Temporal regulation of gene expression by microRNAs
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-015/00
C12N-015/82
출원번호
US-0065416
(2016-03-09)
등록번호
US-9976152
(2018-05-22)
발명자
/ 주소
Allen, Edwards M.
Heisel, Sara E.
Ivashuta, Sergey
Krieger, Elysia K.
Lutke, Jennifer
Meister, Robert J.
Zhang, Yuanji
출원인 / 주소
MONSANTO TECHNOLOGY LLC
대리인 / 주소
Arnold & Porter Kaye Scholer LLP
인용정보
피인용 횟수 :
0인용 특허 :
54
초록▼
This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant
This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
대표청구항▼
1. A recombinant DNA construct transcribable in a plant cell for temporally specific expression of at least one gene, wherein said expression of at least one gene is modulated by a circadian plant miRNA gene that encodes a mature miRNA selected from the group consisting of Gm-miRMON1 (SEQ ID NO. 1),
1. A recombinant DNA construct transcribable in a plant cell for temporally specific expression of at least one gene, wherein said expression of at least one gene is modulated by a circadian plant miRNA gene that encodes a mature miRNA selected from the group consisting of Gm-miRMON1 (SEQ ID NO. 1), Gm-miR393 (SEQ ID NO. 2), Gm-miR159 (SEQ ID NO. 3), Gm-miR390 (SEQ ID NO. 4), Gm-miR156 (SEQ ID NO. 5), and Gm-TAS3 5′D7(+) (SEQ ID NO. 6),wherein said recombinant DNA construct comprises a DNA encoding said at least one gene and a DNA encoding an exogenous miRNA recognition site recognizable by said mature miRNA, andwherein said expression of said at least one gene is decreased during time periods when said mature miRNA is transcribed relative to expression during time periods when said mature miRNA is not transcribed. 2. The recombinant DNA construct of claim 1, wherein: (a) said at least one gene is at least one selected from the group consisting of a gene endogenous to said plant cell, a transgene in said plant cell, and a gene endogenous to a pest or pathogen of a plant; or(b) said at least one gene is at least one selected from the group consisting of coding DNA and non-coding DNA. 3. The recombinant DNA construct of claim 1 further comprising a promoter, wherein said promoter comprises: (a) SEQ ID NO. 30, or(b) at least 50 contiguous nucleotides of SEQ ID NO. 30, or(c) SEQ ID NO. 31, or(d) at least 50 contiguous nucleotides of SEQ ID NO. 31, or(e) SEQ ID NO. 35, or(f) at least 50 contiguous nucleotides of SEQ ID NO. 35, or(g) SEQ ID NO. 36, or(h) at least 50 contiguous nucleotides of SEQ ID NO. 36,and exhibits circadian promoter activity in a plant. 4. A non-natural transgenic plant cell having in its genome the recombinant DNA construct of claim 1. 5. A method of temporally regulating expression of a gene, comprising expressing in a plant the recombinant DNA construct of claim 1, whereby said expression of at least one gene is down-regulated by said circadian miRNA gene of said plant, resulting in temporally specific expression of said at least one gene. 6. The method of claim 5, wherein the DNA construct comprises a promoter operably linked to the DNA encoding said at least one gene, wherein said promoter comprises: (a) SEQ ID NO. 30, or(b) at least 50 contiguous nucleotides of SEQ ID NO. 30, or(c) SEQ ID NO. 31, or(d) at least 50 contiguous nucleotides of SEQ ID NO. 31, or(e) SEQ ID NO. 35, or(f) at least 50 contiguous nucleotides of SEQ ID NO. 35, or(g) SEQ ID NO. 36, or(h) at least 50 contiguous nucleotides of SEQ ID NO. 36,and exhibits circadian promoter activity in a plant, andwherein said expression of at least one gene is substantially nocturnal. 7. A transgenic plant cell containing the recombinant DNA construct of claim 3. 8. A plant containing the transgenic plant cell of claim 7. 9. A plant containing the transgenic plant cell of claim 4.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (54)
Anderson Paul C. ; Chomet Paul S. ; Griffor Matthew C. ; Kriz Alan L., Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction.
John Russell Booth, Jr. ; Richard Martin Broglie ; William Dean Hitz ; Anthony John Kinney ; Susan Knowlton ; Scott Anthony Sebastian, Gene combinations that alter the quality and functionality of soybean oil.
Jonathan Edward Lightner ; John Joseph Okuley ; William Hitz ; Anthony John Kinney ; Luis Perez-Grau ; Narendra S. Yadav, Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants.
Lightner, Jonathan Edward; Okuley, John Joseph; Hitz, William Dean; Kinney, Anthony John; Perez-Grau, Luis; Yadav, Narendra S., Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants.
Barry Gerard F. (St. Louis MO) Kishore Ganesh M. (Chesterfield MO) Padgette Stephen R. (Grover MO) Stallings William C. (Glencoe MO), Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases.
Matsunaga Etsuko,JPX ; Kasahara Takehide,JPX ; Sugita Koichi,JPX ; Ebinuma Hiroyasu,JPX, Method for introducing a gene into a plant using an adventitious bud redifferentiation gene under the control of a light-inducible promoter as a selectable marker gene, and vector for introducing a g.
Corbin David R. (Chesterfield MO) Greenplate John T. (Manchester MO) Jennings Michael G. (Chesterfield MO) Purcell John P. (Ballwin MO) Sammons Robert D. (Defiance MO), Method of controlling insects.
Michael G. Koziel ; Nalini M. Desai ; Kelly S. Lewis ; Vance C. Kramer ; Gregory W. Warren ; Stephen V. Evola ; Lyle D. Crossland ; Martha S. Wright ; Ellis J. Merlin ; Karen L. Launis ; St, Method of producing transgenic maize using direct transformation of commercially important genotypes.
Adams Thomas R. (No. Stonington CT) Chambers Sheryl A. (Groton CT) Daines Richard J. (Ledyard CT) Gordon-Kamm William J. (Stonington CT) Kausch Albert P. (Stonington CT) Lemaux Peggy G. (Mystic CT) M, Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof.
Paul C. Anderson ; Christopher E. Flick ; William J. Gordon-Kamm ; Albert P. Kausch ; Catherine J. Mackey ; Emil M. Orozco ; Peter Orr ; Michael A. Stephens ; David A. Walters ; Donald S. Wa, Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof.
Christou Paul (Madison WI) McCabe Dennis (Middleton WI) Swain William F. (Madison WI) Barton Kenneth A. (Middleton WI), Particle-mediated transformation of soybean plants and lines.
Sanders Jan W.,NLX ; Kok Jan,NLX ; Venema Gerard,NLX ; Ledeboer Adrianus M,NLX, Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein.
Brian J. Martinell ; Lori S. Julson ; Carol A. Emler ; Yong Huang ; Dennis E. McCabe ; Edward J. Williams, Soybean agrobacterium transformation method.
Allen, Edwards; Heisel, Sara Elizabeth; Ivashuta, Sergey; Krieger, Elysia Katherine; Lutke, Jennifer Lynn; Meister, Robert Joseph; Zhang, Yuanji, Temporal regulation of gene expression by MicroRNAs.
Rangan, Thirumale S.; Anderson, David M.; Rajasekaran, Kanniah; Grula, John W.; Hudspeth, Richard Lorne; Yenofsky, Richard L., Transformation of cotton plants.
Cheo,David; Brasch,Michael A.; Temple,Gary F.; Hartley,James L.; Byrd,Devon R. N., Use of multiple recombination sites with unique specificity in recombinational cloning.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.