Asynchronous magnetic bead rotation (AMBR) microviscometer for analysis of analytes
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
G01N-011/16
G01N-011/10
G01N-011/00
출원번호
US-0532907
(2014-11-04)
등록번호
US-9983110
(2018-05-29)
발명자
/ 주소
Kopelman, Raoul
Burns, Mark A.
Li, Yunzi
출원인 / 주소
THE REGENTS OF THE UNIVERSITY OF MICHIGAN
대리인 / 주소
Marshall, Gerstein & Borun LLP
인용정보
피인용 횟수 :
0인용 특허 :
50
초록▼
The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes su
The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes surrounding the paramagnetic bead. Optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of analyte concentration. The results demonstrate the feasibility of viscosity-based analyte detection using AMBR in microscale aqueous volumes.
대표청구항▼
1. A method of detecting a chemical change in state of an analyte in a sample comprising: (a) contacting the sample with a magnetic particle;(b) measuring a first instantaneous non-linear rotation rate of the magnetic particle in the sample at a first time; and(c) measuring a second instantaneous no
1. A method of detecting a chemical change in state of an analyte in a sample comprising: (a) contacting the sample with a magnetic particle;(b) measuring a first instantaneous non-linear rotation rate of the magnetic particle in the sample at a first time; and(c) measuring a second instantaneous non-linear rotation rate of the magnetic particle in the sample at a second time;wherein a difference between the first instantaneous rotation rate relative to the second instantaneous rotation rate indicates a change in viscosity in the sample, which indicates the change in state of the analyte in the sample,wherein the chemical change in state is selected from the group consisting of nucleic acid synthesis, protein synthesis, nucleic acid hydrolysis, nucleic acid ligation, and protein hydrolysis. 2. The method of claim 1 wherein the analyte is a monomer of a biopolymer. 3. The method of claim 1 wherein the analyte is selected from the group consisting of a cell, a nucleic acid, a protein, a carbohydrate, a lipid, and an amino acid. 4. The method of claim 1 wherein the nucleic acid synthesis is by polymerase chain reaction (PCR). 5. The method of claim 1 wherein the protein synthesis is by in vitro translation. 6. The method of claim 1 wherein the protein synthesis occurs in a cell. 7. The method of claim 1 wherein the nucleic acid hydrolysis is catalyzed by a type I or a type II restriction endonuclease. 8. The method of claim 1 wherein the chemical change is formation or loss of a nucleic acid hybrid, a blood clot, or a ligand-receptor interaction, a nucleic acid-protein interaction, a protein-lipid interaction, a protein-carbohydrate interaction, an antibody-antigen interaction. 9. The method of claim 2 wherein the change in viscosity indicates a change in concentration of analyte in the sample. 10. The method of claim 2 wherein the change in viscosity indicates a change in average length of analyte in the sample. 11. The method of claim 1 wherein the change in viscosity is measured in real time. 12. The method of claim 1 wherein the change in viscosity is from about 0.01 centipoise (cP) to about 10 cP. 13. The method of claim 12 wherein the change in viscosity is from about 0.1 cP to about 2 cP. 14. A method of detecting a chemical change in state of an analyte in a sample comprising: (a) contacting the sample with a magnetic particle;(b) measuring a first instantaneous non-linear rotation rate of the magnetic particle in the sample at a first time; and(c) measuring a second instantaneous non-linear rotation rate of the magnetic particle in the sample at a second time;wherein a difference between the first instantaneous rotation rate relative to the second instantaneous rotation rate indicates a change in viscosity in the sample, which indicates the change in state of the analyte in the sample,wherein the chemical change in state is catalyzed by an enzyme. 15. The method of claim 14 wherein the analyte is a monomer of a biopolymer. 16. The method of claim 14 wherein the analyte is selected from the group consisting of a cell, a nucleic acid, a protein, a carbohydrate, a lipid, and an amino acid. 17. The method of claim 14 wherein the enzyme is selected from the group consisting of a polymerase, a nuclease, a hydrolase, a lyase, an oxidase, a peptidase, and a ligase. 18. The method of claim 14 wherein the chemical change is formation or loss of a nucleic acid hybrid, a blood clot, or a ligand-receptor interaction, a nucleic acid-protein interaction, a protein-lipid interaction, a protein-carbohydrate interaction, an antibody-antigen interaction. 19. The method of claim 15 wherein the change in viscosity indicates a change in concentration of analyte in the sample. 20. The method of claim 15 wherein the change in viscosity indicates a change in average length of analyte in the sample. 21. The method of claim 14 wherein the change in viscosity is measured in real time. 22. The method of claim 14 wherein the change in viscosity is from about 0.01 centipoise (cP) to about 10 cP. 23. The method of claim 22 wherein the change in viscosity is from about 0.1 cP to about 2 cP.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (50)
LaBorde,Ronald T.; Taff,Randall N.; Pratt,David M., Accessible assay and method of use.
Smith Lloyd M. (Madison WI) Hood Leroy E. (Pasadena CA) Hunkapiller Michael W. (San Carlos CA) Hunkapiller Tim J. (Pasadena CA), Automated DNA sequencing technique.
Alexander W. Clark ; Paul Gladnick ; Robert E. Armstrong ; Nicholas Bachur ; Klaus W. Berndt ; Dwight Livingston, Automated microbiological testing apparatus and method therefor.
Clark Alexander W. ; Gladnick Paul ; Armstrong Robert E. ; Bachur Nicholas ; Berndt Klaus W. ; Livingston Dwight, Automated microbiological testing apparatus and methods therefor.
Hyman, Jones M.; Matsumura, Paul M.; Jeffrey, Scott R.; Maresch, Martin J.; Thorpe, Thurman C., Device for isolation and surface culture of microorganisms from bulk fluids.
J. Bruce Pitner ; John Jacob Hemperly ; Richard D. Guarino ; Magdalena Wodnicka ; David T. Stitt ; Gregory J. Burrell ; Timothy G. Foley, Jr. ; Patrick Shawn Beaty, Device for monitoring cells.
Pitner, J. Bruce; Hemperly, John Jacob; Guarino, Richard D.; Wodnicka, Magdalena; Stitt, David T.; Burrell, Gregory J.; Foley, Jr., Timothy G.; Beaty, Patrick Shawn, Device for monitoring cells.
Rohr Thomas E. ; Elstrom Tuan A. ; Howard Lawrence V. ; Shain Eric B., Magnetically assisted binding assays utilizing a magnetically responsive reagent.
Kuehnle Adelheid R. (1617 Keeaumoku St. #1008 Honolulu HI 96822) Kuehnle Manfred R. (Waldesruh Rte. 103A ; P.O. Box 1020 New London NH 03257), Magnetophoretic particle delivery method and apparatus for the treatment of cells.
Mehta, Tammy Burd; Kopf-Sill, Anne R.; Parce, J. Wallace; Chow, Andrea W.; Bousse, Luc J.; Knapp, Michael R.; Nikiforov, Theo T.; Gallagher, Steve, Manipulation of microparticles in microfluidic systems.
Michael Bancroft Simmonds ; Kurt Gordon Jensen ; Jost Hermann Diederichs ; Randall Christopher Black, Method and apparatus for making measurements of accumulations of magnetically susceptible particles combined with analytes.
Simmonds, Michael Bancroft; Jensen, Kurt Gordon; Diederichs, Jost Hermann; Black, Randall Christopher, Method and apparatus for making measurements of accumulations of magnetically susceptible particles combined with analytes.
Simmonds, Michael Bancroft; Jensen, Kurt Gordon; Diederichs, Jost Hermann; Black, Randall Christopher, Method and apparatus for making measurements of patterns of magnetic particles in lateral flow membranes and microfluidic systems.
Sager, Ronald E.; Simmonds, Michael B.; Diederichs, Jost H.; Jensen, Kurt G.; Black, Randall C., Method and apparatus for quantitative determination of accumulations of magnetic particles.
Minchole, Ana; Astalan, Andrea P.; Johansson, Christer; Lagerwall-Larsson, Kerstin; Krozer, Anatol, Method and arrangement for detecting changes of a magnetic response in magnetic particles.
Benner Steven A. (Hadlaubstrasse 151 CH-8006 Zurich CHX), Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases.
Monget Daniel (Saint Sorlin en Bugey FRX) Villeval Francois (Saint Genis les Ollieres FRX), Method of bacteriological analysis, and medium for the detection of bacteria of the Salmonella genus.
Black Larry J. (Indianapolis IN) Cullinan George J. (Trafalgar IN), Methods for inhibiting bone loss by treating with aroylbenzothiophenes and estrogen.
Kopelman Raoul ; Clark Heather ; Monson Eric ; Parus Stephen ; Philbert Martin ; Thorsrud Bjorn, Optical fiberless sensors for analyzing cellular analytes.
Weitschies Werner,DEX ; Kotitz Roman,DEX ; Trahms Lutz,DEX ; Bunte Thomas,DEX, Process and compounds for the magnetorelaxometric detection of analytes and use thereof.
Jeffrey, Scott R.; Matsumura, Paul M.; Maresch, Martin J.; Hyman, Jones M.; Thorpe, Thurman C., Sensor device for detecting microorganisms, and method therefor.
Kardos Keith W. ; Niedbala R. Sam ; Burton Jarrett Lee ; Cooper David E. ; Zarling David A. ; Rossi Michel J.,CHX ; Peppers Norman A. ; Kane James ; Faris Gregory W. ; Dyer Mark J. ; Ng Steve Y. ; Sc, Up-converting reporters for biological and other assays.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.