Agricultural endophyte-plant compositions, and methods of use
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-063/00
A01N-063/02
A01C-001/06
G01N-033/50
A01C-001/00
출원번호
US-0916514
(2014-09-04)
등록번호
US-10136646
(2018-11-27)
우선권정보
WO-PCT/US2014/044427 (2014-06-26)
국제출원번호
PCT/US2014/054160
(2014-09-04)
국제공개번호
WO2015/035099
(2015-03-12)
발명자
/ 주소
Von Maltzahn, Geoffrey
Flavell, Richard Bailey
Toledo, Gerardo V.
Jack, Allison
Johnston, David Morris
Djonovic, Slavica
Marquez, Luis Miguel
Millet, Yves Alain
Sadowski, Craig
Lyford, Jeffrey
Naydich, Alexander
출원인 / 주소
Indigo Ag, Inc.
대리인 / 주소
Fenwick & West LLP
인용정보
피인용 횟수 :
0인용 특허 :
44
초록▼
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant or a Gycine plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial popul
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant or a Gycine plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal or soy seeds and/or cereal or soy seedlings) with heterologous seed-derived bacterial endophytes.
대표청구항▼
1. A method for preparing an agricultural seed preparation, comprising: a) manually or mechanically inoculating a plurality of Glycine agricultural plant seeds with a formulation comprising a purified bacterial population at a concentration of at least 104 CFU/ml in a liquid formulation or at least
1. A method for preparing an agricultural seed preparation, comprising: a) manually or mechanically inoculating a plurality of Glycine agricultural plant seeds with a formulation comprising a purified bacterial population at a concentration of at least 104 CFU/ml in a liquid formulation or at least 104 CFU/gm in a non-liquid formulation, the bacterial population comprising a seed bacterial endophyte comprising a 16S nucleic acid sequence comprising SEQ ID NO: 1446 and exhibiting the ability to produce or induce in a Glycine agricultural plant grown from the inoculated Glycine agricultural plant seed at least one of production of an auxin or production of acetoin, wherein the inoculated seeds and/or the Glycine agricultural plant grown therefrom comprises an increased production of a polypeptide of Aspartate aminotransferase glyoxysomal isozyme AAT1 precursor, wherein the seed bacterial endophyte is present in the formulation in an amount capable of providing a benefit to the inoculated Glycine plant seeds or to the Glycine agricultural plants grown from the inoculated Glycine plant seeds compared to reference Glycine seeds or reference Glycine agricultural plants, wherein the formulation further comprises a stabilizer, a fungicide, a preservative, or a combination thereof; and(b) packaging the inoculated seeds in a container. 2. The method of claim 1, wherein the bacterial endophyte is amplified within a tissue of the Glycine agricultural plant. 3. The method of claim 1, wherein the inoculated seeds are packaged in the container such that they are substantially stable at about 25 degrees C. for at least 7 days. 4. The method of claim 1, wherein the bacterial endophyte comprises the ability to induce in the Glycine agricultural plant production of an auxin and production of acetoin. 5. The method of claim 1, wherein the inoculating in (a) comprises spraying, immersing, coating, encapsulating, injecting or dusting the seeds with the formulation. 6. The method of claim 1, wherein the purified bacterial population comprises a plurality of seed bacterial endophyte entities. 7. The method of claim 1, wherein the inoculating results in a concentration of at least 104 CFU of the bacterial endophyte on the surface of the seed. 8. The method of claim 1, wherein the bacterial population is obtained from an interior seed compartment and/or wherein the bacterial population comprises a seed bacterial endophyte. 9. The method of claim 1, wherein the bacterial population is obtained from a plant species other than the seeds to which the formulation is contacted. 10. The method of claim 1, wherein the bacterial population is obtained from a plant species other than the seeds to which the formulation is contacted, and wherein the bacterial population is propagated in culture. 11. The method of claim 1, wherein the bacterial population is obtained from a plant cultivar or a seed thereof different from the cultivar of the seeds to which the formulation is contacted. 12. The method of claim 1, wherein the bacterial population is obtained from a surface sterilized seed. 13. The method of claim 1, wherein the benefit is increased tolerance to drought. 14. An agricultural seed preparation prepared by the method of claim 1. 15. A method of making a seed treatment, the method comprising: (a) inoculating an isolated cultured bacterial endophyte cell or spore comprising a 16S nucleic acid sequence comprising SEQ ID NO: 1446 into a liquid medium;(b) culturing the bacterial endophyte cell or spore in the medium at a temperature of about 4 to about 37 degrees C. until the cell or spore density in the medium is at least 104 CFU/mL; and(c) collecting the vegetative cells or spores under conditions suitable to produce a seed treatment.
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von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
von Maltzahn, Geoffrey; Flavell, Richard Bailey; Toledo, Gerardo V.; Djonovic, Slavica; Marquez, Luis Miguel; Johnston, David Morris; Millet, Yves Alain; Lyford, Jeffrey; Naydich, Alexander; Sadowski, Craig, Methods of use of seed-origin endophyte populations.
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