최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
---|---|
국제특허분류(IPC7판) |
|
출원번호 | US-0744889 (2015-06-19) |
등록번호 | US-9988676 (2018-06-05) |
발명자 / 주소 |
|
출원인 / 주소 |
|
대리인 / 주소 |
|
인용정보 | 피인용 횟수 : 0 인용 특허 : 366 |
A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels i
A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.
1. A nucleic acid analyzer, comprising: a microfluidic cartridge module configured to accept at least one microfluidic cartridge comprising: at least one nucleic acid analysis portion, each nucleic acid analysis portion including:a fluidic network defined within the nucleic acid analysis portion, th
1. A nucleic acid analyzer, comprising: a microfluidic cartridge module configured to accept at least one microfluidic cartridge comprising: at least one nucleic acid analysis portion, each nucleic acid analysis portion including:a fluidic network defined within the nucleic acid analysis portion, the fluidic network being configured for micro-liter volumes or less, the fluidic network including at least one frangible seal formed from a depression defined in a fluidic channel with a fluid flow resistance determined by a depth and a width of the depression;a sample input at a beginning of the fluidic network, the sample input having a fitting that is configured to be mated to a complementary fitting of a sample acceptor to form a fluid-tight seal;a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network;an extraction mixture reservoir in the fluidic network, the extraction mixture reservoir being configured to hold an enzymatic mixture for performing nucleic acid extraction on a sample provided by the sample acceptor;a mixing chamber in the fluidic network, the mixing chamber being configured to mix amplification reagents and a portion of an extracted nucleic acid mixture; andan amplification chamber in the fluidic network, the amplification chamber being configured to hold an amplification mixture during nucleic acid amplification;a pressure module configured to be coupled to the plurality of vent ports to effectuate hydrodynamic movement within the fluidic network of the microfluidic cartridge;an extraction thermal module configured to impart temperatures to any of the microfluidic cartridge and the sample acceptor during nucleic acid extraction;an amplification thermal module configured to impart temperatures to the microfluidic cartridge during nucleic acid amplification;a high voltage module configured to apply high voltages on the microfluidic cartridge;a power module configured to provide operation powers to the nucleic acid analyzer;a detection module configured to detect labeled or dyed nucleic acids; anda controller module configured to control the pressure module, the extraction thermal module, the amplification thermal module, the high voltage module, the power module, and the detection module according to a control procedure. 2. The nucleic acid analyzer of claim 1, wherein the pressure module is configured to break one or more frangible seals in the fluidic network and/or actuate one or more valves of the microfluidic cartridge during nucleic acid analysis. 3. The nucleic acid analyzer of claim 1, wherein the pressure module is configured to provide pneumatic positive and/or negative pressure. 4. The nucleic acid analyzer of claim 1, wherein the pressure module includes a hydrodynamic pressure/vacuum system. 5. The nucleic acid analyzer of claim 1, wherein the pressure module includes a solenoid manifold. 6. The nucleic acid analyzer of claim 1, wherein the extraction thermal module is configured to heat the sample acceptor by contact heating. 7. The nucleic acid analyzer of claim 1, wherein the amplification thermal module includes a non-contact heating unit and a temperature sensing unit. 8. The nucleic acid analyzer of claim 7, wherein the non-contact heating unit includes an infrared light source. 9. The nucleic acid analyzer of claim 7, wherein the temperature sensing unit includes an infrared pyrometer to measure temperature of a portion of the microfluidic cartridge. 10. The nucleic acid analyzer of claim 1, wherein the detection module comprises: a laser source unit configured to emit a laser beam;a detector unit configured to detect fluorescence; andan optical unit configured to direct the laser beam to a separation channel to excite fluorescent molecules to emit fluorescence and to then direct fluorescence to the detector unit for detection. 11. The nucleic acid analyzer of claim 10, wherein the optical unit includes a self-calibrating array of confocal optical components configured to perform calibration in conjunction with an alignment dye. 12. The nucleic acid analyzer of claim 1, further comprising a computing module operationally coupled to the controller module, the computing module having a user interface.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.