claim 3, wherein said composition is a liquid. 12. A method of cleaning, comprising the step of contacting a surface and/or an article comprising a fabric with a cleaning composition comprising the isolated thermolysin variant of claim 3.
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이 특허에 인용된 특허 (38)
Bragg Charles D. (Newcastle upon Tyne GB2), Bleach catalyst compositions and use thereof in laundry bleaching and detergent compositions.
Perkins Christopher Mark ; Labeque Regine,BEX ; Williams Barbara Kay ; Johnston James Pyott,BEX ; Kitko David Johnathan ; Burckett-St. Laurent James Charles Theophile Roger ; Burns Michael Eugene, Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids.
Miracle Gregory S. (Hamilton OH) Burns Michael E. (Hamilton OH) Kellett Patti J. (Cincinnati OH) Burckett-St. Laurent James C. T. R. (Cincinnati OH), Color-safe bleach boosters, compositions and laundry methods employing same.
Miracle Gregory S. (Hamilton OH) Sivik Mark R. (Fairfield OH) Kellett Patti J. (Cincinnati OH), Detergent compositions comprising multiperacid-forming bleach activators.
Blue Emily Keller ; Chiu Chung-Wai ; Hussain Zahera ; Shah Himanshu ; Trubiano Paul, Glucoamylase converted starch derivatives and their use as emulsifying and encapsulating agents.
Dinniwell Alan R. (Mason OH) Wasserman Matthew I. (Cincinnati OH), Low dosage detergent composition containing optimum proportions of agglomerates and spray dried granules for improved fl.
Bott Richard Ray (Burlingame CA) Caldwell Robert Mark (San Francisco CA) Cunningham Brian C. (Piedmont CA) Estell David Aaron (Mountain View CA) Power Scott Douglas (San Bruno CA) Wells James Allen (, Modified subtilisins having amino acid alterations.
Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Gelfand David H. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme.
Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
Nassano David Robert (Cold Springs KY) Capeci Scott William (North Bend OH), Process for making a high density detergent composition by controlling agglomeration within a dispersion index.
Capeci Scott W. (North Bend OH) Lange John F. (Villa Hills KY) Smith David J. (Kenton GB3) Roberts Nigel S. (Jesmond GB3), Process for making a high density detergent composition in a single mixer/densifier with selected recycle streams for im.
Capeci Scott W. (North Bend OH) Lange John F. (Villa Hills KY) Smith David J. (Kenton GB3) Roberts Nigel S. (Jesmond GB3), Process for making a high density detergent composition which includes selected recycle streams for improved agglomerate.
Capeci Scott W. (North Bend OH) Osborn James W. (Cincinnati OH) Angell Adrian J. W. (Strombeek-Bever BEX) van Dijk Paul (Putte BEX), Process for making high density detergent agglomerates using an anhydrous powder additive.
Del Greco Angela G. (Cincinnati OH) Ruh Anne M. (Cincinnati OH), Process for preparing a free-flowing particulate detergent composition having improved solubility.
Welch Robert G. (Cincinnati OH) Githuku David N. (Mason OH) Hollihan Lester J. (Alexandria KY) Jackson Charles A. (Fayetteville OH), Process for producing detergent agglomerates from high active surfactant pastes having non-linear viscoelastic propertie.
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